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The map label for this gene is prfA [H]

Identifier: 20806672

GI number: 20806672

Start: 141648

End: 142718

Strand: Direct

Name: prfA [H]

Synonym: TTE0143

Alternate gene names: 20806672

Gene position: 141648-142718 (Clockwise)

Preceding gene: 20806671

Following gene: 20806673

Centisome position: 5.27

GC content: 41.36

Gene sequence:

>1071_bases
ATGATTGAAAAGTTGCAGGCAATAGAGGATAGGTATGTAGAATTAAGTCAAAAGATAAGCGACCCTAATGTCATTTCCAA
TGTAGAAGAATGGAGAAAATACGTAAAAGAACACGCAGCTATTGAAGACATCGTTTTAAAGTATAGGGAGTATAAAAAGG
TTTTGGAAGAAATAAAAGCGACAGAAGAGCTTTTGTCCTCTCAGGATGAGGAACTTAAAGAGATGGCGGAAGAAGAGCTC
TCTCAACTGGAAGAAAAAAAAGAAAAACTGATGGAAGAGATAAAGATTTTGCTGCTCCCAAAAGACCCTAATGATGAGAA
AAACGTCATAATGGAGATAAGAGCCGGAGCAGGAGGAGAGGAAGCTGCCCTTTTTGCCTATGACCTTTTCAGGATGTACA
CGATGTATGCGGAGAAAAAAGGCTGGAAAGTTGAGATAATGAGCGCCAACAAGACAGATATTGGAGGTTTTAAGGAAGTA
ATTTTTAGCATTTCTGGAAAAGGAGCCTATAGCAGGCTTAAATATGAAAGCGGCGTTCACAGGGTTCAGAGGATTCCTAC
TACTGAGGCAGGAGGAAGAATTCATACTTCTACTGCCACAGTAGCAGTTCTGCCAGAAGCCGAAGAGGTGGATGTTGAGA
TAAATCCAAATGATATAAAAATAGATGTTTTCAGGTCAAGTGGGCATGGCGGGCAGAGCGTGAATACTACGGATTCAGCA
GTAAGAGTTACCCACATTCCTACAGGGATTGTGGTAACCTGTCAAGATGAAAGGTCTCAGATTCAAAATAGGGAGAGAGC
TTTAAAGATTTTAAGGGCAAAGCTTTATGAGATGGCTCTAAAGGAACAGCAGAGGGAAATAGCCGAAGCTAGAAAGTCTC
AGGTTGGCACAGGGGAGAGAAGTGAGAGGATAAGGACTTACAATTTTCCTCAGGGAAGAGTTACAGACCACAGGATAGGA
CTTACTCTTTACAAGCTCCAAGAGGTTTTGGATGGGGAGCTGGACGAAATCATAGATGCGTTGATTTTAAATGACCAGGC
GGAAAAACTTAAGAATATGACTCTTAACTGA

Upstream 100 bases:

>100_bases
AAAAGATTTAGCTCATATAGATAGAGTTATAATCGGTAGATATAACTCGTAGTTTTCACTTTTTTTATCATGTGATATAA
TGTTTTTCTGAGGTGAGATA

Downstream 100 bases:

>100_bases
TTTTCTCTGTTACCACTTGACTAAATTGCTTTTATAAAGTATAATTTATTAAAGATGCTTCATCACAATAAATCAAAAAA
TTAGCCCCCCAGCTGTCCTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIEKLQAIEDRYVELSQKISDPNVISNVEEWRKYVKEHAAIEDIVLKYREYKKVLEEIKATEELLSSQDEELKEMAEEEL
SQLEEKKEKLMEEIKILLLPKDPNDEKNVIMEIRAGAGGEEAALFAYDLFRMYTMYAEKKGWKVEIMSANKTDIGGFKEV
IFSISGKGAYSRLKYESGVHRVQRIPTTEAGGRIHTSTATVAVLPEAEEVDVEINPNDIKIDVFRSSGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALKEQQREIAEARKSQVGTGERSERIRTYNFPQGRVTDHRIG
LTLYKLQEVLDGELDEIIDALILNDQAEKLKNMTLN

Sequences:

>Translated_356_residues
MIEKLQAIEDRYVELSQKISDPNVISNVEEWRKYVKEHAAIEDIVLKYREYKKVLEEIKATEELLSSQDEELKEMAEEEL
SQLEEKKEKLMEEIKILLLPKDPNDEKNVIMEIRAGAGGEEAALFAYDLFRMYTMYAEKKGWKVEIMSANKTDIGGFKEV
IFSISGKGAYSRLKYESGVHRVQRIPTTEAGGRIHTSTATVAVLPEAEEVDVEINPNDIKIDVFRSSGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALKEQQREIAEARKSQVGTGERSERIRTYNFPQGRVTDHRIG
LTLYKLQEVLDGELDEIIDALILNDQAEKLKNMTLN
>Mature_356_residues
MIEKLQAIEDRYVELSQKISDPNVISNVEEWRKYVKEHAAIEDIVLKYREYKKVLEEIKATEELLSSQDEELKEMAEEEL
SQLEEKKEKLMEEIKILLLPKDPNDEKNVIMEIRAGAGGEEAALFAYDLFRMYTMYAEKKGWKVEIMSANKTDIGGFKEV
IFSISGKGAYSRLKYESGVHRVQRIPTTEAGGRIHTSTATVAVLPEAEEVDVEINPNDIKIDVFRSSGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALKEQQREIAEARKSQVGTGERSERIRTYNFPQGRVTDHRIG
LTLYKLQEVLDGELDEIIDALILNDQAEKLKNMTLN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=287, Percent_Identity=49.1289198606272, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI34577120, Length=327, Percent_Identity=44.0366972477064, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI166795305, Length=192, Percent_Identity=46.875, Blast_Score=198, Evalue=6e-51,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=50.9859154929577, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=39.3491124260355, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=303, Percent_Identity=36.963696369637, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321295, Length=328, Percent_Identity=46.3414634146341, Blast_Score=274, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19921226, Length=317, Percent_Identity=43.217665615142, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40633; Mature: 40633

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKLQAIEDRYVELSQKISDPNVISNVEEWRKYVKEHAAIEDIVLKYREYKKVLEEIKA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEELLSSQDEELKEMAEEELSQLEEKKEKLMEEIKILLLPKDPNDEKNVIMEIRAGAGGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAYDLFRMYTMYAEKKGWKVEIMSANKTDIGGFKEVIFSISGKGAYSRLKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
RVQRIPTTEAGGRIHTSTATVAVLPEAEEVDVEINPNDIKIDVFRSSGHGGQSVNTTDSA
HHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALKEQQREIAEARKSQVGTGER
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SERIRTYNFPQGRVTDHRIGLTLYKLQEVLDGELDEIIDALILNDQAEKLKNMTLN
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MIEKLQAIEDRYVELSQKISDPNVISNVEEWRKYVKEHAAIEDIVLKYREYKKVLEEIKA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEELLSSQDEELKEMAEEELSQLEEKKEKLMEEIKILLLPKDPNDEKNVIMEIRAGAGGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAYDLFRMYTMYAEKKGWKVEIMSANKTDIGGFKEVIFSISGKGAYSRLKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
RVQRIPTTEAGGRIHTSTATVAVLPEAEEVDVEINPNDIKIDVFRSSGHGGQSVNTTDSA
HHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALKEQQREIAEARKSQVGTGER
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SERIRTYNFPQGRVTDHRIGLTLYKLQEVLDGELDEIIDALILNDQAEKLKNMTLN
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA