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The map label for this gene is LeuA

Identifier: 20806558

GI number: 20806558

Start: 19174

End: 20367

Strand: Direct

Name: LeuA

Synonym: TTE0016

Alternate gene names: 20806558

Gene position: 19174-20367 (Clockwise)

Preceding gene: 20806557

Following gene: 20806559

Centisome position: 0.71

GC content: 41.12

Gene sequence:

>1194_bases
ATGGGGGTTAAGAGAGTTATAGTTTTTGACACTACTTTAAGAGATGGTGAACAGACGCCAGGGGTAAATTTTAACAGCAG
GGATAAGCTTGAAATTGCCTATCAGCTGGCAAAGCTTGGGGTAGACGTGATAGAAGCGGGATTTCCTGCAGCTTCCAATG
GGGATTTTGAGGCAGTAAAGAATATTGCTGACTACGTAAAAGGCGTAACCATTGCTGCGATGGGAAGAGCTGTGAAAGAG
GACATTGACAGGGCCAGCAGTGCTCTTAAAAATGCAGAAAAGTCCAGATTGCACGTCTTTATAGCTACTTCTGATATACA
CCTTCAGTACAAGTTGAAGATGACAAGAGATGAGGTTCTGAAAAAAGCAGTTGAAATGGTGAAATACGCTAGAGGGAAGT
TTGATGAGATAGAGTTTTCAGCAGAGGATGCCTCAAGGACTGATTGGGACTTTTTAGTGAAAGTGTTTAGCGAAGTAATT
GATGCAGGAGCCCATATTATAAATGTGCCTGATACGGTAGGTTATGCTATGCCGAGGGAGTATGGAGAGCTTATAAGGTA
TATAAGAAACAATGTTCCAAATATTGATGGAGTAACAATAAGTGCGCATTGCCACAATGATTTAGGGCTGGCTGTAGCCA
ATTCTCTTTCTGCTATAGAAAATGGAGCAACTCAAGTAGAGGTTACTGTGAACGGGATAGGAGAGAGGGCAGGAAATGCG
GCTATGGAAGAGGTGATAATGGCTTTAAATACCAGAAAAGATTATTTTGGACTTGTACACGGCATAAATACAAAAGAGAT
ATACAATACGAGCAAACTAGTTAGCGAACTCACAGGGATTAAGTTACAGCCAAATAAAGCTATAGTAGGAGCTAATGCTT
TCAGGCACCAGTCCGGGATACACCAGCATGGAGTCATAAACAATAGATTAACTTATGAAATAATGAGGCCAGAGGATATA
GGAGTTGTTCCTGACACTTTCGCTTTAGGTAAACTTTCAGGAAGAAACGCTTTTGAACTTAAAGTAAGGCAACTGGGCTA
CAACCTTTCTCCCGGTGAGATAAGTGAAGCTTTTAGAAAGTTTAAAGATTTAGCCGATAGGAAAAAGACCATAGTGGAAG
AGGATATTCGATTTGTTGTAGAGGAGACTATAGAAGAATTTAGGGGCTTTAGGGAGGGAGAAGCTTGGGCTTAA

Upstream 100 bases:

>100_bases
TAAAGAAAGAGAAGCAAACCATCCAATTGAAAAAGTTGGAAAAAGTTTAAGGGAAATGATGCCATGGCTTAGTAAAAAAT
AAAAAGGGGGTAGGTTAAAA

Downstream 100 bases:

>100_bases
CTCTTACACAAAAGATTTTATCGGCAAAGGCGGGAAGAGAGGTAAAACCTGGAGAATTGATAGAGATAGACGTGGATATG
GTGCTGGGAAATGATATAAC

Product: isopropylmalate/homocitrate/citramalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MGVKRVIVFDTTLRDGEQTPGVNFNSRDKLEIAYQLAKLGVDVIEAGFPAASNGDFEAVKNIADYVKGVTIAAMGRAVKE
DIDRASSALKNAEKSRLHVFIATSDIHLQYKLKMTRDEVLKKAVEMVKYARGKFDEIEFSAEDASRTDWDFLVKVFSEVI
DAGAHIINVPDTVGYAMPREYGELIRYIRNNVPNIDGVTISAHCHNDLGLAVANSLSAIENGATQVEVTVNGIGERAGNA
AMEEVIMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQSGIHQHGVINNRLTYEIMRPEDI
GVVPDTFALGKLSGRNAFELKVRQLGYNLSPGEISEAFRKFKDLADRKKTIVEEDIRFVVEETIEEFRGFREGEAWA

Sequences:

>Translated_397_residues
MGVKRVIVFDTTLRDGEQTPGVNFNSRDKLEIAYQLAKLGVDVIEAGFPAASNGDFEAVKNIADYVKGVTIAAMGRAVKE
DIDRASSALKNAEKSRLHVFIATSDIHLQYKLKMTRDEVLKKAVEMVKYARGKFDEIEFSAEDASRTDWDFLVKVFSEVI
DAGAHIINVPDTVGYAMPREYGELIRYIRNNVPNIDGVTISAHCHNDLGLAVANSLSAIENGATQVEVTVNGIGERAGNA
AMEEVIMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQSGIHQHGVINNRLTYEIMRPEDI
GVVPDTFALGKLSGRNAFELKVRQLGYNLSPGEISEAFRKFKDLADRKKTIVEEDIRFVVEETIEEFRGFREGEAWA
>Mature_396_residues
GVKRVIVFDTTLRDGEQTPGVNFNSRDKLEIAYQLAKLGVDVIEAGFPAASNGDFEAVKNIADYVKGVTIAAMGRAVKED
IDRASSALKNAEKSRLHVFIATSDIHLQYKLKMTRDEVLKKAVEMVKYARGKFDEIEFSAEDASRTDWDFLVKVFSEVID
AGAHIINVPDTVGYAMPREYGELIRYIRNNVPNIDGVTISAHCHNDLGLAVANSLSAIENGATQVEVTVNGIGERAGNAA
MEEVIMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQSGIHQHGVINNRLTYEIMRPEDIG
VVPDTFALGKLSGRNAFELKVRQLGYNLSPGEISEAFRKFKDLADRKKTIVEEDIRFVVEETIEEFRGFREGEAWA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=390, Percent_Identity=48.2051282051282, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6320071, Length=394, Percent_Identity=32.741116751269, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320019, Length=389, Percent_Identity=32.6478149100257, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6324682, Length=348, Percent_Identity=30.7471264367816, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=348, Percent_Identity=30.1724137931034, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_THETN (Q8RDK3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621729.1
- ProteinModelPortal:   Q8RDK3
- SMR:   Q8RDK3
- GeneID:   998031
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0016
- NMPDR:   fig|273068.3.peg.10
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   CLSK900578
- BioCyc:   TTEN273068:TTE0016-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 44027; Mature: 43895

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKRVIVFDTTLRDGEQTPGVNFNSRDKLEIAYQLAKLGVDVIEAGFPAASNGDFEAVK
CCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHH
NIADYVKGVTIAAMGRAVKEDIDRASSALKNAEKSRLHVFIATSDIHLQYKLKMTRDEVL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEECCHHHHH
KKAVEMVKYARGKFDEIEFSAEDASRTDWDFLVKVFSEVIDAGAHIINVPDTVGYAMPRE
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHCCCCCHH
YGELIRYIRNNVPNIDGVTISAHCHNDLGLAVANSLSAIENGATQVEVTVNGIGERAGNA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHH
AMEEVIMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQSGI
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHHHCCC
HQHGVINNRLTYEIMRPEDIGVVPDTFALGKLSGRNAFELKVRQLGYNLSPGEISEAFRK
CCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCHHEEEHHHHCCCCCCHHHHHHHHH
FKDLADRKKTIVEEDIRFVVEETIEEFRGFREGEAWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GVKRVIVFDTTLRDGEQTPGVNFNSRDKLEIAYQLAKLGVDVIEAGFPAASNGDFEAVK
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHH
NIADYVKGVTIAAMGRAVKEDIDRASSALKNAEKSRLHVFIATSDIHLQYKLKMTRDEVL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEECCHHHHH
KKAVEMVKYARGKFDEIEFSAEDASRTDWDFLVKVFSEVIDAGAHIINVPDTVGYAMPRE
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHCCCCCHH
YGELIRYIRNNVPNIDGVTISAHCHNDLGLAVANSLSAIENGATQVEVTVNGIGERAGNA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHH
AMEEVIMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQSGI
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHHHCCC
HQHGVINNRLTYEIMRPEDIGVVPDTFALGKLSGRNAFELKVRQLGYNLSPGEISEAFRK
CCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCHHEEEHHHHCCCCCCHHHHHHHHH
FKDLADRKKTIVEEDIRFVVEETIEEFRGFREGEAWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336