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The map label for this gene is HycE [H]

Identifier: 20808126

GI number: 20808126

Start: 1647664

End: 1649394

Strand: Reverse

Name: HycE [H]

Synonym: TTE1700

Alternate gene names: 20808126

Gene position: 1649394-1647664 (Counterclockwise)

Preceding gene: 20808127

Following gene: 20808125

Centisome position: 61.33

GC content: 44.48

Gene sequence:

>1731_bases
GTGGAGAATGAAATAAAGGCACAACAAGACAGAAAAAAATATGTTAGGGCTTTGTACTCAAAGTTTGGTGCGAGCATCCT
TGAAGAGAAGTGGCAAACACCGGATCAAGTGACTCTGACAGTAAAGCTAAATATACTCCCAGAAATTGTAGAAGAGGCAT
ATTACGGACAGGGAGGGTGGCTGTCAAATGTTATCGGTAACGATGAGCGCAGCATAAACGGACATTTTGCGGTATACTAT
GTTTTATCCATTGAGGCAGAAAAAGACCCGGATAAAAACCTTTGGCTGACAGTTAAGGCTTTGGTTCCTGCTGACAAACC
AGAGTTTCCTTCGGTAGCGCCTCGTGTGCCGGCAGCTGTATGGTACGAACGGGAGGTGCGGGATATGCTGGGGTTGGAGC
CGGTGGGGCTTCCAGATGCGAGAAGACTTGTGTTGCCCGATGACTGGCCAGATAACCTTTATCCTTTGCGAAAGGATATG
GATTATCGTCTACGCCCTGAGCCGGCAGCGGAAGAAGAAAATTATGAGTTTATCGAAGTAAAAGGAGAAGGTATTGTAGA
AGTACCACTGGGACCGCTTCACATTACTTCTGACGAGCCTGGACATTTCCGACTCTATGTCGACGGCGAATATATCGTTG
ATGCCGACTACCGCCTTTTCTATGTGCATCGAGGCCTTGAAAAACTTGCTGAAAGTCGCATGGACTATAACCAGGTTACT
TTTTTAGCGGAGCGAATTTGTGGAATTTGTGGTTATGCACACAGCGTGGCGTATGCCATGGCAGTAGAAGCAGCCAACAG
AATTGAAGTCCCTCTGCGGGCCCAGTATATTCGAACTATTCTATTAGAGGTAGAGAGGATACACAGCCACCTTTTGAATA
TAGGGTTAGCGGCTCACTTAGTGGGTTTTGATTCGGGCTTTATGCATTTCTTTAGAATAAGAGAAAAGGCTATGCAGATG
GCGGAAATTCTTACAGGTGGTCGAAAAACTTATGGAATGAACCTCATAGGAGGTGTACGACGTGATATTTTAAAAGAGGA
GAAGGTTCAGGCCTTGCGATTAATTTCTGAGATTCGATCTGAACTTAAAGAACTTATCGATATGTTGCTAAATACCCCCA
ACTTTATTTCGCGAACACAGGGAGTTGGGCGTCTGGACCCTAAGGTAGCTCGAAACTTTGGATCGGTTGGCCCCAATCTG
AGAGGTTCTGGATATGGACGAGATACACGAGCGGACCATCCCTATTGTGCCTACAAACATGTATCTTGGAATGTTATCTC
CAAGGAGGGATGTGATGTTTTGTCTAGAGTATTAGTACGAGCGGAAGAGCTTTTTGAGTCTTTAGATATAATTGAAAGAT
GTTTGTCTGATTTGCCTCCTGGTCCTATCCTTCATGAAGGGTTTACCTATACACCTTATACTTTTGCACTTGGATATACG
GAAGCTCCTAGGGGAGAAAATGTCCATTGGGTGATGACAGCTAATCAACATCAGGTGTACCGGTGGCGAGTACGGGCCTC
TACTTATAATAATTGGCCAGTCCTTCGTTATATGTTTTACGGTAATACTGTGGCCGATGCACCTTTGATTGTGGCAAGTA
TTGACCCTTGTTACTCTTGCGCTGAAAGAGTTACATTGGTCGATGTGCGTAAAAAGAGGGCAAAAACTGTCCAATATCAA
GAATTGGAACAATACTGTCGCGAAAGAAAATATTCACCACTGAAAAATTAG

Upstream 100 bases:

>100_bases
GCCTTTCTCATAATTATGACTCTTATATCCCAATACATTGCTTTGGCGCTGTTAAGTTAAAAATTGGGTATGTGTCAGAA
TAAAAAGGAGGTTGAAAAGG

Downstream 100 bases:

>100_bases
CTTAACTATAACTATAAAAGCAAGGAAGGGGATATGAAAAGATGTTAAGACTGCTTAAAAAGGCGCTGCAGGTTGGAGAA
GCAACAGTTGAGTATCCTTT

Product: Ni,Fe-hydrogenase III large subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MENEIKAQQDRKKYVRALYSKFGASILEEKWQTPDQVTLTVKLNILPEIVEEAYYGQGGWLSNVIGNDERSINGHFAVYY
VLSIEAEKDPDKNLWLTVKALVPADKPEFPSVAPRVPAAVWYEREVRDMLGLEPVGLPDARRLVLPDDWPDNLYPLRKDM
DYRLRPEPAAEEENYEFIEVKGEGIVEVPLGPLHITSDEPGHFRLYVDGEYIVDADYRLFYVHRGLEKLAESRMDYNQVT
FLAERICGICGYAHSVAYAMAVEAANRIEVPLRAQYIRTILLEVERIHSHLLNIGLAAHLVGFDSGFMHFFRIREKAMQM
AEILTGGRKTYGMNLIGGVRRDILKEEKVQALRLISEIRSELKELIDMLLNTPNFISRTQGVGRLDPKVARNFGSVGPNL
RGSGYGRDTRADHPYCAYKHVSWNVISKEGCDVLSRVLVRAEELFESLDIIERCLSDLPPGPILHEGFTYTPYTFALGYT
EAPRGENVHWVMTANQHQVYRWRVRASTYNNWPVLRYMFYGNTVADAPLIVASIDPCYSCAERVTLVDVRKKRAKTVQYQ
ELEQYCRERKYSPLKN

Sequences:

>Translated_576_residues
MENEIKAQQDRKKYVRALYSKFGASILEEKWQTPDQVTLTVKLNILPEIVEEAYYGQGGWLSNVIGNDERSINGHFAVYY
VLSIEAEKDPDKNLWLTVKALVPADKPEFPSVAPRVPAAVWYEREVRDMLGLEPVGLPDARRLVLPDDWPDNLYPLRKDM
DYRLRPEPAAEEENYEFIEVKGEGIVEVPLGPLHITSDEPGHFRLYVDGEYIVDADYRLFYVHRGLEKLAESRMDYNQVT
FLAERICGICGYAHSVAYAMAVEAANRIEVPLRAQYIRTILLEVERIHSHLLNIGLAAHLVGFDSGFMHFFRIREKAMQM
AEILTGGRKTYGMNLIGGVRRDILKEEKVQALRLISEIRSELKELIDMLLNTPNFISRTQGVGRLDPKVARNFGSVGPNL
RGSGYGRDTRADHPYCAYKHVSWNVISKEGCDVLSRVLVRAEELFESLDIIERCLSDLPPGPILHEGFTYTPYTFALGYT
EAPRGENVHWVMTANQHQVYRWRVRASTYNNWPVLRYMFYGNTVADAPLIVASIDPCYSCAERVTLVDVRKKRAKTVQYQ
ELEQYCRERKYSPLKN
>Mature_576_residues
MENEIKAQQDRKKYVRALYSKFGASILEEKWQTPDQVTLTVKLNILPEIVEEAYYGQGGWLSNVIGNDERSINGHFAVYY
VLSIEAEKDPDKNLWLTVKALVPADKPEFPSVAPRVPAAVWYEREVRDMLGLEPVGLPDARRLVLPDDWPDNLYPLRKDM
DYRLRPEPAAEEENYEFIEVKGEGIVEVPLGPLHITSDEPGHFRLYVDGEYIVDADYRLFYVHRGLEKLAESRMDYNQVT
FLAERICGICGYAHSVAYAMAVEAANRIEVPLRAQYIRTILLEVERIHSHLLNIGLAAHLVGFDSGFMHFFRIREKAMQM
AEILTGGRKTYGMNLIGGVRRDILKEEKVQALRLISEIRSELKELIDMLLNTPNFISRTQGVGRLDPKVARNFGSVGPNL
RGSGYGRDTRADHPYCAYKHVSWNVISKEGCDVLSRVLVRAEELFESLDIIERCLSDLPPGPILHEGFTYTPYTFALGYT
EAPRGENVHWVMTANQHQVYRWRVRASTYNNWPVLRYMFYGNTVADAPLIVASIDPCYSCAERVTLVDVRKKRAKTVQYQ
ELEQYCRERKYSPLKN

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=385, Percent_Identity=27.7922077922078, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI260898743, Length=375, Percent_Identity=28, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1789076, Length=566, Percent_Identity=63.0742049469965, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=561, Percent_Identity=61.319073083779, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=519, Percent_Identity=27.3603082851638, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17568379, Length=388, Percent_Identity=27.319587628866, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17555284, Length=381, Percent_Identity=26.7716535433071, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=29.3963254593176, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI221459469, Length=383, Percent_Identity=27.6762402088773, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 66093; Mature: 66093

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEIKAQQDRKKYVRALYSKFGASILEEKWQTPDQVTLTVKLNILPEIVEEAYYGQGGW
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCC
LSNVIGNDERSINGHFAVYYVLSIEAEKDPDKNLWLTVKALVPADKPEFPSVAPRVPAAV
HHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHH
WYEREVRDMLGLEPVGLPDARRLVLPDDWPDNLYPLRKDMDYRLRPEPAAEEENYEFIEV
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KGEGIVEVPLGPLHITSDEPGHFRLYVDGEYIVDADYRLFYVHRGLEKLAESRMDYNQVT
CCCCEEECCCCCEEECCCCCCEEEEEECCCEEEECCCEEEHHHHHHHHHHHHHCCHHHHH
FLAERICGICGYAHSVAYAMAVEAANRIEVPLRAQYIRTILLEVERIHSHLLNIGLAAHL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFDSGFMHFFRIREKAMQMAEILTGGRKTYGMNLIGGVRRDILKEEKVQALRLISEIRS
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKELIDMLLNTPNFISRTQGVGRLDPKVARNFGSVGPNLRGSGYGRDTRADHPYCAYKH
HHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
VSWNVISKEGCDVLSRVLVRAEELFESLDIIERCLSDLPPGPILHEGFTYTPYTFALGYT
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
EAPRGENVHWVMTANQHQVYRWRVRASTYNNWPVLRYMFYGNTVADAPLIVASIDPCYSC
CCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHH
AERVTLVDVRKKRAKTVQYQELEQYCRERKYSPLKN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENEIKAQQDRKKYVRALYSKFGASILEEKWQTPDQVTLTVKLNILPEIVEEAYYGQGGW
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCC
LSNVIGNDERSINGHFAVYYVLSIEAEKDPDKNLWLTVKALVPADKPEFPSVAPRVPAAV
HHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHH
WYEREVRDMLGLEPVGLPDARRLVLPDDWPDNLYPLRKDMDYRLRPEPAAEEENYEFIEV
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KGEGIVEVPLGPLHITSDEPGHFRLYVDGEYIVDADYRLFYVHRGLEKLAESRMDYNQVT
CCCCEEECCCCCEEECCCCCCEEEEEECCCEEEECCCEEEHHHHHHHHHHHHHCCHHHHH
FLAERICGICGYAHSVAYAMAVEAANRIEVPLRAQYIRTILLEVERIHSHLLNIGLAAHL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFDSGFMHFFRIREKAMQMAEILTGGRKTYGMNLIGGVRRDILKEEKVQALRLISEIRS
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKELIDMLLNTPNFISRTQGVGRLDPKVARNFGSVGPNLRGSGYGRDTRADHPYCAYKH
HHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
VSWNVISKEGCDVLSRVLVRAEELFESLDIIERCLSDLPPGPILHEGFTYTPYTFALGYT
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
EAPRGENVHWVMTANQHQVYRWRVRASTYNNWPVLRYMFYGNTVADAPLIVASIDPCYSC
CCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEECCHHHHH
AERVTLVDVRKKRAKTVQYQELEQYCRERKYSPLKN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]