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The map label for this gene is gcvT

Identifier: 20806817

GI number: 20806817

Start: 311786

End: 312910

Strand: Reverse

Name: gcvT

Synonym: TTE0296

Alternate gene names: 20806817

Gene position: 312910-311786 (Counterclockwise)

Preceding gene: 20806839

Following gene: 20806816

Centisome position: 11.63

GC content: 37.33

Gene sequence:

>1125_bases
ATGAAAGGGTGTGATAGTTTGGATAATCTCAAAAAAACCCCATTGTATGAAATTTATCCTAAGTACAATGCAAAAATAAT
AGACTTTGCCGGCTGGGCAATGCCAGTTCAATTCGAAAGCATAATATCAGAACACGAAGCTGTCAGAAATGCAGCAGGCC
TTTTCGATGTATCCCATATGGGAGAAATAATTGTAAAAGGAAAAGATGCGTTTCCTTTCCTCCAGAACCTCCTCACAAAT
GACCTTTCCAAGTTAAATGACAATCAGGTGCTTTACACTTTCATGTGCAATCACAACGGAGGAGTAATTGACGACCTTCT
AGTCTACAAGTATTCCAACAACTACTATCTTTTAGTAGTTAATGCAGCAAACATAGAAAAGGATTACAAATGGATGCTGA
ACAATGCAGGAATATACAAAGTGGAAATTGAAAATGTGTCAGATAAGATAGCAGAACTAGCAATTCAGGGTCCAAAAGCA
GAAGAAATCCTTCAAAAATTGACTGATGAGGACCTATCACAGATCAAGTTCTTCTACTTCAAAGATAAAGTAAAAATTGC
TGGTGTAGAATGCTTAGTTTCCCGCACAGGTTACACTGGTGAAGACGGATTTGAAATTTATATGCCAAATGAACATGCTG
TCACCCTTTGGGAGAAAATTTTAGAGGCGGGCAAAGATTACGGCCTCAAGCCGGCAGGTCTTGGCGCAAGAGATACACTT
CGCTTTGAAGCAGGTTTGCCTCTTTATGGAAATGAATTAGGAGAGGACATAACTCCTTTAGAAGCAGGTTTAGGATTTTT
TGTGAAGTTTGACAAAGGAAATTTTATTGGAAAAGACGCTCTTTTAAAACAGAAGGAACAAGGATTAAAGAGAAAACTGG
TAGGATTTGAAATGATAGGCAATGGAATTCCAAGACACGGGTATGAAGTACAGGCTGATAATCAAAAAATTGGCTATGTG
ACTACGGGTTATTTCTCTCCAACTCTTAAAAAGAACATAGGACTCGCTTTGATTGACTCAAAATACGCCCAAATAGGAAA
TCAAATTGAGGTAATAATAAGAAATAAGCCGCTTAAAGCGGTGATAGTAGATAAGAATTTCTACAAGAAAAATTACAAAA
AATAG

Upstream 100 bases:

>100_bases
ATGGAAGGCCATATGTAGAAGCTCTCAGGTAGAAAAACAGAGGAGTTGTGATGGCACTTAATCTTTTCGTGCTCACTCAC
AGCTCCTCTGTTTAAATTTT

Downstream 100 bases:

>100_bases
GAGGGTTTGAACATGGAAGTTTTAGAAGGCCTTTATTATTCAAAAGACCATGAGTGGGTAAAAGTAGAGGGAGACAAGGT
ATACATAGGAATCACAGACT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKGCDSLDNLKKTPLYEIYPKYNAKIIDFAGWAMPVQFESIISEHEAVRNAAGLFDVSHMGEIIVKGKDAFPFLQNLLTN
DLSKLNDNQVLYTFMCNHNGGVIDDLLVYKYSNNYYLLVVNAANIEKDYKWMLNNAGIYKVEIENVSDKIAELAIQGPKA
EEILQKLTDEDLSQIKFFYFKDKVKIAGVECLVSRTGYTGEDGFEIYMPNEHAVTLWEKILEAGKDYGLKPAGLGARDTL
RFEAGLPLYGNELGEDITPLEAGLGFFVKFDKGNFIGKDALLKQKEQGLKRKLVGFEMIGNGIPRHGYEVQADNQKIGYV
TTGYFSPTLKKNIGLALIDSKYAQIGNQIEVIIRNKPLKAVIVDKNFYKKNYKK

Sequences:

>Translated_374_residues
MKGCDSLDNLKKTPLYEIYPKYNAKIIDFAGWAMPVQFESIISEHEAVRNAAGLFDVSHMGEIIVKGKDAFPFLQNLLTN
DLSKLNDNQVLYTFMCNHNGGVIDDLLVYKYSNNYYLLVVNAANIEKDYKWMLNNAGIYKVEIENVSDKIAELAIQGPKA
EEILQKLTDEDLSQIKFFYFKDKVKIAGVECLVSRTGYTGEDGFEIYMPNEHAVTLWEKILEAGKDYGLKPAGLGARDTL
RFEAGLPLYGNELGEDITPLEAGLGFFVKFDKGNFIGKDALLKQKEQGLKRKLVGFEMIGNGIPRHGYEVQADNQKIGYV
TTGYFSPTLKKNIGLALIDSKYAQIGNQIEVIIRNKPLKAVIVDKNFYKKNYKK
>Mature_374_residues
MKGCDSLDNLKKTPLYEIYPKYNAKIIDFAGWAMPVQFESIISEHEAVRNAAGLFDVSHMGEIIVKGKDAFPFLQNLLTN
DLSKLNDNQVLYTFMCNHNGGVIDDLLVYKYSNNYYLLVVNAANIEKDYKWMLNNAGIYKVEIENVSDKIAELAIQGPKA
EEILQKLTDEDLSQIKFFYFKDKVKIAGVECLVSRTGYTGEDGFEIYMPNEHAVTLWEKILEAGKDYGLKPAGLGARDTL
RFEAGLPLYGNELGEDITPLEAGLGFFVKFDKGNFIGKDALLKQKEQGLKRKLVGFEMIGNGIPRHGYEVQADNQKIGYV
TTGYFSPTLKKNIGLALIDSKYAQIGNQIEVIIRNKPLKAVIVDKNFYKKNYKK

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=371, Percent_Identity=35.3099730458221, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI257796258, Length=356, Percent_Identity=35.1123595505618, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI257796254, Length=368, Percent_Identity=32.6086956521739, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI24797151, Length=333, Percent_Identity=33.3333333333333, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI257796256, Length=316, Percent_Identity=35.126582278481, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI197927446, Length=335, Percent_Identity=30.1492537313433, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI21361378, Length=335, Percent_Identity=30.1492537313433, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI194306651, Length=316, Percent_Identity=27.5316455696203, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789272, Length=374, Percent_Identity=40.9090909090909, Blast_Score=264, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17560118, Length=379, Percent_Identity=37.7308707124011, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI32563613, Length=316, Percent_Identity=29.4303797468354, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994045, Length=327, Percent_Identity=27.5229357798165, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994052, Length=327, Percent_Identity=27.5229357798165, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320222, Length=391, Percent_Identity=32.9923273657289, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=35.0132625994695, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI28571104, Length=283, Percent_Identity=30.7420494699647, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI20130091, Length=344, Percent_Identity=26.453488372093, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_THETN (Q8RCV9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621988.1
- ProteinModelPortal:   Q8RCV9
- SMR:   Q8RCV9
- GeneID:   997936
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0296
- NMPDR:   fig|273068.3.peg.46
- HOGENOM:   HBG299834
- OMA:   NDISKPP
- ProtClustDB:   PRK00389
- BioCyc:   TTEN273068:TTE0296-MONOMER
- BRENDA:   2.1.2.10
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 42167; Mature: 42167

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGCDSLDNLKKTPLYEIYPKYNAKIIDFAGWAMPVQFESIISEHEAVRNAAGLFDVSHM
CCCCCCHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHC
GEIIVKGKDAFPFLQNLLTNDLSKLNDNQVLYTFMCNHNGGVIDDLLVYKYSNNYYLLVV
CEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCCEEEEEE
NAANIEKDYKWMLNNAGIYKVEIENVSDKIAELAIQGPKAEEILQKLTDEDLSQIKFFYF
ECCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHEEEEEE
KDKVKIAGVECLVSRTGYTGEDGFEIYMPNEHAVTLWEKILEAGKDYGLKPAGLGARDTL
ECCEEEECEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
RFEAGLPLYGNELGEDITPLEAGLGFFVKFDKGNFIGKDALLKQKEQGLKRKLVGFEMIG
EEECCCCCCCHHHCCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NGIPRHGYEVQADNQKIGYVTTGYFSPTLKKNIGLALIDSKYAQIGNQIEVIIRNKPLKA
CCCCCCCEEEEECCCEEEEEEECCCCCHHHHCCCEEEECCHHHHCCCEEEEEEECCCEEE
VIVDKNFYKKNYKK
EEECCHHHHCCCCC
>Mature Secondary Structure
MKGCDSLDNLKKTPLYEIYPKYNAKIIDFAGWAMPVQFESIISEHEAVRNAAGLFDVSHM
CCCCCCHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHC
GEIIVKGKDAFPFLQNLLTNDLSKLNDNQVLYTFMCNHNGGVIDDLLVYKYSNNYYLLVV
CEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCCEEEEEE
NAANIEKDYKWMLNNAGIYKVEIENVSDKIAELAIQGPKAEEILQKLTDEDLSQIKFFYF
ECCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHEEEEEE
KDKVKIAGVECLVSRTGYTGEDGFEIYMPNEHAVTLWEKILEAGKDYGLKPAGLGARDTL
ECCEEEECEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
RFEAGLPLYGNELGEDITPLEAGLGFFVKFDKGNFIGKDALLKQKEQGLKRKLVGFEMIG
EEECCCCCCCHHHCCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NGIPRHGYEVQADNQKIGYVTTGYFSPTLKKNIGLALIDSKYAQIGNQIEVIIRNKPLKA
CCCCCCCEEEEECCCEEEEEEECCCCCHHHHCCCEEEECCHHHHCCCEEEEEEECCCEEE
VIVDKNFYKKNYKK
EEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336