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The map label for this gene is MetG

Identifier: 20806639

GI number: 20806639

Start: 111613

End: 113529

Strand: Direct

Name: MetG

Synonym: TTE0110

Alternate gene names: 20806639

Gene position: 111613-113529 (Clockwise)

Preceding gene: 20806638

Following gene: 20806640

Centisome position: 4.15

GC content: 39.18

Gene sequence:

>1917_bases
ATGAAAAAGACTTTTTACATCACCACTCCTATATATTACCCCAGTGACAAGCTACATATTGGCCATTCTTACACTACTGT
GGCTGCTGACGCAATGGCAAGGTTCAAAAGATTGACAGGGTATGACGTGATGTTTTTGACAGGTACAGACGAGCACGGGC
AGAAAATTCAGAGGATAGCCAGAGAAAAAGGCATGTCTCCAAAAGAATATGTGGACGGCATTGTGGAATGGATAAAAGAT
TTATGGAAAACAATGGATATAAGTTATGACCATTTTATTAGGACAACTGATGCCTATCACGAAGAAATAGTGCAAAAGAT
ATTCATGAAGTTGTACGAGCAGGGGGATATATATAAGGGAGAATACGAGGGATGGTATTGCACTCCTTGCGAGTCTTTCT
GGACAGAAAGCCAGCTTGTGGATGGAAAATGTCCTGACTGCGGAAGACCCGTTGAAAGGGTTACAGAGGAAGGGTATTTC
TTCAGACTTTCAGCTTATGGAGATAAGCTTTTGAAGTACTATGAGGAGCATCCAGATTTTATACAGCCAGAGTCCAGGCG
AAATGAAATGATAAACTTTATAAAGGCTGGATTGGAGGACTTATTTGTCTCAAGGAGCACTTTTGACTGGGGAATAAAAG
TGCCTTTTGACCCTAAGCACGTCATATACGTTTGGATAGATGCTCTTTCTAACTATATCACTGCATTAGGATATATGACA
GAAAATGATGAGAAATTTAAAAAGTATTGGCCTGCAGATGTGCACCTCGTTGGTAAGGAAATTGTGAGGTTCCACACGAT
AATATGGCCTGCTATGCTCATGGCTTTAGGACTTCCTCTTCCTAAAAAGGTGTTTGGGCATGGATGGTTGATTTTAGAAG
GAGGTAAAATGTCAAAATCCAAAGGGAATGTAGTGGACCCCAAGGAACTAGTGGACAGGTACGGAGTAGATGCTATAAGG
TATTTCCTCTTAAGAGAAGTTCCCTTTGGTGCTGACGGCGTATTTTCAAACGAAGCTTTAATAAACAGGATAAATTCCGA
TTTGGCAAATGATTTGGGCAATCTTTTAAGTAGAACTGTCACAATGATTGAGAAATACTTTGATGGAGTGTTGCCTAAAC
CTTCTTCTCAAGAAGAAATAGATGAAGACCTCATAAATGTAGCGCAAAATTTACCACAGAAAGTAGAAGAATACATGGAC
AAGTTGCAATTCTCCAACGCCTTAATAGAGATATGGAAATTAGTGTCAAGAGCTAATAAGTACATTGATGAGACTATGCC
GTGGGTGCTAGCAAAAGATGAATCTAAAAGAGGCAGACTGGGTACAGTTTTATACAATCTGGCAGAATCTTTAAGGTTCA
TAGGAATTTTGATATCTCCTTTTATGCCCAATACTCCTAAAAAGATGTTTGAACAATTAGGGATTACGGAAGATTTGACT
ACATGGGAAAGCTTAAAATTTGGGCTTTTGAAGGAAGGCACAAGGGTGAAGAGAGGAGAAATCCTGTTCCCCAGAATAGA
TGTTGAGAAAGAACTGGCATCTCTTGAAAAGAAAACTGAAGAAAAAACAAAAGAAACAAAAGAAGAGAAGATAGATTATA
TCACGATAGAAGATTTTTCAAAAGTTCAACTGAGAGTTGCTGAAATTTTAGAAGCTGAGAAAGTAGAAGGTTCAGACAAG
CTCATTAAAATGAAGCTTAAAGTCGGGGAAGAGATAAGGCAGATTGTAGGAGGAATTGGCAAATATTACAGTCCAGAAGA
GCTAATAGGCAAGAAGATAATAATTGTATATAATTTACAGCCGAGAAAGCTCATGGGGATTGAATCCCAGGGGATGCTAT
TGGCGGCCACTAATGAAGGGAAAATGGCGCTGTTGACGGTAGACAAAGACATAGAAAGCGGTTCTAAAATAAGCTGA

Upstream 100 bases:

>100_bases
GAGGTTCATGCGAAGAAGGGTGGTACCGCGAAAATCTCGCCCCTTGCCGGAGGCGAGTTTTTTATTTAATTACTATAAAA
AACTTGAGGAGGAGGTCAAA

Downstream 100 bases:

>100_bases
GGTGATAAAATTGCTTATAGACTCTCACGCTCATCTTGAGGATGAGAAATACAATGAAGACAGGGAAAAAGTGATTGAGG
AGTGCAAAAAGGACCTCACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVMFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKTMDISYDHFIRTTDAYHEEIVQKIFMKLYEQGDIYKGEYEGWYCTPCESFWTESQLVDGKCPDCGRPVERVTEEGYF
FRLSAYGDKLLKYYEEHPDFIQPESRRNEMINFIKAGLEDLFVSRSTFDWGIKVPFDPKHVIYVWIDALSNYITALGYMT
ENDEKFKKYWPADVHLVGKEIVRFHTIIWPAMLMALGLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVDRYGVDAIR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFDGVLPKPSSQEEIDEDLINVAQNLPQKVEEYMD
KLQFSNALIEIWKLVSRANKYIDETMPWVLAKDESKRGRLGTVLYNLAESLRFIGILISPFMPNTPKKMFEQLGITEDLT
TWESLKFGLLKEGTRVKRGEILFPRIDVEKELASLEKKTEEKTKETKEEKIDYITIEDFSKVQLRVAEILEAEKVEGSDK
LIKMKLKVGEEIRQIVGGIGKYYSPEELIGKKIIIVYNLQPRKLMGIESQGMLLAATNEGKMALLTVDKDIESGSKIS

Sequences:

>Translated_638_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVMFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKTMDISYDHFIRTTDAYHEEIVQKIFMKLYEQGDIYKGEYEGWYCTPCESFWTESQLVDGKCPDCGRPVERVTEEGYF
FRLSAYGDKLLKYYEEHPDFIQPESRRNEMINFIKAGLEDLFVSRSTFDWGIKVPFDPKHVIYVWIDALSNYITALGYMT
ENDEKFKKYWPADVHLVGKEIVRFHTIIWPAMLMALGLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVDRYGVDAIR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFDGVLPKPSSQEEIDEDLINVAQNLPQKVEEYMD
KLQFSNALIEIWKLVSRANKYIDETMPWVLAKDESKRGRLGTVLYNLAESLRFIGILISPFMPNTPKKMFEQLGITEDLT
TWESLKFGLLKEGTRVKRGEILFPRIDVEKELASLEKKTEEKTKETKEEKIDYITIEDFSKVQLRVAEILEAEKVEGSDK
LIKMKLKVGEEIRQIVGGIGKYYSPEELIGKKIIIVYNLQPRKLMGIESQGMLLAATNEGKMALLTVDKDIESGSKIS
>Mature_638_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVMFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKTMDISYDHFIRTTDAYHEEIVQKIFMKLYEQGDIYKGEYEGWYCTPCESFWTESQLVDGKCPDCGRPVERVTEEGYF
FRLSAYGDKLLKYYEEHPDFIQPESRRNEMINFIKAGLEDLFVSRSTFDWGIKVPFDPKHVIYVWIDALSNYITALGYMT
ENDEKFKKYWPADVHLVGKEIVRFHTIIWPAMLMALGLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVDRYGVDAIR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFDGVLPKPSSQEEIDEDLINVAQNLPQKVEEYMD
KLQFSNALIEIWKLVSRANKYIDETMPWVLAKDESKRGRLGTVLYNLAESLRFIGILISPFMPNTPKKMFEQLGITEDLT
TWESLKFGLLKEGTRVKRGEILFPRIDVEKELASLEKKTEEKTKETKEEKIDYITIEDFSKVQLRVAEILEAEKVEGSDK
LIKMKLKVGEEIRQIVGGIGKYYSPEELIGKKIIIVYNLQPRKLMGIESQGMLLAATNEGKMALLTVDKDIESGSKIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=542, Percent_Identity=35.9778597785978, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI14043022, Length=518, Percent_Identity=28.1853281853282, Blast_Score=174, Evalue=3e-43,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=29.4032023289665, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1786861, Length=173, Percent_Identity=27.7456647398844, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=34.8623853211009, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=34.8927875243665, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71992781, Length=404, Percent_Identity=33.6633663366337, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17540794, Length=589, Percent_Identity=26.4855687606112, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71997510, Length=168, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71997517, Length=168, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=32.3364485981308, Blast_Score=280, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6321704, Length=557, Percent_Identity=26.5709156193896, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323414, Length=202, Percent_Identity=27.7227722772277, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21358483, Length=552, Percent_Identity=32.4275362318841, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24655311, Length=610, Percent_Identity=26.0655737704918, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355409, Length=175, Percent_Identity=27.4285714285714, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_THETN (Q8RDD1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621810.1
- ProteinModelPortal:   Q8RDD1
- GeneID:   996595
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0110
- NMPDR:   fig|273068.3.peg.283
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   TTEN273068:TTE0110-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 73553; Mature: 73553

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVMFLTGTDEHGQKIQRIA
CCCEEEEECCEECCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
REKGMSPKEYVDGIVEWIKDLWKTMDISYDHFIRTTDAYHEEIVQKIFMKLYEQGDIYKG
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EYEGWYCTPCESFWTESQLVDGKCPDCGRPVERVTEEGYFFRLSAYGDKLLKYYEEHPDF
CCCCEEECCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCC
IQPESRRNEMINFIKAGLEDLFVSRSTFDWGIKVPFDPKHVIYVWIDALSNYITALGYMT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHHCCC
ENDEKFKKYWPADVHLVGKEIVRFHTIIWPAMLMALGLPLPKKVFGHGWLILEGGKMSKS
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCC
KGNVVDPKELVDRYGVDAIRYFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTV
CCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEKYFDGVLPKPSSQEEIDEDLINVAQNLPQKVEEYMDKLQFSNALIEIWKLVSRANK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDETMPWVLAKDESKRGRLGTVLYNLAESLRFIGILISPFMPNTPKKMFEQLGITEDLT
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHH
TWESLKFGLLKEGTRVKRGEILFPRIDVEKELASLEKKTEEKTKETKEEKIDYITIEDFS
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
KVQLRVAEILEAEKVEGSDKLIKMKLKVGEEIRQIVGGIGKYYSPEELIGKKIIIVYNLQ
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECC
PRKLMGIESQGMLLAATNEGKMALLTVDKDIESGSKIS
CCCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVMFLTGTDEHGQKIQRIA
CCCEEEEECCEECCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
REKGMSPKEYVDGIVEWIKDLWKTMDISYDHFIRTTDAYHEEIVQKIFMKLYEQGDIYKG
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EYEGWYCTPCESFWTESQLVDGKCPDCGRPVERVTEEGYFFRLSAYGDKLLKYYEEHPDF
CCCCEEECCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCC
IQPESRRNEMINFIKAGLEDLFVSRSTFDWGIKVPFDPKHVIYVWIDALSNYITALGYMT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHHCCC
ENDEKFKKYWPADVHLVGKEIVRFHTIIWPAMLMALGLPLPKKVFGHGWLILEGGKMSKS
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCC
KGNVVDPKELVDRYGVDAIRYFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTV
CCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEKYFDGVLPKPSSQEEIDEDLINVAQNLPQKVEEYMDKLQFSNALIEIWKLVSRANK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDETMPWVLAKDESKRGRLGTVLYNLAESLRFIGILISPFMPNTPKKMFEQLGITEDLT
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHH
TWESLKFGLLKEGTRVKRGEILFPRIDVEKELASLEKKTEEKTKETKEEKIDYITIEDFS
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
KVQLRVAEILEAEKVEGSDKLIKMKLKVGEEIRQIVGGIGKYYSPEELIGKKIIIVYNLQ
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECC
PRKLMGIESQGMLLAATNEGKMALLTVDKDIESGSKIS
CCCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336