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The map label for this gene is InfB_2

Identifier: 20095031

GI number: 20095031

Start: 1604190

End: 1605986

Strand: Direct

Name: InfB_2

Synonym: MK1595

Alternate gene names: 20095031

Gene position: 1604190-1605986 (Clockwise)

Preceding gene: 20095027

Following gene: 20095034

Centisome position: 94.64

GC content: 58.99

Gene sequence:

>1797_bases
TTGTCCGAGAGTAATAAAGCCATACGACAACCGATAATTTCGGTACTTGGGCACGTTGACCATGGGAAGACCACCCTCCT
AGATAAGATCCGCGGTACCGCCGTAGCGGCGAAGGAGGCCGGGGGTATCACCCAGCACATCGGGGCTTCGGAGATCCCAC
TGGAAGTTGTCAAAGAGATCTGTGGACCGTTGCTCGAACAGCTGGATGTGGAGATCACGATACCCGGACTATTGTTTATC
GATACCCCGGGTCATGAAGCTTTCACTAACCTTCGAAGGCGTGGAGGAGCACTAGCCGACATAGCGATCCTAGTGATCGA
CATCATGGAAGGCGTGATGCCACAGACTGAAGAGGCCCTTAGGATACTCAGGAGGTACCGCACCCCGTTCGTCGTCGCCG
CCAACAAGGTGGACAGGGTGCCGGGCTGGAAGTCCCACGAGGACACTCCTTTCCTCGAGTCGTTCCAGAAGCAGTCCCCG
GAGGTACAGCAGCGGCTGGAAGAAAAAGTCTACGAGCTCATCGGACAGCTCCACCAGCATGGGTTCCAGGCGGAACGCTT
CGATAGGGTGAGGGACTTCACGAGAACAGTCGCGATAGTTCCCACGAGCGGGGTCACAGGGGAAGGGATTCCGGAGCTGC
TGATGGTCGTGACCGGACTGGCACAGCGCTTCCTGGAAGAGCAGCTGAAAATAGAGGTGGAAGGTCCCGGAAAGGCCGCG
ATCCTGGAGGTCAAGGAGGAACCGGGACTCGGCCACACGGTCGACGCAATCCTCTACGACGGTATCATCCGAACGGGCGA
CACGATAGTCATCGGTCATCCAGAAGAGCCGATCGTCACCCGGGTGAGATCCCTGCTGAAGCCGAAGCCTCTGGACGAGA
TGCGCGACCCGAGCGACCGATTCCGTAAGGTGGATGAGGTGACGGCCGCCGCCGGTGTGAAGATCAGTGCCCCGGAGCTC
GAGGAGGCCGTGGCCGGCGCCCCGTTACGCGTTGTCGGGGAAGACGAGGACGTCGAGGAAGTCGTGCGCGAGGTCCAAGA
GGAGGTCGAGGAGGTCACCATCGAGACCGACCAAGAGGGAATCATCATCAAGGCCGACACCCTGGGAACCCTGGAGGCCG
TCGTCGGGGAGTTCAAGGAAAAGGACGTTCCGATCCGCAAGGCCGACGTCGGAGACATCACCAAGAAGGACGTCATCGAG
GCGCACGCGGTCGCGGAGAAGGATCCGCTACTCGGCGTGATCGTAGGGTTCAACGTAGGGGTCACCGAAGAGGCGCGGGA
GCTGGCCGACGAGTACGACGTCGACATCATCATCGACGACGTGATCTACGAGCTAGTCGAGAAGTACGAGGAGATGGTCG
AAAAGCGGATCGAGCGAGAGCGGCGCAAGCGCCTGGACGAACTCGTACGTCCCGGGAAGATAAAGGTGCTCCCCGGGTAC
ATCTTCCGCCAGAGTAAGCCGGCGATCGTCGGCGTTCAGGTCTTGGCGGGTGTGATCAAGCCCGGGTATCCACTGATGCG
AGAGGATGGTAGGGAACTCGGGGAGATCAAGCAGATCCAGATGCACGGTGAACCGATTAAAGAGGCGAAGAAGGGACAGG
AAGTGGCCATATCCATCGAGGGTCCCATCGTCGGTAGACACTTCGAGGAGGGCGATATCCTGTATACGGACGTACCTTCC
GAGCACGCGAAGCTCATGTTCGAGGAGTTCAAGGATCTCCTGACGGAGGACGAGTTGGAGGCCCTTAAAGAGATAGCCGA
GATCAAACGCAAGGAAGACCCGTTCTACGGGATGTAA

Upstream 100 bases:

>100_bases
TATTGCCCGTTGCAAATAACACTTTCATATTCCATCCACCCAAGGTTTAAATTCGAGTAAAATGGCGTTTCGACGAAAAA
TCTCAAGGGAGTGATATAAG

Downstream 100 bases:

>100_bases
CCTCGATACCGCCGATGACGACAAACCTGGGCTCGGATCCCGTGAAGAGCCGTGTCCTAGCCGAGGCGGCGTCGCGGCAC
TATCGCCGGGAGAACGGGTA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSESNKAIRQPIISVLGHVDHGKTTLLDKIRGTAVAAKEAGGITQHIGASEIPLEVVKEICGPLLEQLDVEITIPGLLFI
DTPGHEAFTNLRRRGGALADIAILVIDIMEGVMPQTEEALRILRRYRTPFVVAANKVDRVPGWKSHEDTPFLESFQKQSP
EVQQRLEEKVYELIGQLHQHGFQAERFDRVRDFTRTVAIVPTSGVTGEGIPELLMVVTGLAQRFLEEQLKIEVEGPGKAA
ILEVKEEPGLGHTVDAILYDGIIRTGDTIVIGHPEEPIVTRVRSLLKPKPLDEMRDPSDRFRKVDEVTAAAGVKISAPEL
EEAVAGAPLRVVGEDEDVEEVVREVQEEVEEVTIETDQEGIIIKADTLGTLEAVVGEFKEKDVPIRKADVGDITKKDVIE
AHAVAEKDPLLGVIVGFNVGVTEEARELADEYDVDIIIDDVIYELVEKYEEMVEKRIERERRKRLDELVRPGKIKVLPGY
IFRQSKPAIVGVQVLAGVIKPGYPLMREDGRELGEIKQIQMHGEPIKEAKKGQEVAISIEGPIVGRHFEEGDILYTDVPS
EHAKLMFEEFKDLLTEDELEALKEIAEIKRKEDPFYGM

Sequences:

>Translated_598_residues
MSESNKAIRQPIISVLGHVDHGKTTLLDKIRGTAVAAKEAGGITQHIGASEIPLEVVKEICGPLLEQLDVEITIPGLLFI
DTPGHEAFTNLRRRGGALADIAILVIDIMEGVMPQTEEALRILRRYRTPFVVAANKVDRVPGWKSHEDTPFLESFQKQSP
EVQQRLEEKVYELIGQLHQHGFQAERFDRVRDFTRTVAIVPTSGVTGEGIPELLMVVTGLAQRFLEEQLKIEVEGPGKAA
ILEVKEEPGLGHTVDAILYDGIIRTGDTIVIGHPEEPIVTRVRSLLKPKPLDEMRDPSDRFRKVDEVTAAAGVKISAPEL
EEAVAGAPLRVVGEDEDVEEVVREVQEEVEEVTIETDQEGIIIKADTLGTLEAVVGEFKEKDVPIRKADVGDITKKDVIE
AHAVAEKDPLLGVIVGFNVGVTEEARELADEYDVDIIIDDVIYELVEKYEEMVEKRIERERRKRLDELVRPGKIKVLPGY
IFRQSKPAIVGVQVLAGVIKPGYPLMREDGRELGEIKQIQMHGEPIKEAKKGQEVAISIEGPIVGRHFEEGDILYTDVPS
EHAKLMFEEFKDLLTEDELEALKEIAEIKRKEDPFYGM
>Mature_597_residues
SESNKAIRQPIISVLGHVDHGKTTLLDKIRGTAVAAKEAGGITQHIGASEIPLEVVKEICGPLLEQLDVEITIPGLLFID
TPGHEAFTNLRRRGGALADIAILVIDIMEGVMPQTEEALRILRRYRTPFVVAANKVDRVPGWKSHEDTPFLESFQKQSPE
VQQRLEEKVYELIGQLHQHGFQAERFDRVRDFTRTVAIVPTSGVTGEGIPELLMVVTGLAQRFLEEQLKIEVEGPGKAAI
LEVKEEPGLGHTVDAILYDGIIRTGDTIVIGHPEEPIVTRVRSLLKPKPLDEMRDPSDRFRKVDEVTAAAGVKISAPELE
EAVAGAPLRVVGEDEDVEEVVREVQEEVEEVTIETDQEGIIIKADTLGTLEAVVGEFKEKDVPIRKADVGDITKKDVIEA
HAVAEKDPLLGVIVGFNVGVTEEARELADEYDVDIIIDDVIYELVEKYEEMVEKRIERERRKRLDELVRPGKIKVLPGYI
FRQSKPAIVGVQVLAGVIKPGYPLMREDGRELGEIKQIQMHGEPIKEAKKGQEVAISIEGPIVGRHFEEGDILYTDVPSE
HAKLMFEEFKDLLTEDELEALKEIAEIKRKEDPFYGM

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=597, Percent_Identity=38.358458961474, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI53729339, Length=264, Percent_Identity=32.9545454545455, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI53729337, Length=264, Percent_Identity=32.9545454545455, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1789559, Length=561, Percent_Identity=28.3422459893048, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI2367247, Length=133, Percent_Identity=39.0977443609023, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI212656558, Length=601, Percent_Identity=36.6056572379368, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71994658, Length=131, Percent_Identity=41.9847328244275, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319282, Length=604, Percent_Identity=39.0728476821192, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324550, Length=603, Percent_Identity=25.0414593698176, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24656849, Length=595, Percent_Identity=36.4705882352941, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28572034, Length=265, Percent_Identity=32.0754716981132, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI160714833, Length=87, Percent_Identity=50.5747126436782, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_METKA (Q8TV06)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614878.1
- ProteinModelPortal:   Q8TV06
- SMR:   Q8TV06
- GeneID:   1478190
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1595
- NMPDR:   fig|190192.1.peg.1591
- HOGENOM:   HBG635083
- OMA:   MGLSQRY
- ProtClustDB:   PRK04004
- BioCyc:   MKAN190192:MK1595-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66606; Mature: 66475

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNKAIRQPIISVLGHVDHGKTTLLDKIRGTAVAAKEAGGITQHIGASEIPLEVVKEI
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEHHHCCCCHHHCCCCCCCHHHHHHH
CGPLLEQLDVEITIPGLLFIDTPGHEAFTNLRRRGGALADIAILVIDIMEGVMPQTEEAL
HHHHHHHCCCEEEECCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
RILRRYRTPFVVAANKVDRVPGWKSHEDTPFLESFQKQSPEVQQRLEEKVYELIGQLHQH
HHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GFQAERFDRVRDFTRTVAIVPTSGVTGEGIPELLMVVTGLAQRFLEEQLKIEVEGPGKAA
CCCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
ILEVKEEPGLGHTVDAILYDGIIRTGDTIVIGHPEEPIVTRVRSLLKPKPLDEMRDPSDR
EEEEECCCCCCHHHHHHHHHCHHCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCCHHH
FRKVDEVTAAAGVKISAPELEEAVAGAPLRVVGEDEDVEEVVREVQEEVEEVTIETDQEG
HHHHHHHHHHCCCEECCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCCC
IIIKADTLGTLEAVVGEFKEKDVPIRKADVGDITKKDVIEAHAVAEKDPLLGVIVGFNVG
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
VTEEARELADEYDVDIIIDDVIYELVEKYEEMVEKRIERERRKRLDELVRPGKIKVLPGY
CCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
IFRQSKPAIVGVQVLAGVIKPGYPLMREDGRELGEIKQIQMHGEPIKEAKKGQEVAISIE
HHCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEEC
GPIVGRHFEEGDILYTDVPSEHAKLMFEEFKDLLTEDELEALKEIAEIKRKEDPFYGM
CCEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SESNKAIRQPIISVLGHVDHGKTTLLDKIRGTAVAAKEAGGITQHIGASEIPLEVVKEI
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEHHHCCCCHHHCCCCCCCHHHHHHH
CGPLLEQLDVEITIPGLLFIDTPGHEAFTNLRRRGGALADIAILVIDIMEGVMPQTEEAL
HHHHHHHCCCEEEECCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
RILRRYRTPFVVAANKVDRVPGWKSHEDTPFLESFQKQSPEVQQRLEEKVYELIGQLHQH
HHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GFQAERFDRVRDFTRTVAIVPTSGVTGEGIPELLMVVTGLAQRFLEEQLKIEVEGPGKAA
CCCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
ILEVKEEPGLGHTVDAILYDGIIRTGDTIVIGHPEEPIVTRVRSLLKPKPLDEMRDPSDR
EEEEECCCCCCHHHHHHHHHCHHCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCCHHH
FRKVDEVTAAAGVKISAPELEEAVAGAPLRVVGEDEDVEEVVREVQEEVEEVTIETDQEG
HHHHHHHHHHCCCEECCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCCC
IIIKADTLGTLEAVVGEFKEKDVPIRKADVGDITKKDVIEAHAVAEKDPLLGVIVGFNVG
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
VTEEARELADEYDVDIIIDDVIYELVEKYEEMVEKRIERERRKRLDELVRPGKIKVLPGY
CCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
IFRQSKPAIVGVQVLAGVIKPGYPLMREDGRELGEIKQIQMHGEPIKEAKKGQEVAISIE
HHCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEEC
GPIVGRHFEEGDILYTDVPSEHAKLMFEEFKDLLTEDELEALKEIAEIKRKEDPFYGM
CCEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014