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The map label for this gene is HemK [H]

Identifier: 20806671

GI number: 20806671

Start: 140742

End: 141599

Strand: Direct

Name: HemK [H]

Synonym: TTE0142

Alternate gene names: 20806671

Gene position: 140742-141599 (Clockwise)

Preceding gene: 20806670

Following gene: 20806672

Centisome position: 5.23

GC content: 35.9

Gene sequence:

>858_bases
ATGAAGGGGGGCGGGAGTTTGAAAGTATATGAGGCAATAGCTCTGGGGGCTAAAGAGCTTGAAAGCGTCTGTCCAAATCC
TCGACTTGAATCTGAGCTTTTGCTTTCTCATTCTCTTAGGATTGACAGAGTTAAACTTGCAATTAAAAGAGATGAGGTTT
TGAAAGAAGAGGAATTGGAGAGGTTTTTAAAGTATATAGAAAAAAGAAAATCCCGCATACCCTATCAGTATATTGTAAAA
AAACAGCATTTCATGGGATTTGAGTTTTATGTGGATGAGAGGGTGCTAATTCCAAGACCTGAAACAGAAATTCTTGTGGA
AGAAGCGTTAAAGAGGATGAAAAGTGGAGATTTGATACTTGATATAGGTACAGGAAGTGGCGCCATAGCTATAAGCATAG
CCAAACTTTTTCCTGATTGTAAGGTGTACGCTGTAGATGTGAGTGAAGAAGCTTTAGAGGTTGCCAAGTATAATGCAGAA
AAACTTGGCGTGGCTGAAAAAATTATATTTATAAAGAGCGATATTTTTTCCAATATACCTCAAGATGTAAAATTTGATTT
AATTGTCTCCAACCCCCCTTATATAAAAAAAGCAGAACTTGAGAATTTACAAGAAGAAGTTAAAAAAGAGCCTATTTTAG
CACTTGACGGAGGAGAAGATGGGCTTTTTTTCTACAAAAGAATCATACCAGATTGTAAATTTTATTTGAAAAAGGGAGGA
AGGGGTTTGTTTGAAATAGGCTACGGCCAAAGAGAAGAGGTGGAAAAAATCTTTTTGGAGAATGGTTTTGATGAAATTGA
AGTGATAAAAGATTTAGCTCATATAGATAGAGTTATAATCGGTAGATATAACTCGTAG

Upstream 100 bases:

>100_bases
TTTGTGTATCCGGGATTGCTTTTGCAGAAGATCACCACTAAAGAGCCAGATGATAAGCAACTGGAGGTAGCAATTGCCTC
TTTAAAAAGCGTGCTTCAGG

Downstream 100 bases:

>100_bases
TTTTCACTTTTTTTATCATGTGATATAATGTTTTTCTGAGGTGAGATAATGATTGAAAAGTTGCAGGCAATAGAGGATAG
GTATGTAGAATTAAGTCAAA

Product: rRNA or tRNA methylase

Products: NA

Alternate protein names: M.BusHemKP [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKGGGSLKVYEAIALGAKELESVCPNPRLESELLLSHSLRIDRVKLAIKRDEVLKEEELERFLKYIEKRKSRIPYQYIVK
KQHFMGFEFYVDERVLIPRPETEILVEEALKRMKSGDLILDIGTGSGAIAISIAKLFPDCKVYAVDVSEEALEVAKYNAE
KLGVAEKIIFIKSDIFSNIPQDVKFDLIVSNPPYIKKAELENLQEEVKKEPILALDGGEDGLFFYKRIIPDCKFYLKKGG
RGLFEIGYGQREEVEKIFLENGFDEIEVIKDLAHIDRVIIGRYNS

Sequences:

>Translated_285_residues
MKGGGSLKVYEAIALGAKELESVCPNPRLESELLLSHSLRIDRVKLAIKRDEVLKEEELERFLKYIEKRKSRIPYQYIVK
KQHFMGFEFYVDERVLIPRPETEILVEEALKRMKSGDLILDIGTGSGAIAISIAKLFPDCKVYAVDVSEEALEVAKYNAE
KLGVAEKIIFIKSDIFSNIPQDVKFDLIVSNPPYIKKAELENLQEEVKKEPILALDGGEDGLFFYKRIIPDCKFYLKKGG
RGLFEIGYGQREEVEKIFLENGFDEIEVIKDLAHIDRVIIGRYNS
>Mature_285_residues
MKGGGSLKVYEAIALGAKELESVCPNPRLESELLLSHSLRIDRVKLAIKRDEVLKEEELERFLKYIEKRKSRIPYQYIVK
KQHFMGFEFYVDERVLIPRPETEILVEEALKRMKSGDLILDIGTGSGAIAISIAKLFPDCKVYAVDVSEEALEVAKYNAE
KLGVAEKIIFIKSDIFSNIPQDVKFDLIVSNPPYIKKAELENLQEEVKKEPILALDGGEDGLFFYKRIIPDCKFYLKKGG
RGLFEIGYGQREEVEKIFLENGFDEIEVIKDLAHIDRVIIGRYNS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=240, Percent_Identity=32.9166666666667, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1787463, Length=259, Percent_Identity=34.3629343629344, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI87082085, Length=228, Percent_Identity=39.4736842105263, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324266, Length=193, Percent_Identity=30.5699481865285, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582226, Length=235, Percent_Identity=32.7659574468085, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32580; Mature: 32580

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGGGSLKVYEAIALGAKELESVCPNPRLESELLLSHSLRIDRVKLAIKRDEVLKEEELE
CCCCCCEEHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCEEHHHEEEHHHHHHHHHHHH
RFLKYIEKRKSRIPYQYIVKKQHFMGFEFYVDERVLIPRPETEILVEEALKRMKSGDLIL
HHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEE
DIGTGSGAIAISIAKLFPDCKVYAVDVSEEALEVAKYNAEKLGVAEKIIFIKSDIFSNIP
EECCCCCEEEEEHHHHCCCCEEEEEECCHHHHHHHHHCHHHHCHHHHHEEEEHHHHHCCC
QDVKFDLIVSNPPYIKKAELENLQEEVKKEPILALDGGEDGLFFYKRIIPDCKFYLKKGG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
RGLFEIGYGQREEVEKIFLENGFDEIEVIKDLAHIDRVIIGRYNS
CEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGGGSLKVYEAIALGAKELESVCPNPRLESELLLSHSLRIDRVKLAIKRDEVLKEEELE
CCCCCCEEHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCEEHHHEEEHHHHHHHHHHHH
RFLKYIEKRKSRIPYQYIVKKQHFMGFEFYVDERVLIPRPETEILVEEALKRMKSGDLIL
HHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEE
DIGTGSGAIAISIAKLFPDCKVYAVDVSEEALEVAKYNAEKLGVAEKIIFIKSDIFSNIP
EECCCCCEEEEEHHHHCCCCEEEEEECCHHHHHHHHHCHHHHCHHHHHEEEEHHHHHCCC
QDVKFDLIVSNPPYIKKAELENLQEEVKKEPILALDGGEDGLFFYKRIIPDCKFYLKKGG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
RGLFEIGYGQREEVEKIFLENGFDEIEVIKDLAHIDRVIIGRYNS
CEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]