Click here to switch to the map view.

The map label for this gene is GlpF [H]

Identifier: 20808406

GI number: 20808406

Start: 1952805

End: 1953533

Strand: Reverse

Name: GlpF [H]

Synonym: TTE2003

Alternate gene names: 20808406

Gene position: 1953533-1952805 (Counterclockwise)

Preceding gene: 20808407

Following gene: 20808405

Centisome position: 72.64

GC content: 41.15

Gene sequence:

>729_bases
ATGAGTGACTTTGCAAAGTATTTGGCTGAATTTTTTGGTACTATGATACTTATTTGGCTAGGTGACGGTGTAGTTGCAAA
CGTTGTCTTAAATAAATCTAAAGGTCAAAATAGCGGCTGGATTGTAATCACCGCAGGATGGGGATTTGCAGTTATGGTAG
GAGCGTATACAGTTGGCTGGCTAAGTGGTGCTCACTTAAATCCAGCTGTGACAATTGGCTTAGCAACGATAGGCAAGTTT
TCGTGGAGTTTAGTGCCAGGCTACATTATAGCACAAGTTTTAGGAGCTTTTGTGGGAGCTATAATTGTTTATTTGATGTA
TATAGATCATTTTGCAGCAACTGAAGATCCCACAGCTAAGCTTGGTACTTTTGCAACTATTCCAGCTATAAGAAATTTAG
GTAAGAATTTTATAACTGAGGCTTTTGGTACTGCCATGCTTTTAATAGGTATACTTGGCATTACAAATAGTAATAACCCT
ATAGGGGGAATGGGACCCCTTTTAGTAGGACTTCTCGTATGGGCAATAGGTTTAAGCTTGGGTGGTCCTACAGGGTATGC
TATTAATCCAGCCAGAGACTTCGGACCGAGACTTGCCCACGCTATACTCCCAATTCCGGGCAAGAGAGATTCTGACTGGG
GGTATGGTTTGATAGTTCCGATATTTGGACCAATAGTTGGAGGAATAATTGGAGCCTGGATTTATCAGCTTATCATGAGC
GTACATTAA

Upstream 100 bases:

>100_bases
AGCGAAAAAAGCCTCTGGTTTATAGAGTGAAAGGGGGGATATATGAAAAACAAAAAATTTTATTAAAAGTTAAATTTTGG
AATTATGGAGGGGATATTTT

Downstream 100 bases:

>100_bases
AGAGAATTATATCGGCGAGAAGTTCCTCTCGCCGATATGGCATTATAATAAAAACTCAAGTGAGGAGGAAGGGAAATGGC
GAAATATGTAATGGCTTTGG

Product: glycerol uptake facilitator and related permeases (major Intrinsic protein family)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSDFAKYLAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVGAYTVGWLSGAHLNPAVTIGLATIGKF
SWSLVPGYIIAQVLGAFVGAIIVYLMYIDHFAATEDPTAKLGTFATIPAIRNLGKNFITEAFGTAMLLIGILGITNSNNP
IGGMGPLLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAILPIPGKRDSDWGYGLIVPIFGPIVGGIIGAWIYQLIMS
VH

Sequences:

>Translated_242_residues
MSDFAKYLAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVGAYTVGWLSGAHLNPAVTIGLATIGKF
SWSLVPGYIIAQVLGAFVGAIIVYLMYIDHFAATEDPTAKLGTFATIPAIRNLGKNFITEAFGTAMLLIGILGITNSNNP
IGGMGPLLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAILPIPGKRDSDWGYGLIVPIFGPIVGGIIGAWIYQLIMS
VH
>Mature_241_residues
SDFAKYLAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVGAYTVGWLSGAHLNPAVTIGLATIGKFS
WSLVPGYIIAQVLGAFVGAIIVYLMYIDHFAATEDPTAKLGTFATIPAIRNLGKNFITEAFGTAMLLIGILGITNSNNPI
GGMGPLLVGLLVWAIGLSLGGPTGYAINPARDFGPRLAHAILPIPGKRDSDWGYGLIVPIFGPIVGGIIGAWIYQLIMSV
H

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=255, Percent_Identity=40.7843137254902, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI157266307, Length=262, Percent_Identity=38.9312977099237, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI4502187, Length=248, Percent_Identity=37.0967741935484, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI22538420, Length=257, Percent_Identity=33.852140077821, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=37.5690607734807, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=37.5690607734807, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37694062, Length=257, Percent_Identity=31.5175097276265, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790362, Length=255, Percent_Identity=38.8235294117647, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787101, Length=192, Percent_Identity=33.8541666666667, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994009, Length=244, Percent_Identity=35.655737704918, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=36.5461847389558, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=35.1020408163265, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=35.1020408163265, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=34.8360655737705, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=34.8360655737705, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=34.0080971659919, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=238, Percent_Identity=32.3529411764706, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=243, Percent_Identity=27.5720164609054, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71993722, Length=240, Percent_Identity=27.5, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=199, Percent_Identity=38.6934673366834, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322985, Length=283, Percent_Identity=28.9752650176678, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652747, Length=237, Percent_Identity=31.2236286919831, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45551084, Length=237, Percent_Identity=31.2236286919831, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550503, Length=234, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25460; Mature: 25329

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFAKYLAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVGAYTVGW
CCHHHHHHHHHHHHHEEHHHCCCCEEHEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHH
LSGAHLNPAVTIGLATIGKFSWSLVPGYIIAQVLGAFVGAIIVYLMYIDHFAATEDPTAK
HCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LGTFATIPAIRNLGKNFITEAFGTAMLLIGILGITNSNNPIGGMGPLLVGLLVWAIGLSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
GGPTGYAINPARDFGPRLAHAILPIPGKRDSDWGYGLIVPIFGPIVGGIIGAWIYQLIMS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
VH
CC
>Mature Secondary Structure 
SDFAKYLAEFFGTMILIWLGDGVVANVVLNKSKGQNSGWIVITAGWGFAVMVGAYTVGW
CHHHHHHHHHHHHHEEHHHCCCCEEHEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHH
LSGAHLNPAVTIGLATIGKFSWSLVPGYIIAQVLGAFVGAIIVYLMYIDHFAATEDPTAK
HCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LGTFATIPAIRNLGKNFITEAFGTAMLLIGILGITNSNNPIGGMGPLLVGLLVWAIGLSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
GGPTGYAINPARDFGPRLAHAILPIPGKRDSDWGYGLIVPIFGPIVGGIIGAWIYQLIMS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
VH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]