Click here to switch to the map view.

The map label for this gene is HisF

Identifier: 20808523

GI number: 20808523

Start: 2050128

End: 2050889

Strand: Reverse

Name: HisF

Synonym: TTE2133

Alternate gene names: 20808523

Gene position: 2050889-2050128 (Counterclockwise)

Preceding gene: 20808524

Following gene: 20808522

Centisome position: 76.26

GC content: 38.32

Gene sequence:

>762_bases
ATGCTTGCAAAGAGAATAATACCATGTCTGGATGTTAAAGATGGAAGAGTAGTAAAAGGAATAAATTTTGTAAATTTAAA
GGATGCTGGAGACCCTGTTGAAATAGCTGAAAGGTACAATGAATTAGGAGCCGATGAGCTTGTATTTCTGGATATTACAG
CTTCCTATGAAAAAAGAAAAATTATGATAGATGTTGTAAAGAGAACATCAGAAAAGGTGTTTATCCCTTTGACTGTAGGG
GGAGGAATTTCTGATATTGATGACATAAGAGAAGTTTTAAAAGCAGGAGCTGATAAGGTTTCTATAAATACACAGGCGGT
AAAACAGCCAACACTTATTCGTCAGGCGGCTTTGAGGTTTGGGAGCCAGTGTGTTGTTGTGGCAATTGACGCTAAAAAGA
GACCAGATGGCACAGGCTACAATGTGTATATAAATGGAGGGAGAATAAACACAGGACTTGATGCTGTTGAGTGGGCTAAA
AAGGTGAAAGATTTGGGTGCAGGAGAAATACTTTTAACAAGTATGGATAAAGACGGGACAAAGGATGGATATGATATTGA
ACTTACAAGGCTTATAAGTGAAGCTGTGAGTATTCCAGTTATAGCATCAGGAGGAGCAGGGAAACCCGAGCATTTTAAAG
AGGTGTTTACACAAGGAAAAGCTGATGCAGCTTTGGCTGCTTCTGTATTTCACTATGGAGAGCTTGATATAAAAGAGTTG
AAAAGATATTTAAAGGATGAGGGTATACCGGTAAGATTGTAG

Upstream 100 bases:

>100_bases
AGATATGGGAGTTACTGGTGCTATTATTGGGAAGGCCTTGTACACTGGTATGATAGACTTAAAAGCTGCATTGCTGGAAT
TAGAAAGAGAGGGAATTTAA

Downstream 100 bases:

>100_bases
GAGAAAGGATGTAGAGGATGATTGACATAAAAGAGCTTAAGTTTGACGAAAAGGGTCTTATACCGGCAATTGTGCAAGAT
TATTACACAAAGCAGGTTTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLAKRIIPCLDVKDGRVVKGINFVNLKDAGDPVEIAERYNELGADELVFLDITASYEKRKIMIDVVKRTSEKVFIPLTVG
GGISDIDDIREVLKAGADKVSINTQAVKQPTLIRQAALRFGSQCVVVAIDAKKRPDGTGYNVYINGGRINTGLDAVEWAK
KVKDLGAGEILLTSMDKDGTKDGYDIELTRLISEAVSIPVIASGGAGKPEHFKEVFTQGKADAALAASVFHYGELDIKEL
KRYLKDEGIPVRL

Sequences:

>Translated_253_residues
MLAKRIIPCLDVKDGRVVKGINFVNLKDAGDPVEIAERYNELGADELVFLDITASYEKRKIMIDVVKRTSEKVFIPLTVG
GGISDIDDIREVLKAGADKVSINTQAVKQPTLIRQAALRFGSQCVVVAIDAKKRPDGTGYNVYINGGRINTGLDAVEWAK
KVKDLGAGEILLTSMDKDGTKDGYDIELTRLISEAVSIPVIASGGAGKPEHFKEVFTQGKADAALAASVFHYGELDIKEL
KRYLKDEGIPVRL
>Mature_253_residues
MLAKRIIPCLDVKDGRVVKGINFVNLKDAGDPVEIAERYNELGADELVFLDITASYEKRKIMIDVVKRTSEKVFIPLTVG
GGISDIDDIREVLKAGADKVSINTQAVKQPTLIRQAALRFGSQCVVVAIDAKKRPDGTGYNVYINGGRINTGLDAVEWAK
KVKDLGAGEILLTSMDKDGTKDGYDIELTRLISEAVSIPVIASGGAGKPEHFKEVFTQGKADAALAASVFHYGELDIKEL
KRYLKDEGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=48.062015503876, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082028, Length=206, Percent_Identity=28.6407766990291, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.2165605095541, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_THETN (Q8R885)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623694.1
- ProteinModelPortal:   Q8R885
- SMR:   Q8R885
- GeneID:   997443
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2133
- NMPDR:   fig|273068.3.peg.2237
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   TTEN273068:TTE2133-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27517; Mature: 27517

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGRVVKGINFVNLKDAGDPVEIAERYNELGADELVFLDITASYEKRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IMIDVVKRTSEKVFIPLTVGGGISDIDDIREVLKAGADKVSINTQAVKQPTLIRQAALRF
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQCVVVAIDAKKRPDGTGYNVYINGGRINTGLDAVEWAKKVKDLGAGEILLTSMDKDGT
CCCEEEEEEECCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIELTRLISEAVSIPVIASGGAGKPEHFKEVFTQGKADAALAASVFHYGELDIKEL
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
KRYLKDEGIPVRL
HHHHHCCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVKDGRVVKGINFVNLKDAGDPVEIAERYNELGADELVFLDITASYEKRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IMIDVVKRTSEKVFIPLTVGGGISDIDDIREVLKAGADKVSINTQAVKQPTLIRQAALRF
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQCVVVAIDAKKRPDGTGYNVYINGGRINTGLDAVEWAKKVKDLGAGEILLTSMDKDGT
CCCEEEEEEECCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIELTRLISEAVSIPVIASGGAGKPEHFKEVFTQGKADAALAASVFHYGELDIKEL
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
KRYLKDEGIPVRL
HHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336