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The map label for this gene is AcoA2 [H]

Identifier: 20807175

GI number: 20807175

Start: 692507

End: 693496

Strand: Direct

Name: AcoA2 [H]

Synonym: TTE0688

Alternate gene names: 20807175

Gene position: 692507-693496 (Clockwise)

Preceding gene: 20807174

Following gene: 20807176

Centisome position: 25.75

GC content: 45.35

Gene sequence:

>990_bases
ATGAACATTCCTAAAGAAACTCTTATGCGTATGTATCTTGAGATGGTTACAATTCGATTATATGAGGAAACCATGGCTGA
AGCCTATCAGGAAGGGAAATACCCAGTATTCAACATTGCCTCAGGCCCAGTCCCTGGTGAGATGCATTTAGCTGCAGGAC
AGGAGCCTGTAGCTGTAGGGGTATGCATGCACCTTAAAAAGGAGGATGCTGTAGTCGGTACTCACCGGCCTCACCACTTT
GCTATTGCAAAAGGAGTAGACCTCAAACGCATGACAGCAGAAATATTTGGTAAAGTTACCGGTTTAGGCCGCGGCAAAGG
TGGTCACATGCACCTCTTTGACCCTGATGTACACTTCAGCTGTAGCGGCATTGTTGGTGCTAGCATTCCACAAGCAGTAG
GAGCAGCTCTAGCTTTCAAAATGCGCAAAGAAAAGCGTGTAGCTGTGGCCTTTTTTGGTGACGGTGCTGCAAATCAGGGT
ACTTTTCATGAGGGACTTAACTTAGCTGCAATTTGGAAGCTACCGGTGGTGTTTGTATGTGAGGACAATAGTTGGGCAAT
TTCTGTACCAAAAGAAAAGTCTACAGCTATCAGTCAAAATGCTGACCGTGCAGTTGCGTATGGTATACCTGGAGTATACG
TGGGAGAGAACGATGTCTTAGCTGTTTACGAAGCTGCTGGCGAAGCAATAGAGAGAGCACGTCAGGGAGGAGGACCAACT
ATTATTGAAGTTAAAACAGATCGTTTCTTTGGACATTTCCAGGGTGATCCAGAAGTATATAGACCTAAGGACGAAGTTCA
GCGTCTAAAGCAAAATGATCCAATTAAGCGGTTCAGGAAATACCTTATTGAAAATGACATAGCCACAGAAGATGAACTTA
AACAATTAGACGATGAGGCACGAAAACGTGTTGAGGAGGCTTTCTTGTTTGCTCGGGAGTCACCCTATCCAGCGCCGGAA
GAAGCCCTACTTCACGTGTTTGTAGAATAG

Upstream 100 bases:

>100_bases
AACAAAAAATTAAAACATATTCACCTTCTTTGACATTATAAAGCAGGGTAATATATTGCAAAAAATTGAAAAAATTATAA
ATAGATAGGAGTGAAGAGGT

Downstream 100 bases:

>100_bases
TGAATTGCGGTCTAGTTGAATAATATTTAAAGGAGGAGAAAATTATGTCTGCGATAACTTCGCGAAAGCTTACTATGGCA
AAGGCAATTGCTGAAGCTAT

Product: thiamine pyrophosphate-dependent dehydrogenase, E1 component alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNIPKETLMRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALAFKMRKEKRVAVAFFGDGAANQG
TFHEGLNLAAIWKLPVVFVCEDNSWAISVPKEKSTAISQNADRAVAYGIPGVYVGENDVLAVYEAAGEAIERARQGGGPT
IIEVKTDRFFGHFQGDPEVYRPKDEVQRLKQNDPIKRFRKYLIENDIATEDELKQLDDEARKRVEEAFLFARESPYPAPE
EALLHVFVE

Sequences:

>Translated_329_residues
MNIPKETLMRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALAFKMRKEKRVAVAFFGDGAANQG
TFHEGLNLAAIWKLPVVFVCEDNSWAISVPKEKSTAISQNADRAVAYGIPGVYVGENDVLAVYEAAGEAIERARQGGGPT
IIEVKTDRFFGHFQGDPEVYRPKDEVQRLKQNDPIKRFRKYLIENDIATEDELKQLDDEARKRVEEAFLFARESPYPAPE
EALLHVFVE
>Mature_329_residues
MNIPKETLMRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALAFKMRKEKRVAVAFFGDGAANQG
TFHEGLNLAAIWKLPVVFVCEDNSWAISVPKEKSTAISQNADRAVAYGIPGVYVGENDVLAVYEAAGEAIERARQGGGPT
IIEVKTDRFFGHFQGDPEVYRPKDEVQRLKQNDPIKRFRKYLIENDIATEDELKQLDDEARKRVEEAFLFARESPYPAPE
EALLHVFVE

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=34.5794392523364, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI4505685, Length=327, Percent_Identity=33.9449541284404, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI291084742, Length=327, Percent_Identity=33.9449541284404, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI11386135, Length=331, Percent_Identity=32.3262839879154, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI258645172, Length=331, Percent_Identity=32.02416918429, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI291084757, Length=327, Percent_Identity=29.9694189602446, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32564172, Length=329, Percent_Identity=36.7781155015198, Blast_Score=214, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17536047, Length=329, Percent_Identity=36.7781155015198, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI86563357, Length=311, Percent_Identity=31.5112540192926, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI86563355, Length=311, Percent_Identity=31.5112540192926, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321026, Length=327, Percent_Identity=37.6146788990826, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24639748, Length=305, Percent_Identity=38.6885245901639, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24639744, Length=313, Percent_Identity=36.1022364217252, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28571106, Length=313, Percent_Identity=36.1022364217252, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24639740, Length=315, Percent_Identity=35.8730158730159, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24639746, Length=301, Percent_Identity=36.8770764119601, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21355903, Length=330, Percent_Identity=25.7575757575758, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36399; Mature: 36399

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPKETLMRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEE
VCMHLKKEDAVVGTHRPHHFAIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFS
EEEEECCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
CSGIVGASIPQAVGAALAFKMRKEKRVAVAFFGDGAANQGTFHEGLNLAAIWKLPVVFVC
ECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHCCCEEEEEECCEEEEE
EDNSWAISVPKEKSTAISQNADRAVAYGIPGVYVGENDVLAVYEAAGEAIERARQGGGPT
ECCCEEEECCCCCCHHHHCCCCCEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCE
IIEVKTDRFFGHFQGDPEVYRPKDEVQRLKQNDPIKRFRKYLIENDIATEDELKQLDDEA
EEEEECCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
RKRVEEAFLFARESPYPAPEEALLHVFVE
HHHHHHHHHHHCCCCCCCCHHHHEEEECC
>Mature Secondary Structure
MNIPKETLMRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEE
VCMHLKKEDAVVGTHRPHHFAIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFS
EEEEECCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
CSGIVGASIPQAVGAALAFKMRKEKRVAVAFFGDGAANQGTFHEGLNLAAIWKLPVVFVC
ECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHCCCEEEEEECCEEEEE
EDNSWAISVPKEKSTAISQNADRAVAYGIPGVYVGENDVLAVYEAAGEAIERARQGGGPT
ECCCEEEECCCCCCHHHHCCCCCEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCE
IIEVKTDRFFGHFQGDPEVYRPKDEVQRLKQNDPIKRFRKYLIENDIATEDELKQLDDEA
EEEEECCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
RKRVEEAFLFARESPYPAPEEALLHVFVE
HHHHHHHHHHHCCCCCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]