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The map label for this gene is leuD

Identifier: 20806560

GI number: 20806560

Start: 21614

End: 22099

Strand: Direct

Name: leuD

Synonym: TTE0018

Alternate gene names: 20806560

Gene position: 21614-22099 (Clockwise)

Preceding gene: 20806559

Following gene: 20806561

Centisome position: 0.8

GC content: 38.27

Gene sequence:

>486_bases
ATGCAGGGCAAAGCTATAAAGTACGGGGACAACATCGACACAGATGTGATAATACCGGCTAGATTTTTAAATACCTCCGA
TCCGAAAGAATTAGCAGAACACTGCATGGAGGATCTGGATAGGGAGTTTAAAAATAAGGTAAAAGAAGGGGATATATTAG
TCGTAGGAGAAAATTTTGGCTGCGGTTCCTCAAGGGAGCACGCTCCTCTTGCCATTAAAGCTTCTGGAATTTCATGTATA
ATTGCCAAGTCTTTTGCCAGGATTTTTTACAGAAATGCCATAAATATAGGACTTCCTATACTGGAATCTAGAGAAGCTGT
GGATGGCATTGAAGAAGGGGATATAGTTTCTGTGGATGTAGATAATGGAATAATTAGAAATGTCACAAAAGGGACAGAAT
TTAAAGCACAGCCATTTCCGGAATTTATAAAAGAGATAATAAAATATGGAGGACTTATAAATTACGTGAGAGAGAAGGTG
CGTTAA

Upstream 100 bases:

>100_bases
GGATGGGACATCCTGAAAGCGAGGTTTATCTCGCAAGTCCAGCAGTTGCAGCGGCTTCAGCAATAGCTGGCTACATTGTA
AGCCCTGAGGAGGTGGAATG

Downstream 100 bases:

>100_bases
TGTACAGGATAGCAGTTTTGCCAGGAGATGGGATAGGTCCAGAAGTGATAGAAGAAGGTTTAAAGGTTTTAAAAGCTGTG
GAAGAAAAGTACGGGCTCAG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPKELAEHCMEDLDREFKNKVKEGDILVVGENFGCGSSREHAPLAIKASGISCI
IAKSFARIFYRNAINIGLPILESREAVDGIEEGDIVSVDVDNGIIRNVTKGTEFKAQPFPEFIKEIIKYGGLINYVREKV
R

Sequences:

>Translated_161_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPKELAEHCMEDLDREFKNKVKEGDILVVGENFGCGSSREHAPLAIKASGISCI
IAKSFARIFYRNAINIGLPILESREAVDGIEEGDIVSVDVDNGIIRNVTKGTEFKAQPFPEFIKEIIKYGGLINYVREKV
R
>Mature_161_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPKELAEHCMEDLDREFKNKVKEGDILVVGENFGCGSSREHAPLAIKASGISCI
IAKSFARIFYRNAINIGLPILESREAVDGIEEGDIVSVDVDNGIIRNVTKGTEFKAQPFPEFIKEIIKYGGLINYVREKV
R

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=112, Percent_Identity=40.1785714285714, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=174, Percent_Identity=37.3563218390805, Blast_Score=94, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=44.6601941747573, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_THETN (Q8RDK1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621731.1
- ProteinModelPortal:   Q8RDK1
- SMR:   Q8RDK1
- GeneID:   996501
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0018
- NMPDR:   fig|273068.3.peg.29
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   PRK00439
- BioCyc:   TTEN273068:TTE0018-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02084
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17889; Mature: 17889

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKAIKYGDNIDTDVIIPARFLNTSDPKELAEHCMEDLDREFKNKVKEGDILVVGENFG
CCCCEEEECCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
CGSSREHAPLAIKASGISCIIAKSFARIFYRNAINIGLPILESREAVDGIEEGDIVSVDV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEC
DNGIIRNVTKGTEFKAQPFPEFIKEIIKYGGLINYVREKVR
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MQGKAIKYGDNIDTDVIIPARFLNTSDPKELAEHCMEDLDREFKNKVKEGDILVVGENFG
CCCCEEEECCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
CGSSREHAPLAIKASGISCIIAKSFARIFYRNAINIGLPILESREAVDGIEEGDIVSVDV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEC
DNGIIRNVTKGTEFKAQPFPEFIKEIIKYGGLINYVREKVR
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336