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The map label for this gene is GlpK [H]

Identifier: 20808405

GI number: 20808405

Start: 1951236

End: 1952729

Strand: Reverse

Name: GlpK [H]

Synonym: TTE2002

Alternate gene names: 20808405

Gene position: 1952729-1951236 (Counterclockwise)

Preceding gene: 20808406

Following gene: 20808404

Centisome position: 72.61

GC content: 43.71

Gene sequence:

>1494_bases
ATGGCGAAATATGTAATGGCTTTGGACCAGGGGACGACGAGTTCCCGCGCTATAATTTTTGACCACAGCGGTAAAATGAT
TGCTTCTTTAAACAAAGAGTTTAGGCAGATTTATCCTAAACCCGGTTGGGTTGAGCATGACCCAATGGAAATATGGGAAT
CCCAGATAGAAGTTGCAAAAGGAGTAATAGAGAAGGCAGGGATTAAACCAGAGGATATTGCGGCAATAGGAATTACAAAC
CAGAGGGAGACAACAGTCGTATGGGACAAAAACACAGGGAAACCTATTTACAATGCAATAGTGTGGCAGTGCAGGAGGAC
AGCTCCTATATGCGATGACCTTAAGAACAAAGGCTTTGATAAGAAAATAAGAGAAAAGACTGGCCTTGTTGTAGATGCTT
ATTTCTCGGGTACCAAAGTGAAGTGGATTTTGGACAATGTTGAAGGAGCTCGTGAAAAAGCAGAAAGAGGAGAATTGCTT
TTTGGAAATATTGACACATGGCTTATCTGGAATTTGACAAGGGGCAAAGTGCATGTGACTGACTATTCAAATGCTTCAAG
GACAATGCTTTTCAACATACACGAATTGAAGTGGGATAAGGAGATATTAGAAGAGCTCAATGTGCCAGAGAACATGCTTC
CAGAAGTAAAGCCATCAAGCCATGTCTATGGCTATACGGATAAATCGATCTTTGGAGTAGAAATACCTATTGCAGGAGAT
GCAGGAGACCAGCAGGCTGCTTTATTTGGCCAGGCTTGCTTTAAACCCGGAATGGCAAAGAACACTTACGGTACAGGGTG
CTTTATGCTGATGAACACAGGCGAAAAAGCTGTTCCCTCCAAGACCGGGCTTTTGACCACAATAGCTTGGGGTATTGATG
GAAAAGTAGAGTATGCCTTAGAAGGAAGTATTTTCATAACAGGTGCAGCTATTCAGTGGTTGAGGGATGAGCTCAGAATA
ATTGACAATGCACCTCAAAGTGAGGAATACGCCTTAAAGGTTGAAGATACAAATGGAGTGTACGTAGTTCCTGCTTTTGT
GGGTCTAGGTGCTCCTTACTGGGATATGTACGCTAGAGGTGTTATAGTTGGCCTTACAAGGGGAGCAAAGAGAGAGCACA
TAATAAGGGCAACATTGGAGTCTATTGCTTACCAGACGAGAGACGTTCTAGAAGCAATGCAGGAGGATTCCGGAATAAAG
CTTCAGGCTTTGAAGGTTGATGGAGGAGCAAGTGCAAATAACTTCTTAATGCAGTTCCAGGCAGACATTTTAGGCGTTCC
GGTAGATAGGCCTCAGGTGATAGAGACTACTGCATTAGGTGCTGCATACCTTGCAGGGCTGGCGGTGGGCTTCTGGAACA
GCAGAGAAGAAATAGAGAAAAATTGGAATATAGACAGGCGCTTTGAACCTGCCATGGAGGAAGAAAAGAGGGAGAAACTT
TACAGAGGCTGGAAAAAAGCGGTTGAAAGAGCTATGAAGTGGGCTGAAGAATAA

Upstream 100 bases:

>100_bases
TCAGCTTATCATGAGCGTACATTAAAGAGAATTATATCGGCGAGAAGTTCCTCTCGCCGATATGGCATTATAATAAAAAC
TCAAGTGAGGAGGAAGGGAA

Downstream 100 bases:

>100_bases
AAAAATTGATAAAAAGTAGCAGGAATTTAAAAAGTTTTGTAGAATATATCCTAATAATATAGGAAAAAATAAATAATAGG
TATGGTGGAGATTGGGAGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAKYVMALDQGTTSSRAIIFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAKGVIEKAGIKPEDIAAIGITN
QRETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFDKKIREKTGLVVDAYFSGTKVKWILDNVEGAREKAERGELL
FGNIDTWLIWNLTRGKVHVTDYSNASRTMLFNIHELKWDKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGD
AGDQQAALFGQACFKPGMAKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYALEGSIFITGAAIQWLRDELRI
IDNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARGVIVGLTRGAKREHIIRATLESIAYQTRDVLEAMQEDSGIK
LQALKVDGGASANNFLMQFQADILGVPVDRPQVIETTALGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKL
YRGWKKAVERAMKWAEE

Sequences:

>Translated_497_residues
MAKYVMALDQGTTSSRAIIFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAKGVIEKAGIKPEDIAAIGITN
QRETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFDKKIREKTGLVVDAYFSGTKVKWILDNVEGAREKAERGELL
FGNIDTWLIWNLTRGKVHVTDYSNASRTMLFNIHELKWDKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGD
AGDQQAALFGQACFKPGMAKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYALEGSIFITGAAIQWLRDELRI
IDNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARGVIVGLTRGAKREHIIRATLESIAYQTRDVLEAMQEDSGIK
LQALKVDGGASANNFLMQFQADILGVPVDRPQVIETTALGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKL
YRGWKKAVERAMKWAEE
>Mature_496_residues
AKYVMALDQGTTSSRAIIFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAKGVIEKAGIKPEDIAAIGITNQ
RETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFDKKIREKTGLVVDAYFSGTKVKWILDNVEGAREKAERGELLF
GNIDTWLIWNLTRGKVHVTDYSNASRTMLFNIHELKWDKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGDA
GDQQAALFGQACFKPGMAKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYALEGSIFITGAAIQWLRDELRII
DNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARGVIVGLTRGAKREHIIRATLESIAYQTRDVLEAMQEDSGIKL
QALKVDGGASANNFLMQFQADILGVPVDRPQVIETTALGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKLY
RGWKKAVERAMKWAEE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=507, Percent_Identity=47.534516765286, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4504007, Length=507, Percent_Identity=47.534516765286, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI42794763, Length=512, Percent_Identity=47.265625, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=47.3161033797217, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=35.8974358974359, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI164663828, Length=159, Percent_Identity=32.0754716981132, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=68.75, Blast_Score=715, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=502, Percent_Identity=26.8924302788845, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790005, Length=486, Percent_Identity=23.0452674897119, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=23.8383838383838, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789987, Length=497, Percent_Identity=22.5352112676056, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789138, Length=268, Percent_Identity=23.5074626865672, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=42.7435387673956, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71986140, Length=517, Percent_Identity=27.0793036750484, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71986147, Length=271, Percent_Identity=32.4723247232472, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321755, Length=550, Percent_Identity=37.2727272727273, Blast_Score=326, Evalue=5e-90,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=44.7524752475248, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=41.6334661354582, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=41.6334661354582, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=41.6334661354582, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=41.6334661354582, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=41.6334661354582, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24654573, Length=459, Percent_Identity=44.880174291939, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI19922058, Length=528, Percent_Identity=34.0909090909091, Blast_Score=297, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=34.6153846153846, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=28.3495145631068, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=27.2211720226843, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24656372, Length=361, Percent_Identity=30.1939058171745, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45552909, Length=302, Percent_Identity=28.476821192053, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55760; Mature: 55629

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYVMALDQGTTSSRAIIFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAK
CCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GVIEKAGIKPEDIAAIGITNQRETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFD
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCC
KKIREKTGLVVDAYFSGTKVKWILDNVEGAREKAERGELLFGNIDTWLIWNLTRGKVHVT
HHHHHHCCCEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEECCCEEEEEEEECCEEEEE
DYSNASRTMLFNIHELKWDKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGD
ECCCCCCEEEEEHHHHHCCHHHHHHCCCCHHHCCCCCCCCCEEECCCCCEEEEEEEECCC
AGDQQAALFGQACFKPGMAKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYAL
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
EGSIFITGAAIQWLRDELRIIDNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARG
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHCCCCHHHHHHCC
VIVGLTRGAKREHIIRATLESIAYQTRDVLEAMQEDSGIKLQALKVDGGASANNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHEEEE
ADILGVPVDRPQVIETTALGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
YRGWKKAVERAMKWAEE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKYVMALDQGTTSSRAIIFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAK
CCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GVIEKAGIKPEDIAAIGITNQRETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFD
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCC
KKIREKTGLVVDAYFSGTKVKWILDNVEGAREKAERGELLFGNIDTWLIWNLTRGKVHVT
HHHHHHCCCEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEECCCEEEEEEEECCEEEEE
DYSNASRTMLFNIHELKWDKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGD
ECCCCCCEEEEEHHHHHCCHHHHHHCCCCHHHCCCCCCCCCEEECCCCCEEEEEEEECCC
AGDQQAALFGQACFKPGMAKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYAL
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
EGSIFITGAAIQWLRDELRIIDNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARG
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHCCCCHHHHHHCC
VIVGLTRGAKREHIIRATLESIAYQTRDVLEAMQEDSGIKLQALKVDGGASANNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHEEEE
ADILGVPVDRPQVIETTALGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
YRGWKKAVERAMKWAEE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA