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The map label for this gene is GcvH2

Identifier: 20806816

GI number: 20806816

Start: 311393

End: 311773

Strand: Reverse

Name: GcvH2

Synonym: TTE0295

Alternate gene names: 20806816

Gene position: 311773-311393 (Counterclockwise)

Preceding gene: 20806817

Following gene: 20806815

Centisome position: 11.59

GC content: 40.68

Gene sequence:

>381_bases
ATGGAAGTTTTAGAAGGCCTTTATTATTCAAAAGACCATGAGTGGGTAAAAGTAGAGGGAGACAAGGTATACATAGGAAT
CACAGACTATGCTCAGCATTCACTGGGAAACATCGTTTACGTTGAACTTCCGGAGGTAGGATCTGAACTTTCTGCTGGAG
ATGTACTTGGAGTAGTAGAATCTGTAAAAGCTGCTTCAGACGTGTACACTCCAGTAGACATAAAAGTGTTGGAAGTAAAC
AATTCTCTGGTGGAAGACCCATCCCTTATAAACAATGACCCATATGGAAGCTGGATGGTGATAGCAGAGCTTAAAGACAA
ATCTCAGTTAGATAAACTAATGAAGGCCGATGAGTACAGAAAATTCTTAGAGGAGGAGTGA

Upstream 100 bases:

>100_bases
AAATCAAATTGAGGTAATAATAAGAAATAAGCCGCTTAAAGCGGTGATAGTAGATAAGAATTTCTACAAGAAAAATTACA
AAAAATAGGAGGGTTTGAAC

Downstream 100 bases:

>100_bases
TAGTTATGTTTCCGTATCTCCCCATCTCCTCCGAAGATGAAAAGGAAATGCTAAAAACCATAGGAAAAAACTCTATTGAA
GAATTATTTGAAGTCATCCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MEVLEGLYYSKDHEWVKVEGDKVYIGITDYAQHSLGNIVYVELPEVGSELSAGDVLGVVESVKAASDVYTPVDIKVLEVN
NSLVEDPSLINNDPYGSWMVIAELKDKSQLDKLMKADEYRKFLEEE

Sequences:

>Translated_126_residues
MEVLEGLYYSKDHEWVKVEGDKVYIGITDYAQHSLGNIVYVELPEVGSELSAGDVLGVVESVKAASDVYTPVDIKVLEVN
NSLVEDPSLINNDPYGSWMVIAELKDKSQLDKLMKADEYRKFLEEE
>Mature_126_residues
MEVLEGLYYSKDHEWVKVEGDKVYIGITDYAQHSLGNIVYVELPEVGSELSAGDVLGVVESVKAASDVYTPVDIKVLEVN
NSLVEDPSLINNDPYGSWMVIAELKDKSQLDKLMKADEYRKFLEEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=41.025641025641, Blast_Score=107, Evalue=3e-24,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=40.1709401709402, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=48.3606557377049, Blast_Score=121, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=45.0819672131148, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=44.2622950819672, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=124, Percent_Identity=45.1612903225806, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=44.4444444444444, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH2_THETN (Q8RCW0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621987.1
- ProteinModelPortal:   Q8RCW0
- SMR:   Q8RCW0
- GeneID:   996548
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0295
- NMPDR:   fig|273068.3.peg.45
- HOGENOM:   HBG693789
- OMA:   NDDPYDG
- ProtClustDB:   PRK01202
- BioCyc:   TTEN273068:TTE0295-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14158; Mature: 14158

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLEGLYYSKDHEWVKVEGDKVYIGITDYAQHSLGNIVYVELPEVGSELSAGDVLGVVE
CCCCCCCEECCCCCEEEEECCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
SVKAASDVYTPVDIKVLEVNNSLVEDPSLINNDPYGSWMVIAELKDKSQLDKLMKADEYR
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KFLEEE
HHHCCC
>Mature Secondary Structure
MEVLEGLYYSKDHEWVKVEGDKVYIGITDYAQHSLGNIVYVELPEVGSELSAGDVLGVVE
CCCCCCCEECCCCCEEEEECCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
SVKAASDVYTPVDIKVLEVNNSLVEDPSLINNDPYGSWMVIAELKDKSQLDKLMKADEYR
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KFLEEE
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336