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The map label for this gene is PspF5 [C]

Identifier: 20808135

GI number: 20808135

Start: 1659776

End: 1661608

Strand: Reverse

Name: PspF5 [C]

Synonym: TTE1710

Alternate gene names: 20808135

Gene position: 1661608-1659776 (Counterclockwise)

Preceding gene: 20808136

Following gene: 20808134

Centisome position: 61.78

GC content: 37.53

Gene sequence:

>1833_bases
ATGTTAATCAATACCTCGAAGCACGAAGTTTATTATCAGGCAGGTTACTCTATTGGCAGTCAACTTCTTGTAAATGCCCT
AGAAGAAATACCTGCTAAAATAGGCGAACTTGGAATGGGAGACCTTAAGCTGCAAAGTGCAAAAAATGATAAGATAATAG
TCAAATGGCATGAGTGTTTAACATGTTCGCACTTGCTTCCTATAGGTGAGCCTCAATGCTATTTAGAAACGGGTTTGTTA
GCGGGGGTTCTCAACAAACTTTTTAAAAAGAAAGTGGAAGTAAAAGAGACTAAGTGTTGGGGGACAGGAGAAAAGTATTG
TGAGATGGAAGCTACCATCTATCCTACTTCTACCAGTGAGGGTTTTAATTTCGTTTTTTCAGAAGAAAGCAATAAATTAC
TTTTAGCGCTTGCTGTCCAATCTGTTCAAGCAAATAAAAACTATCAAAAGGTCCTCTTAGGGTATATGAACGACGTGAAA
GAATTATCTTTAAGCAACGTGGAAACCCTCTTCCATATGATGCCTTTTGGTCTGATAATTGTTGATAGTCAGGGGAAAGT
AGTAGAAATAAATAAAACTAGTGTTCATATGCTAGGCATTGGTGGACAAAGGGTGCAAGGTCGATTGTTAGAAGAGATAA
TACCTTTAACTAATTATTCTGAAATACTTACGGCGGGTAAACCTCAAGTATGGACCTTTCAAAGCACAAAAGGGGAAACT
ATTATTGTGATAGGCATGCCTTTTAGTGCCCGTGTTAAAAATGAATACATGCTGTTTCAGCTTTTACCTGCTGAAAGAGA
ACTGGTATATCATCTTTTGGAATCCATTACAAAGTCAGAAATAGGAATTGAATATAACTATAAAGTTAGAGAAGTCAATT
GCTCTAAACCTTTTCAATTTAATCATATTCGAACTTTAAATTGGTGTTTTAAAAACGTTTTAATTTTAGCCCAAAAAGCA
GCTAAAACTAATGCTACCATCTTAATTGTAGGAGAGAGTGGTACAGGAAAAGGAGTGTTAGCACAGGCTATTCATCAGGA
AAGTTTTCGCCGCCATGGTCCTTTTATAAGAGTGAATTGTGCTGCTATCCCTTCTGAACTGTTGGAATCTGAACTTTTTG
GGTATGAAGAAGGTGCTTTTACTGGAGCTAAGAGGGGCGGAAAGCCTGGTAAATTTGAATTGGCAGATGGAGGGACTATT
TTTTTAGATGAAATTGGAGATATGCCTCTTAGCATGCAGGCAAAACTTTTAACTGTCTTGCAAGAAAAAGTGGTAGAAAG
ATTGGGAAGTACTAAGGCAAGAAAAATAGATGTGCGAGTGATAGCAGCGACTAGTAGAAATTTGGAAAAAATGGTGCAAG
AAGGATGTTTCAGGGAAGACCTTTTTTATCGCTTAAACGTAGTTAAGCTGTTACTTCCTCCTTTACGAGAAAGGCCAGAA
GACATACCGTTCTTGGTGGAAGATATTTTAAAGAGAATAAACGAGGAATGTGGAAAAAAATTGCGGATTTCTCCTGCAGC
TATGGAATATCTTTTAAGCTATTCTTGGCCCGGCAATGTGAGGGAATTGGAAAATGTTTTAGAGAGGGCTGCTATATTGG
CTGAGGGCCAGGAAATTCAGCCTCAACATTTAATGATAGACTATTTTGGACAAAATAAAAGTATAAAGAAAATTTCACCA
CTTTGTGAAGTCCAAATGGAAGCAGAAAAGGAAGCTATTATTCGAGCTTTAGAAGCTTTTGGTGGCGAAAAATCACGTGC
TGCTAAAGCATTAGGTATCTCTCGGCAAGCTCTTTATGCTAAAATGGTTCGCTATGGATTGCTTAAAAAATAG

Upstream 100 bases:

>100_bases
GATTCAGTTGAGGAGCTAAAAAGAAACTATGATACTTATTTAGCATATGTGAGAAGTTTTTGAATGATTAACAATAAGTA
CAATTAAAGGGGGAAGAGGT

Downstream 100 bases:

>100_bases
TGTCAATTTTTTTTGACGTGTAAATTTAAATTGACATGATAGATAACAAGACCGGTTGATTTTAACGGTCTTTTTCTTTT
GGCACGATTTTTGCTAAACA

Product: transcriptional regulator

Products: NA

Alternate protein names: (S)-limonene 6-monooxygenase; (S)-limonene 7-monooxygenase; Carveol dehydrogenase; Perillyl-alcohol dehydrogenase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MLINTSKHEVYYQAGYSIGSQLLVNALEEIPAKIGELGMGDLKLQSAKNDKIIVKWHECLTCSHLLPIGEPQCYLETGLL
AGVLNKLFKKKVEVKETKCWGTGEKYCEMEATIYPTSTSEGFNFVFSEESNKLLLALAVQSVQANKNYQKVLLGYMNDVK
ELSLSNVETLFHMMPFGLIIVDSQGKVVEINKTSVHMLGIGGQRVQGRLLEEIIPLTNYSEILTAGKPQVWTFQSTKGET
IIVIGMPFSARVKNEYMLFQLLPAERELVYHLLESITKSEIGIEYNYKVREVNCSKPFQFNHIRTLNWCFKNVLILAQKA
AKTNATILIVGESGTGKGVLAQAIHQESFRRHGPFIRVNCAAIPSELLESELFGYEEGAFTGAKRGGKPGKFELADGGTI
FLDEIGDMPLSMQAKLLTVLQEKVVERLGSTKARKIDVRVIAATSRNLEKMVQEGCFREDLFYRLNVVKLLLPPLRERPE
DIPFLVEDILKRINEECGKKLRISPAAMEYLLSYSWPGNVRELENVLERAAILAEGQEIQPQHLMIDYFGQNKSIKKISP
LCEVQMEAEKEAIIRALEAFGGEKSRAAKALGISRQALYAKMVRYGLLKK

Sequences:

>Translated_610_residues
MLINTSKHEVYYQAGYSIGSQLLVNALEEIPAKIGELGMGDLKLQSAKNDKIIVKWHECLTCSHLLPIGEPQCYLETGLL
AGVLNKLFKKKVEVKETKCWGTGEKYCEMEATIYPTSTSEGFNFVFSEESNKLLLALAVQSVQANKNYQKVLLGYMNDVK
ELSLSNVETLFHMMPFGLIIVDSQGKVVEINKTSVHMLGIGGQRVQGRLLEEIIPLTNYSEILTAGKPQVWTFQSTKGET
IIVIGMPFSARVKNEYMLFQLLPAERELVYHLLESITKSEIGIEYNYKVREVNCSKPFQFNHIRTLNWCFKNVLILAQKA
AKTNATILIVGESGTGKGVLAQAIHQESFRRHGPFIRVNCAAIPSELLESELFGYEEGAFTGAKRGGKPGKFELADGGTI
FLDEIGDMPLSMQAKLLTVLQEKVVERLGSTKARKIDVRVIAATSRNLEKMVQEGCFREDLFYRLNVVKLLLPPLRERPE
DIPFLVEDILKRINEECGKKLRISPAAMEYLLSYSWPGNVRELENVLERAAILAEGQEIQPQHLMIDYFGQNKSIKKISP
LCEVQMEAEKEAIIRALEAFGGEKSRAAKALGISRQALYAKMVRYGLLKK
>Mature_610_residues
MLINTSKHEVYYQAGYSIGSQLLVNALEEIPAKIGELGMGDLKLQSAKNDKIIVKWHECLTCSHLLPIGEPQCYLETGLL
AGVLNKLFKKKVEVKETKCWGTGEKYCEMEATIYPTSTSEGFNFVFSEESNKLLLALAVQSVQANKNYQKVLLGYMNDVK
ELSLSNVETLFHMMPFGLIIVDSQGKVVEINKTSVHMLGIGGQRVQGRLLEEIIPLTNYSEILTAGKPQVWTFQSTKGET
IIVIGMPFSARVKNEYMLFQLLPAERELVYHLLESITKSEIGIEYNYKVREVNCSKPFQFNHIRTLNWCFKNVLILAQKA
AKTNATILIVGESGTGKGVLAQAIHQESFRRHGPFIRVNCAAIPSELLESELFGYEEGAFTGAKRGGKPGKFELADGGTI
FLDEIGDMPLSMQAKLLTVLQEKVVERLGSTKARKIDVRVIAATSRNLEKMVQEGCFREDLFYRLNVVKLLLPPLRERPE
DIPFLVEDILKRINEECGKKLRISPAAMEYLLSYSWPGNVRELENVLERAAILAEGQEIQPQHLMIDYFGQNKSIKKISP
LCEVQMEAEKEAIIRALEAFGGEKSRAAKALGISRQALYAKMVRYGLLKK

Specific function: Involved in limonene hydroxylation to a mixture of carveol and perillyl alcohol as well as in dehydrogenation of these products to carvone and perillyl aldehyde. Aromatic alcohols containing an isopropyl or isopropenyl group at ring position 4 also served

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=324, Percent_Identity=47.5308641975309, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI87082117, Length=301, Percent_Identity=46.5116279069767, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1790437, Length=291, Percent_Identity=49.1408934707904, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1789087, Length=302, Percent_Identity=46.0264900662252, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI1786524, Length=301, Percent_Identity=47.5083056478405, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1790299, Length=324, Percent_Identity=42.9012345679012, Blast_Score=245, Evalue=7e-66,
Organism=Escherichia coli, GI1789233, Length=455, Percent_Identity=34.5054945054945, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1788905, Length=292, Percent_Identity=44.1780821917808, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787583, Length=442, Percent_Identity=33.4841628959276, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=39.297124600639, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=286, Percent_Identity=41.958041958042, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081858, Length=452, Percent_Identity=29.646017699115, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=37.6518218623482, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: =1.14.13.48; =1.14.13.49; =1.1.1.243; =1.1.1.144 [H]

Molecular weight: Translated: 68468; Mature: 68468

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINTSKHEVYYQAGYSIGSQLLVNALEEIPAKIGELGMGDLKLQSAKNDKIIVKWHECL
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHHH
TCSHLLPIGEPQCYLETGLLAGVLNKLFKKKVEVKETKCWGTGEKYCEMEATIYPTSTSE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCC
GFNFVFSEESNKLLLALAVQSVQANKNYQKVLLGYMNDVKELSLSNVETLFHMMPFGLII
CCEEEEECCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDSQGKVVEINKTSVHMLGIGGQRVQGRLLEEIIPLTNYSEILTAGKPQVWTFQSTKGET
ECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCE
IIVIGMPFSARVKNEYMLFQLLPAERELVYHLLESITKSEIGIEYNYKVREVNCSKPFQF
EEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCC
NHIRTLNWCFKNVLILAQKAAKTNATILIVGESGTGKGVLAQAIHQESFRRHGPFIRVNC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEH
AAIPSELLESELFGYEEGAFTGAKRGGKPGKFELADGGTIFLDEIGDMPLSMQAKLLTVL
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHH
QEKVVERLGSTKARKIDVRVIAATSRNLEKMVQEGCFREDLFYRLNVVKLLLPPLRERPE
HHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCC
DIPFLVEDILKRINEECGKKLRISPAAMEYLLSYSWPGNVRELENVLERAAILAEGQEIQ
CCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
PQHLMIDYFGQNKSIKKISPLCEVQMEAEKEAIIRALEAFGGEKSRAAKALGISRQALYA
CCEEEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHH
KMVRYGLLKK
HHHHHHCCCC
>Mature Secondary Structure
MLINTSKHEVYYQAGYSIGSQLLVNALEEIPAKIGELGMGDLKLQSAKNDKIIVKWHECL
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHHH
TCSHLLPIGEPQCYLETGLLAGVLNKLFKKKVEVKETKCWGTGEKYCEMEATIYPTSTSE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCC
GFNFVFSEESNKLLLALAVQSVQANKNYQKVLLGYMNDVKELSLSNVETLFHMMPFGLII
CCEEEEECCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDSQGKVVEINKTSVHMLGIGGQRVQGRLLEEIIPLTNYSEILTAGKPQVWTFQSTKGET
ECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCE
IIVIGMPFSARVKNEYMLFQLLPAERELVYHLLESITKSEIGIEYNYKVREVNCSKPFQF
EEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCC
NHIRTLNWCFKNVLILAQKAAKTNATILIVGESGTGKGVLAQAIHQESFRRHGPFIRVNC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEH
AAIPSELLESELFGYEEGAFTGAKRGGKPGKFELADGGTIFLDEIGDMPLSMQAKLLTVL
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHH
QEKVVERLGSTKARKIDVRVIAATSRNLEKMVQEGCFREDLFYRLNVVKLLLPPLRERPE
HHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCC
DIPFLVEDILKRINEECGKKLRISPAAMEYLLSYSWPGNVRELENVLERAAILAEGQEIQ
CCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
PQHLMIDYFGQNKSIKKISPLCEVQMEAEKEAIIRALEAFGGEKSRAAKALGISRQALYA
CCEEEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHH
KMVRYGLLKK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA