Click here to switch to the map view.

The map label for this gene is MiaB [H]

Identifier: 20807443

GI number: 20807443

Start: 967016

End: 968329

Strand: Direct

Name: MiaB [H]

Synonym: TTE0962

Alternate gene names: 20807443

Gene position: 967016-968329 (Clockwise)

Preceding gene: 20807440

Following gene: 20807444

Centisome position: 35.96

GC content: 36.91

Gene sequence:

>1314_bases
GTGGCAAAAGTTGCATTTTACACTTTGGGATGCAAAGTGAATCAATATGAGACAGAAGTCATGGCAGAGCTTTTTAGAAA
AGCCGGATATGAAATTGTAGATTTTGATGAAATAGCAGATGTGTATGTAATAAACACCTGCAGTGTTACTGCGAGAAGTG
ATATGAAGTCGAGGCAGATGATAAGAAAGACGAGGAATAAGAATCCAGATGCAATAGTGGTGGCAGTGGGTTGTTATGTT
CAAGTAAGCCCTGATGAAGTTTTTTCAATGCCTGAAGTGGATATAGTAATAGGCACAAAGGATAAAGATAAAATAGTTGA
TTTGGTGAAAGATTTTGAAAATGAGAAAAAGAAAACTAAGCTAATAGAAAATATAATGAAACAGAGAGATTATGAAGAGT
TTGGTATTACTGGGTACACAGAGAGGACAAGGGCTTATATAAAAATTGAGGATGGCTGCAACCAATACTGCACGTACTGC
ATCATTCCTTATGCCAGAGGGCCAGTTAGGAGCAGAAAGCCAGAAAACATAATAAAAGAGGTCAAGAAGTACGCAGAACA
TGGGTATAAAGAAATAGTTCTCACAGGTATACACATTGCTTCTTACGGCAGGGACCTTAAAAATATAGGGCTTTTGGATG
TAATAAAAATGGTTCACGAAGTAGAAGGAATAGAAAGGATAAGGATAAGTTCTATTGAACCCACCTTTTTGACAGAAGAT
GTGGTAAAAGAATTAGCAAATCTTCCTAAAATGTGCAGGCATTATCACGTGTCTCTTCAGAGCGGATGTGATGAAACGTT
GAAAAGAATGGGAAGAAAATACACAGCTTCTGAGTATAAAGAAGTGGTGGAGAGGCTAAGAAGGTACATTCCTGATGTTG
CGATAACTACAGACATAATGGTGGGATTTCCTGGGGAGACAGAGGAGGAGTTTGAAAAGAGCTATAAATTTGCTGAGGAG
ATATGCTTTGCTAAAATGCACGTATTCAAGTATTCTCGGAGGAAGGGCACAAGAGCGTATAATTTTCCGAATCAGGTCCC
AAATAAAGTAAAAGAAGAAAGGAGCAAAAAGCTTTTACAGCTCTCTAAAGTTTGTGAGAGAAAGTTTAAGGAAAGATTTT
TAAATAGGACAGTAGAAGTTCTGTTTGAGCAGAAGGTAAAAGATTTAGATGGATATGTGGAAGGGCTTACAGACAATTAC
CTTAGTGTGGCAGTTAAAGGAGAATTAGAAAATTTGAGAAACCGCATCTTGCCTGTTAGAGTAAAGGAAATAAAAAATGA
TTTATTGATAGGAGAAATTGAAGGAATTTATTAA

Upstream 100 bases:

>100_bases
TCTTTAAAATAAAAGTACAGTTTTGTTAATTTTAACAGCGATAGCTTTCTTAATATGTGGTATAATATAAAAAGACTTCA
AAGGGAGGAGTATTGGGAAA

Downstream 100 bases:

>100_bases
GATGTGTAGAATATAAAAAGAGGAGGTGAAAAAGGTGTCGGAATGCATTTTCTGCAAAATAGTAAACAAAGAAGTTCCTT
CTAATATAGTGTATGAGGAT

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MAKVAFYTLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQMIRKTRNKNPDAIVVAVGCYV
QVSPDEVFSMPEVDIVIGTKDKDKIVDLVKDFENEKKKTKLIENIMKQRDYEEFGITGYTERTRAYIKIEDGCNQYCTYC
IIPYARGPVRSRKPENIIKEVKKYAEHGYKEIVLTGIHIASYGRDLKNIGLLDVIKMVHEVEGIERIRISSIEPTFLTED
VVKELANLPKMCRHYHVSLQSGCDETLKRMGRKYTASEYKEVVERLRRYIPDVAITTDIMVGFPGETEEEFEKSYKFAEE
ICFAKMHVFKYSRRKGTRAYNFPNQVPNKVKEERSKKLLQLSKVCERKFKERFLNRTVEVLFEQKVKDLDGYVEGLTDNY
LSVAVKGELENLRNRILPVRVKEIKNDLLIGEIEGIY

Sequences:

>Translated_437_residues
MAKVAFYTLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQMIRKTRNKNPDAIVVAVGCYV
QVSPDEVFSMPEVDIVIGTKDKDKIVDLVKDFENEKKKTKLIENIMKQRDYEEFGITGYTERTRAYIKIEDGCNQYCTYC
IIPYARGPVRSRKPENIIKEVKKYAEHGYKEIVLTGIHIASYGRDLKNIGLLDVIKMVHEVEGIERIRISSIEPTFLTED
VVKELANLPKMCRHYHVSLQSGCDETLKRMGRKYTASEYKEVVERLRRYIPDVAITTDIMVGFPGETEEEFEKSYKFAEE
ICFAKMHVFKYSRRKGTRAYNFPNQVPNKVKEERSKKLLQLSKVCERKFKERFLNRTVEVLFEQKVKDLDGYVEGLTDNY
LSVAVKGELENLRNRILPVRVKEIKNDLLIGEIEGIY
>Mature_436_residues
AKVAFYTLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQMIRKTRNKNPDAIVVAVGCYVQ
VSPDEVFSMPEVDIVIGTKDKDKIVDLVKDFENEKKKTKLIENIMKQRDYEEFGITGYTERTRAYIKIEDGCNQYCTYCI
IPYARGPVRSRKPENIIKEVKKYAEHGYKEIVLTGIHIASYGRDLKNIGLLDVIKMVHEVEGIERIRISSIEPTFLTEDV
VKELANLPKMCRHYHVSLQSGCDETLKRMGRKYTASEYKEVVERLRRYIPDVAITTDIMVGFPGETEEEFEKSYKFAEEI
CFAKMHVFKYSRRKGTRAYNFPNQVPNKVKEERSKKLLQLSKVCERKFKERFLNRTVEVLFEQKVKDLDGYVEGLTDNYL
SVAVKGELENLRNRILPVRVKEIKNDLLIGEIEGIY

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=459, Percent_Identity=27.0152505446623, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI28872782, Length=459, Percent_Identity=27.0152505446623, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI93277076, Length=366, Percent_Identity=29.2349726775956, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=29.4784580498866, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1787057, Length=393, Percent_Identity=27.735368956743, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=439, Percent_Identity=27.5626423690205, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71996771, Length=275, Percent_Identity=26.9090909090909, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=27.437641723356, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI19922432, Length=369, Percent_Identity=29.5392953929539, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50772; Mature: 50641

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFYTLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQM
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCEEEEEECCCHHHHHHHHHHH
IRKTRNKNPDAIVVAVGCYVQVSPDEVFSMPEVDIVIGTKDKDKIVDLVKDFENEKKKTK
HHHHCCCCCCEEEEEEEEEEEECHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
LIENIMKQRDYEEFGITGYTERTRAYIKIEDGCNQYCTYCIIPYARGPVRSRKPENIIKE
HHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCCCCEEHEEEEECCCCCCCCCCHHHHHHH
VKKYAEHGYKEIVLTGIHIASYGRDLKNIGLLDVIKMVHEVEGIERIRISSIEPTFLTED
HHHHHHCCCCEEHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCHHHEEECCCCCHHHHHH
VVKELANLPKMCRHYHVSLQSGCDETLKRMGRKYTASEYKEVVERLRRYIPDVAITTDIM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHEE
VGFPGETEEEFEKSYKFAEEICFAKMHVFKYSRRKGTRAYNFPNQVPNKVKEERSKKLLQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LSKVCERKFKERFLNRTVEVLFEQKVKDLDGYVEGLTDNYLSVAVKGELENLRNRILPVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHH
VKEIKNDLLIGEIEGIY
HHHHHCCCEEEECCCCC
>Mature Secondary Structure 
AKVAFYTLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCEEEEEECCCHHHHHHHHHHH
IRKTRNKNPDAIVVAVGCYVQVSPDEVFSMPEVDIVIGTKDKDKIVDLVKDFENEKKKTK
HHHHCCCCCCEEEEEEEEEEEECHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
LIENIMKQRDYEEFGITGYTERTRAYIKIEDGCNQYCTYCIIPYARGPVRSRKPENIIKE
HHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCCCCEEHEEEEECCCCCCCCCCHHHHHHH
VKKYAEHGYKEIVLTGIHIASYGRDLKNIGLLDVIKMVHEVEGIERIRISSIEPTFLTED
HHHHHHCCCCEEHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCHHHEEECCCCCHHHHHH
VVKELANLPKMCRHYHVSLQSGCDETLKRMGRKYTASEYKEVVERLRRYIPDVAITTDIM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHEE
VGFPGETEEEFEKSYKFAEEICFAKMHVFKYSRRKGTRAYNFPNQVPNKVKEERSKKLLQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LSKVCERKFKERFLNRTVEVLFEQKVKDLDGYVEGLTDNYLSVAVKGELENLRNRILPVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHH
VKEIKNDLLIGEIEGIY
HHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]