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The map label for this gene is LeuC [H]

Identifier: 20806559

GI number: 20806559

Start: 20358

End: 21614

Strand: Direct

Name: LeuC [H]

Synonym: TTE0017

Alternate gene names: 20806559

Gene position: 20358-21614 (Clockwise)

Preceding gene: 20806558

Following gene: 20806560

Centisome position: 0.76

GC content: 42.48

Gene sequence:

>1257_bases
TTGGGCTTAACTCTTACACAAAAGATTTTATCGGCAAAGGCGGGAAGAGAGGTAAAACCTGGAGAATTGATAGAGATAGA
CGTGGATATGGTGCTGGGAAATGATATAACGGCACCTGTTGCCATAAAAGAGTTTGAAAAAATAGGGGTAGATAAGGTTT
TTGACAACACAAAAATAGCTTTAGTGCCAGACCACTTTGTGCCGAGCAAGGATATAAAATCAGCGGAGCAGGTAAATGTA
ATGAGAAAATTTGCTAAAAAATATAATATAGTTAATTTCTTTGAAGTGGGAAGGATGGGCATTGAGCATGCCCTCCTTCC
AGAACAGGGCCTTGTTCTTCCAGGTGATGTGGTGATAGGAGCCGATTCTCATACCTGCACTTACGGTGCTCTTACCTGCT
TTGCTACAGGAGTGGGAAGCACCGATATGGCTGCGGCTATGGCCACAGGGAAAGCCTGGTTTAAGGTGCCTGAGGCAATA
AAGTTTGTTTTAAAAGGAAATCTCGGCAAATGGGTGAGTGGAAAGGACGTGATACTCTATATCATTGGCAAGATTGGAGT
TGACGGGGCTTTATATAAATCCATGGAATTTACAGGGAATATAAAAGCTTTGTCAATTGACGACAGGTTTACAATTGCAA
ATATGGCGATTGAAGCAGGAGCAAAGAATGGAATCTTTGATTTTGACGAGATTACAGAAGCTTATGTTAAAAAGAGGGCA
AAGCGAGAGTATAAAGTTTTTGAAAGGGATGAGGATGCGGAGTACAGCGAAGTAATTGAAATAAATCTTGATGATATAAG
GCCTCAAGTGGCGTTTCCCCATTTGCCTGAGAATACGAGAAGTATTGATGAAGTCGGAAAAGTTAAGATAGATCAAGTAG
TTATAGGGTCCTGTACAAATGGAAGGCTTTCTGACATGGAGATAGCTTACAGGATTTTGAAGGGCAAAAAGGTTCATCCA
GATGTAAGGCTTATAATTTTCCCGGCTACACAGGAGATATACTTGGAATGCGTGAAGAGAGGGTACATTGAAGAATTTAT
AAAGGCAGGAGCGGCAGTTTCAACTCCTACCTGTGGTCCATGCCTTGGAGGGCATATGGGAGTCCTGGCAAAGGGAGAAA
GAGCTTTAGCTACTACAAATAGAAATTTTGTGGGAAGGATGGGACATCCTGAAAGCGAGGTTTATCTCGCAAGTCCAGCA
GTTGCAGCGGCTTCAGCAATAGCTGGCTACATTGTAAGCCCTGAGGAGGTGGAATGA

Upstream 100 bases:

>100_bases
AAGATTTAGCCGATAGGAAAAAGACCATAGTGGAAGAGGATATTCGATTTGTTGTAGAGGAGACTATAGAAGAATTTAGG
GGCTTTAGGGAGGGAGAAGC

Downstream 100 bases:

>100_bases
TGCAGGGCAAAGCTATAAAGTACGGGGACAACATCGACACAGATGTGATAATACCGGCTAGATTTTTAAATACCTCCGAT
CCGAAAGAATTAGCAGAACA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGLTLTQKILSAKAGREVKPGELIEIDVDMVLGNDITAPVAIKEFEKIGVDKVFDNTKIALVPDHFVPSKDIKSAEQVNV
MRKFAKKYNIVNFFEVGRMGIEHALLPEQGLVLPGDVVIGADSHTCTYGALTCFATGVGSTDMAAAMATGKAWFKVPEAI
KFVLKGNLGKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKKRA
KREYKVFERDEDAEYSEVIEINLDDIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYRILKGKKVHP
DVRLIIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGVLAKGERALATTNRNFVGRMGHPESEVYLASPA
VAAASAIAGYIVSPEEVE

Sequences:

>Translated_418_residues
MGLTLTQKILSAKAGREVKPGELIEIDVDMVLGNDITAPVAIKEFEKIGVDKVFDNTKIALVPDHFVPSKDIKSAEQVNV
MRKFAKKYNIVNFFEVGRMGIEHALLPEQGLVLPGDVVIGADSHTCTYGALTCFATGVGSTDMAAAMATGKAWFKVPEAI
KFVLKGNLGKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKKRA
KREYKVFERDEDAEYSEVIEINLDDIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYRILKGKKVHP
DVRLIIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGVLAKGERALATTNRNFVGRMGHPESEVYLASPA
VAAASAIAGYIVSPEEVE
>Mature_417_residues
GLTLTQKILSAKAGREVKPGELIEIDVDMVLGNDITAPVAIKEFEKIGVDKVFDNTKIALVPDHFVPSKDIKSAEQVNVM
RKFAKKYNIVNFFEVGRMGIEHALLPEQGLVLPGDVVIGADSHTCTYGALTCFATGVGSTDMAAAMATGKAWFKVPEAIK
FVLKGNLGKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKKRAK
REYKVFERDEDAEYSEVIEINLDDIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYRILKGKKVHPD
VRLIIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGVLAKGERALATTNRNFVGRMGHPESEVYLASPAV
AAASAIAGYIVSPEEVE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=454, Percent_Identity=31.4977973568282, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=26.8929503916449, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI8659555, Length=377, Percent_Identity=25.1989389920424, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=31.317494600432, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI87081781, Length=347, Percent_Identity=26.5129682997118, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI2367097, Length=467, Percent_Identity=26.7665952890792, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=30.1339285714286, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=30.6569343065693, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=31.2056737588652, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=376, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=34.8888888888889, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=31.2896405919662, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=30.1339285714286, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322261, Length=458, Percent_Identity=27.5109170305677, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI281365315, Length=449, Percent_Identity=30.2895322939866, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864292, Length=449, Percent_Identity=30.2895322939866, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=30.3571428571429, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI161076999, Length=412, Percent_Identity=30.8252427184466, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17137564, Length=404, Percent_Identity=25.4950495049505, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24645686, Length=398, Percent_Identity=25.1256281407035, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45511; Mature: 45380

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTLTQKILSAKAGREVKPGELIEIDVDMVLGNDITAPVAIKEFEKIGVDKVFDNTKIA
CCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCCHHHCCCCEEE
LVPDHFVPSKDIKSAEQVNVMRKFAKKYNIVNFFEVGRMGIEHALLPEQGLVLPGDVVIG
EECCCCCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHCCCCCCEECCCCEEEE
ADSHTCTYGALTCFATGVGSTDMAAAMATGKAWFKVPEAIKFVLKGNLGKWVSGKDVILY
CCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKKRA
EEEHHCCCHHHHHCEEECCCEEEEEECCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHH
KREYKVFERDEDAEYSEVIEINLDDIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTN
HHHHHHHCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEEECCCC
GRLSDMEIAYRILKGKKVHPDVRLIIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGP
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CLGGHMGVLAKGERALATTNRNFVGRMGHPESEVYLASPAVAAASAIAGYIVSPEEVE
CCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEEECCHHHHHHHHHHEECCHHCCC
>Mature Secondary Structure 
GLTLTQKILSAKAGREVKPGELIEIDVDMVLGNDITAPVAIKEFEKIGVDKVFDNTKIA
CCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCCHHHCCCCEEE
LVPDHFVPSKDIKSAEQVNVMRKFAKKYNIVNFFEVGRMGIEHALLPEQGLVLPGDVVIG
EECCCCCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHCCCCCCEECCCCEEEE
ADSHTCTYGALTCFATGVGSTDMAAAMATGKAWFKVPEAIKFVLKGNLGKWVSGKDVILY
CCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKKRA
EEEHHCCCHHHHHCEEECCCEEEEEECCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHH
KREYKVFERDEDAEYSEVIEINLDDIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTN
HHHHHHHCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEEECCCC
GRLSDMEIAYRILKGKKVHPDVRLIIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGP
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CLGGHMGVLAKGERALATTNRNFVGRMGHPESEVYLASPAVAAASAIAGYIVSPEEVE
CCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEEECCHHHHHHHHHHEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA