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The map label for this gene is PcnB [H]

Identifier: 20806638

GI number: 20806638

Start: 109853

End: 111253

Strand: Direct

Name: PcnB [H]

Synonym: TTE0109

Alternate gene names: 20806638

Gene position: 109853-111253 (Clockwise)

Preceding gene: 20806637

Following gene: 20806639

Centisome position: 4.08

GC content: 37.76

Gene sequence:

>1401_bases
GTGGCTACAATACCCAGGCTCATAAGGAAATTAGGAAGAGAAGCCTATAATGTTTACATTGTCGGCGGCTACATAAGGGA
CAGGATACTGAACAAAGAAGCAAAGGACTACGATTTTGTAGTTAAAGGCAATGCAGAAGAAATCGCAAAATTGGCAGCTG
AGAAGATGGGAGGCAGTTTTGTGCCTTTTATGGCAGAAAAAGGAACATATAGAATAGTGGTTGGAGATGAAATACTGGAT
TTCACTAATTTAAGAGGGAAAGACATATACGAAGATCTGGCCCACAGGGACTTTACAATAAATGCCATGGCTATTAGACT
CACTGACCACTTTGAATTTGAATACATAATAGACCCTTTTGGAGGATTAAAGGATATAAAAAACAGAAAAATAAGACATG
TGGCAGACTATACCTTTCGGGAAGACCCTTTGAGGGCTTTGAGGGCTGTGAGATTTGCTGCCACTTATAAATTTGATATA
GATGAGGCGACAAAAGCCAAAATAAAGGAAGAAGCCCATTTATTAAAAAATGTTGCCCCTGAGAGGATAATGAATGAAAT
TTTTATGATAATAAGAGAAAAAGATTCGCATAAGTATTTGAGGTTGATGGAAGAGCTAAGTTTGATGGAAAATATTTTTG
AAGAGGTAGCTAAACTCAAAGAGGTGGGTAAGTGCTACTATCAGCTTATAAACGCGTGGATACATTCTATAAGGGCAGTG
GAGGAGTATGAAAACATCATAAGCGCTATGAGATTTCCCAAGGATGTAGAAGACCTTGTGAGTGAATACCTAGAAAAACC
CATGTCTCCGGGAAATAAAGTTAAAGATATAATAAAACTGGGGATTTTGTTTCACGACATAGGCAAAGCTGATTCCATAT
ACATCGACGTAGAAAACCGCCTCCACTTTTACAATCACGAGATAAAAGGTGCTGAGATAATAAAGAAAATGGCCTCCCGC
ATGAGAATTCCCAAAAAAGAGACAAACCTCCTGAAAAAACTTGTGCTTTACCATAAAAAGCCCCTCGATTACTACATTGA
AGGGGTAAATAACAAGACACTTTTCAGGTTTTTCTATCATCTAAAAGATAACGCTATAGGTATTCTGCTAGCCTCTTTGG
CAGATTACACTGCCAGCCGGTTAAGCTTTGGGAAAGAAGAGGACATACCCCGTTATGAGAATTTTATAACAAAACTTTTA
AAGCGATATGTAGAGTTTAAAGAGACGGAAAAACCGCTGCTTTCTCCTCTAGACATAATATTAAATTTTGATGTGAAAGA
TGGCAAAAAGCTCGGGCAGATACTGTATGAGATAAGGAAAAACAGGTTTTACGGCGAAATAAAGACAAAAGAAGATGCCG
TTGAGTTTATAAAAGAGAGAATGAGAGTGGAGAAAATATGA

Upstream 100 bases:

>100_bases
TTTTTTACAGCCGCATATTTTTTTAGGCCTTGTATACTTTTTCCCTTTTGTTATACTTATTATTGGTAGGCAATTTACCT
TTTTGAAAGGAAGGATCTTC

Downstream 100 bases:

>100_bases
GGAGTTGAATAATAAAAAAGGGAGTTGACAATACTCATTATAAAGGGTATATTTAAGGATAAATAGAATATTGGAAAAGG
CGATGAAGGAAAGAGTAGGA

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MATIPRLIRKLGREAYNVYIVGGYIRDRILNKEAKDYDFVVKGNAEEIAKLAAEKMGGSFVPFMAEKGTYRIVVGDEILD
FTNLRGKDIYEDLAHRDFTINAMAIRLTDHFEFEYIIDPFGGLKDIKNRKIRHVADYTFREDPLRALRAVRFAATYKFDI
DEATKAKIKEEAHLLKNVAPERIMNEIFMIIREKDSHKYLRLMEELSLMENIFEEVAKLKEVGKCYYQLINAWIHSIRAV
EEYENIISAMRFPKDVEDLVSEYLEKPMSPGNKVKDIIKLGILFHDIGKADSIYIDVENRLHFYNHEIKGAEIIKKMASR
MRIPKKETNLLKKLVLYHKKPLDYYIEGVNNKTLFRFFYHLKDNAIGILLASLADYTASRLSFGKEEDIPRYENFITKLL
KRYVEFKETEKPLLSPLDIILNFDVKDGKKLGQILYEIRKNRFYGEIKTKEDAVEFIKERMRVEKI

Sequences:

>Translated_466_residues
MATIPRLIRKLGREAYNVYIVGGYIRDRILNKEAKDYDFVVKGNAEEIAKLAAEKMGGSFVPFMAEKGTYRIVVGDEILD
FTNLRGKDIYEDLAHRDFTINAMAIRLTDHFEFEYIIDPFGGLKDIKNRKIRHVADYTFREDPLRALRAVRFAATYKFDI
DEATKAKIKEEAHLLKNVAPERIMNEIFMIIREKDSHKYLRLMEELSLMENIFEEVAKLKEVGKCYYQLINAWIHSIRAV
EEYENIISAMRFPKDVEDLVSEYLEKPMSPGNKVKDIIKLGILFHDIGKADSIYIDVENRLHFYNHEIKGAEIIKKMASR
MRIPKKETNLLKKLVLYHKKPLDYYIEGVNNKTLFRFFYHLKDNAIGILLASLADYTASRLSFGKEEDIPRYENFITKLL
KRYVEFKETEKPLLSPLDIILNFDVKDGKKLGQILYEIRKNRFYGEIKTKEDAVEFIKERMRVEKI
>Mature_465_residues
ATIPRLIRKLGREAYNVYIVGGYIRDRILNKEAKDYDFVVKGNAEEIAKLAAEKMGGSFVPFMAEKGTYRIVVGDEILDF
TNLRGKDIYEDLAHRDFTINAMAIRLTDHFEFEYIIDPFGGLKDIKNRKIRHVADYTFREDPLRALRAVRFAATYKFDID
EATKAKIKEEAHLLKNVAPERIMNEIFMIIREKDSHKYLRLMEELSLMENIFEEVAKLKEVGKCYYQLINAWIHSIRAVE
EYENIISAMRFPKDVEDLVSEYLEKPMSPGNKVKDIIKLGILFHDIGKADSIYIDVENRLHFYNHEIKGAEIIKKMASRM
RIPKKETNLLKKLVLYHKKPLDYYIEGVNNKTLFRFFYHLKDNAIGILLASLADYTASRLSFGKEEDIPRYENFITKLLK
RYVEFKETEKPLLSPLDIILNFDVKDGKKLGQILYEIRKNRFYGEIKTKEDAVEFIKERMRVEKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=358, Percent_Identity=27.0949720670391, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081691, Length=236, Percent_Identity=29.2372881355932, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54734; Mature: 54603

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIPRLIRKLGREAYNVYIVGGYIRDRILNKEAKDYDFVVKGNAEEIAKLAAEKMGGSF
CCCHHHHHHHHCHHHEEEEEEEHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCC
VPFMAEKGTYRIVVGDEILDFTNLRGKDIYEDLAHRDFTINAMAIRLTDHFEFEYIIDPF
CCCEECCCCEEEEECCHHHHHHCCCCCHHHHHHHCCCEEEEEEEEEEECCCCEEEEECCC
GGLKDIKNRKIRHVADYTFREDPLRALRAVRFAATYKFDIDEATKAKIKEEAHLLKNVAP
CCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCH
ERIMNEIFMIIREKDSHKYLRLMEELSLMENIFEEVAKLKEVGKCYYQLINAWIHSIRAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYENIISAMRFPKDVEDLVSEYLEKPMSPGNKVKDIIKLGILFHDIGKADSIYIDVENR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
LHFYNHEIKGAEIIKKMASRMRIPKKETNLLKKLVLYHKKPLDYYIEGVNNKTLFRFFYH
EEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
LKDNAIGILLASLADYTASRLSFGKEEDIPRYENFITKLLKRYVEFKETEKPLLSPLDII
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHH
LNFDVKDGKKLGQILYEIRKNRFYGEIKTKEDAVEFIKERMRVEKI
EECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIPRLIRKLGREAYNVYIVGGYIRDRILNKEAKDYDFVVKGNAEEIAKLAAEKMGGSF
CCHHHHHHHHCHHHEEEEEEEHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCC
VPFMAEKGTYRIVVGDEILDFTNLRGKDIYEDLAHRDFTINAMAIRLTDHFEFEYIIDPF
CCCEECCCCEEEEECCHHHHHHCCCCCHHHHHHHCCCEEEEEEEEEEECCCCEEEEECCC
GGLKDIKNRKIRHVADYTFREDPLRALRAVRFAATYKFDIDEATKAKIKEEAHLLKNVAP
CCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCH
ERIMNEIFMIIREKDSHKYLRLMEELSLMENIFEEVAKLKEVGKCYYQLINAWIHSIRAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYENIISAMRFPKDVEDLVSEYLEKPMSPGNKVKDIIKLGILFHDIGKADSIYIDVENR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
LHFYNHEIKGAEIIKKMASRMRIPKKETNLLKKLVLYHKKPLDYYIEGVNNKTLFRFFYH
EEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
LKDNAIGILLASLADYTASRLSFGKEEDIPRYENFITKLLKRYVEFKETEKPLLSPLDII
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHH
LNFDVKDGKKLGQILYEIRKNRFYGEIKTKEDAVEFIKERMRVEKI
EECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]