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The map label for this gene is NuoI2 [H]

Identifier: 20808125

GI number: 20808125

Start: 1647026

End: 1647622

Strand: Reverse

Name: NuoI2 [H]

Synonym: TTE1699

Alternate gene names: 20808125

Gene position: 1647622-1647026 (Counterclockwise)

Preceding gene: 20808126

Following gene: 20808124

Centisome position: 61.26

GC content: 40.7

Gene sequence:

>597_bases
ATGTTAAGACTGCTTAAAAAGGCGCTGCAGGTTGGAGAAGCAACAGTTGAGTATCCTTTTAAGCCGGTAGAAGTAGCTCA
AGGTTTTCGCGGGAAGCCGGCGTATGACTTCAGCCGGTGTATAGGTTGTGGGGCTTGTGCTACTGCTTGTCCTTCAAATG
CTATTACCATGGATTGTGATCTAGATAGAGGGATTAAGTCCTGGAATATAAATTACGGCCGCTGCATTTTTTGCGGTCGT
TGTGAAGAGGTGTGTCCTACAGGAGCTATTGTACTTTCAACAGAATTTGAACTGGCTGTTATTAAAAAAGAGGATATGTA
CTGTCGAGCTGAGTTTCAGCTTTGTAGATGTATCTTTTGTGGGGAATACTTTGAAACATTTCGTGAAATAGAATACGTAC
GAGAAATATTAGAACAAACAGGTTTGACAAAGAAGAGCTTGGAAAGTTGGGTTCAACTTCTTAAAGTATGTCCAAAATGT
AGGCGTCATAAAGTAGCAAGGATAGCAGCACTAGATAATTGGCATGTTGATTTAAATGGGAGGAGGGAGGAAAAATGGAA
CCAGAAGCTAAGTTACAAGAACTTAAAGCAAAGTTGA

Upstream 100 bases:

>100_bases
AATATCAAGAATTGGAACAATACTGTCGCGAAAGAAAATATTCACCACTGAAAAATTAGCTTAACTATAACTATAAAAGC
AAGGAAGGGGATATGAAAAG

Downstream 100 bases:

>100_bases
AAAATGTCATTAAACGTTCTGCCTACCTCTACAGGGTAGACTGTGGCGGATGTAATGGATGCGAGATTGAAATTTTCGCT
GCTATCTCACCTGTTTATGA

Product: formate hydrogenlyase complex iron-sulfur subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MLRLLKKALQVGEATVEYPFKPVEVAQGFRGKPAYDFSRCIGCGACATACPSNAITMDCDLDRGIKSWNINYGRCIFCGR
CEEVCPTGAIVLSTEFELAVIKKEDMYCRAEFQLCRCIFCGEYFETFREIEYVREILEQTGLTKKSLESWVQLLKVCPKC
RRHKVARIAALDNWHVDLNGRREEKWNQKLSYKNLKQS

Sequences:

>Translated_198_residues
MLRLLKKALQVGEATVEYPFKPVEVAQGFRGKPAYDFSRCIGCGACATACPSNAITMDCDLDRGIKSWNINYGRCIFCGR
CEEVCPTGAIVLSTEFELAVIKKEDMYCRAEFQLCRCIFCGEYFETFREIEYVREILEQTGLTKKSLESWVQLLKVCPKC
RRHKVARIAALDNWHVDLNGRREEKWNQKLSYKNLKQS
>Mature_198_residues
MLRLLKKALQVGEATVEYPFKPVEVAQGFRGKPAYDFSRCIGCGACATACPSNAITMDCDLDRGIKSWNINYGRCIFCGR
CEEVCPTGAIVLSTEFELAVIKKEDMYCRAEFQLCRCIFCGEYFETFREIEYVREILEQTGLTKKSLESWVQLLKVCPKC
RRHKVARIAALDNWHVDLNGRREEKWNQKLSYKNLKQS

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=102, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789075, Length=164, Percent_Identity=46.9512195121951, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788833, Length=164, Percent_Identity=48.780487804878, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788617, Length=101, Percent_Identity=37.6237623762376, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17555194, Length=102, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864306, Length=111, Percent_Identity=35.1351351351351, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 22790; Mature: 22790

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLKKALQVGEATVEYPFKPVEVAQGFRGKPAYDFSRCIGCGACATACPSNAITMDCD
CHHHHHHHHHHCCCHHCCCCCHHHHHCCCCCCCCCHHHHHHCCCHHHHHCCCCCEEEECC
LDRGIKSWNINYGRCIFCGRCEEVCPTGAIVLSTEFELAVIKKEDMYCRAEFQLCRCIFC
CCCCCCCCCCCCCEEEEECCCHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
GEYFETFREIEYVREILEQTGLTKKSLESWVQLLKVCPKCRRHKVARIAALDNWHVDLNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCC
RREEKWNQKLSYKNLKQS
CCHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MLRLLKKALQVGEATVEYPFKPVEVAQGFRGKPAYDFSRCIGCGACATACPSNAITMDCD
CHHHHHHHHHHCCCHHCCCCCHHHHHCCCCCCCCCHHHHHHCCCHHHHHCCCCCEEEECC
LDRGIKSWNINYGRCIFCGRCEEVCPTGAIVLSTEFELAVIKKEDMYCRAEFQLCRCIFC
CCCCCCCCCCCCCEEEEECCCHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
GEYFETFREIEYVREILEQTGLTKKSLESWVQLLKVCPKCRRHKVARIAALDNWHVDLNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCC
RREEKWNQKLSYKNLKQS
CCHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]