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The map label for this gene is hycG [H]

Identifier: 20808124

GI number: 20808124

Start: 1646325

End: 1647068

Strand: Reverse

Name: hycG [H]

Synonym: TTE1698

Alternate gene names: 20808124

Gene position: 1647068-1646325 (Counterclockwise)

Preceding gene: 20808125

Following gene: 20808123

Centisome position: 61.24

GC content: 45.16

Gene sequence:

>744_bases
ATGGAACCAGAAGCTAAGTTACAAGAACTTAAAGCAAAGTTGAAAAATGTCATTAAACGTTCTGCCTACCTCTACAGGGT
AGACTGTGGCGGATGTAATGGATGCGAGATTGAAATTTTCGCTGCTATCTCACCTGTTTATGACCCAGAAAGGTTTGGCA
TCAAAATAGTGTCCTCGCCGCGGCATGCTGATATTCTTCTCTTTACCGGACCTATGACAAGGGCTATGCGGCTTCCTGCT
ATACGGGCGTATAAAGCAGCACCTGACCCTAAAATCGTAGTGGCTTACGGCGCTTGTGGCTGCAGTGGCGGTATTTTTCA
CGACTGCTATTGTGTATGGGGAGGAATTGACAAATTCTTTCCCGTAGATGTATATATCCCCGGCTGTCCTCCAACTCCGG
CTGCTACTTTGTACGGCTTAGCTGTGGCTTTGGATTTGCTACATCAGAAAATTAAAGGAGAGCAGTTTGTAGAAACAGAG
GTTACCCCTGTTCCTGCAAAATATGAGGGGGTTTGCCCTGACCTAGCTAAGGATATTGAAAGGGAAGCCCGACGCCTGAG
CGGTTACTTGCATGGTAGGCGGATAGCTGCACAATATCTGGAATTTGTGAAACAAGGAGACCCGCTGTCAGTAGATCGCA
AAATTAAAGAGTTGGTAAAACAACAAAAAGATCCACGATTGGCTGAAATTTTTTTAAGTTTGCACAAAATTTACATGGAA
AGGGTAGGTCATGATGCCGGGTAA

Upstream 100 bases:

>100_bases
AACTTCTTAAAGTATGTCCAAAATGTAGGCGTCATAAAGTAGCAAGGATAGCAGCACTAGATAATTGGCATGTTGATTTA
AATGGGAGGAGGGAGGAAAA

Downstream 100 bases:

>100_bases
AGTAAAATTCTTTAAACTCTCCAAAAGGTTTGTGGACGAAAAAGATGCCCCTGCCGAAGTCGAACAGCTAAAATACTATG
CTTTAGCTATTGGTCACCAT

Product: Ni,Fe-hydrogenase III small subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MEPEAKLQELKAKLKNVIKRSAYLYRVDCGGCNGCEIEIFAAISPVYDPERFGIKIVSSPRHADILLFTGPMTRAMRLPA
IRAYKAAPDPKIVVAYGACGCSGGIFHDCYCVWGGIDKFFPVDVYIPGCPPTPAATLYGLAVALDLLHQKIKGEQFVETE
VTPVPAKYEGVCPDLAKDIEREARRLSGYLHGRRIAAQYLEFVKQGDPLSVDRKIKELVKQQKDPRLAEIFLSLHKIYME
RVGHDAG

Sequences:

>Translated_247_residues
MEPEAKLQELKAKLKNVIKRSAYLYRVDCGGCNGCEIEIFAAISPVYDPERFGIKIVSSPRHADILLFTGPMTRAMRLPA
IRAYKAAPDPKIVVAYGACGCSGGIFHDCYCVWGGIDKFFPVDVYIPGCPPTPAATLYGLAVALDLLHQKIKGEQFVETE
VTPVPAKYEGVCPDLAKDIEREARRLSGYLHGRRIAAQYLEFVKQGDPLSVDRKIKELVKQQKDPRLAEIFLSLHKIYME
RVGHDAG
>Mature_247_residues
MEPEAKLQELKAKLKNVIKRSAYLYRVDCGGCNGCEIEIFAAISPVYDPERFGIKIVSSPRHADILLFTGPMTRAMRLPA
IRAYKAAPDPKIVVAYGACGCSGGIFHDCYCVWGGIDKFFPVDVYIPGCPPTPAATLYGLAVALDLLHQKIKGEQFVETE
VTPVPAKYEGVCPDLAKDIEREARRLSGYLHGRRIAAQYLEFVKQGDPLSVDRKIKELVKQQKDPRLAEIFLSLHKIYME
RVGHDAG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=161, Percent_Identity=37.2670807453416, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789074, Length=240, Percent_Identity=57.5, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1788834, Length=234, Percent_Identity=55.1282051282051, Blast_Score=263, Evalue=9e-72,
Organism=Escherichia coli, GI1788624, Length=160, Percent_Identity=36.875, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=34.8387096774194, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859983, Length=111, Percent_Identity=40.5405405405405, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24642371, Length=111, Percent_Identity=40.5405405405405, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24651058, Length=107, Percent_Identity=42.0560747663551, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 27439; Mature: 27439

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPEAKLQELKAKLKNVIKRSAYLYRVDCGGCNGCEIEIFAAISPVYDPERFGIKIVSSP
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHCEEEECCC
RHADILLFTGPMTRAMRLPAIRAYKAAPDPKIVVAYGACGCSGGIFHDCYCVWGGIDKFF
CCCEEEEEECCHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHEECCCCCCE
PVDVYIPGCPPTPAATLYGLAVALDLLHQKIKGEQFVETEVTPVPAKYEGVCPDLAKDIE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHH
REARRLSGYLHGRRIAAQYLEFVKQGDPLSVDRKIKELVKQQKDPRLAEIFLSLHKIYME
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RVGHDAG
HHCCCCC
>Mature Secondary Structure
MEPEAKLQELKAKLKNVIKRSAYLYRVDCGGCNGCEIEIFAAISPVYDPERFGIKIVSSP
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHCEEEECCC
RHADILLFTGPMTRAMRLPAIRAYKAAPDPKIVVAYGACGCSGGIFHDCYCVWGGIDKFF
CCCEEEEEECCHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHEECCCCCCE
PVDVYIPGCPPTPAATLYGLAVALDLLHQKIKGEQFVETEVTPVPAKYEGVCPDLAKDIE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHH
REARRLSGYLHGRRIAAQYLEFVKQGDPLSVDRKIKELVKQQKDPRLAEIFLSLHKIYME
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RVGHDAG
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]