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The map label for this gene is Ndk

Identifier: 20095027

GI number: 20095027

Start: 1601232

End: 1601696

Strand: Direct

Name: Ndk

Synonym: MK1591

Alternate gene names: 20095027

Gene position: 1601232-1601696 (Clockwise)

Preceding gene: 20095026

Following gene: 20095031

Centisome position: 94.47

GC content: 59.57

Gene sequence:

>465_bases
TTGGCGGAGGTTCAGAGGGAGTTCGTTATGATCAAGCCGGACGGCGTGGTCAGGGGTCTGGTCGGAGAGGTGATCCGACG
TCTCGAGCGCAAGGGTCTCAAGATAGTCGCGCTGGAGATGCGTCAGCTCGACCGCGAGACGGCGGAGAAGCTCTACGAAG
AACACCGAGACAAGCCATTCTTCGAGGATCTCATCGAGTACGTGACATCCGGCCCCGTCGTGGTGATGATAGTCGAAGGC
CGGAAGGCCGTGAAGGTCGTGAGGAACATTATCGGCGCGACGGATCCCGCCGAAGCTGCACCGGGTACGGTCCGCGGAGA
TTTCGCGCTGGATATCGGCAGGAACGTGGTACACGCCTCCGACTCTCCGGAGTCCGCCGAGCGGGAGATCGAGATAGTGT
TCGGCAAGGACCTATCCACGATAGATTACGAACGATGCGACGAGGAGTGGCTGTACGAATCATAG

Upstream 100 bases:

>100_bases
AAACGTCGATCTACCGGAACCCGGACCGGATACTCCCATCGTCAGGATCGTCCGCAAAGGTGATTAGACTGAAGGCGGAC
GCGGCGCGGGGGTTCGTCCG

Downstream 100 bases:

>100_bases
GAGCTTCTCGAGCTCTAGAAGGAACTCCTCCAGCCGATCTTCGGGGACTTCGGCACCCGCAGCCCTCTCGTGTCCCCCTC
CTTCTCCATCCACGCGATCA

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MAEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPFFEDLIEYVTSGPVVVMIVEG
RKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHASDSPESAEREIEIVFGKDLSTIDYERCDEEWLYES

Sequences:

>Translated_154_residues
MAEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPFFEDLIEYVTSGPVVVMIVEG
RKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHASDSPESAEREIEIVFGKDLSTIDYERCDEEWLYES
>Mature_153_residues
AEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPFFEDLIEYVTSGPVVVMIVEGR
KAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHASDSPESAEREIEIVFGKDLSTIDYERCDEEWLYES

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI38045913, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=2e-41,
Organism=Homo sapiens, GI66392227, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=2e-41,
Organism=Homo sapiens, GI66392205, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=2e-41,
Organism=Homo sapiens, GI4505409, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=2e-41,
Organism=Homo sapiens, GI66392192, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=2e-41,
Organism=Homo sapiens, GI66392203, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=3e-41,
Organism=Homo sapiens, GI4557797, Length=153, Percent_Identity=47.7124183006536, Blast_Score=164, Evalue=3e-41,
Organism=Homo sapiens, GI37693993, Length=149, Percent_Identity=48.993288590604, Blast_Score=155, Evalue=9e-39,
Organism=Homo sapiens, GI4826862, Length=130, Percent_Identity=52.3076923076923, Blast_Score=144, Evalue=4e-35,
Organism=Homo sapiens, GI7019465, Length=133, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=8e-17,
Organism=Homo sapiens, GI37574614, Length=133, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=9e-17,
Organism=Homo sapiens, GI5031951, Length=136, Percent_Identity=33.8235294117647, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI4505413, Length=134, Percent_Identity=32.089552238806, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI30023828, Length=134, Percent_Identity=29.1044776119403, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788866, Length=133, Percent_Identity=45.8646616541353, Blast_Score=132, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17506807, Length=154, Percent_Identity=47.4025974025974, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71995094, Length=129, Percent_Identity=31.0077519379845, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322783, Length=152, Percent_Identity=45.3947368421053, Blast_Score=158, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45549037, Length=153, Percent_Identity=48.3660130718954, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI18860097, Length=130, Percent_Identity=29.2307692307692, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_METKA (Q8TV10)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614874.1
- ProteinModelPortal:   Q8TV10
- SMR:   Q8TV10
- GeneID:   1478186
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1591
- NMPDR:   fig|190192.1.peg.1587
- HOGENOM:   HBG445152
- OMA:   REIELFF
- BioCyc:   MKAN190192:MK1591-MONOMER
- BRENDA:   2.7.4.6
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 17449; Mature: 17318

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 118-118 BINDING 12-12 BINDING 60-60 BINDING 88-88 BINDING 94-94 BINDING 105-105 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPF
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCH
FEDLIEYVTSGPVVVMIVEGRKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHAS
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEECC
DSPESAEREIEIVFGKDLSTIDYERCDEEWLYES
CCCCCCCCEEEEEECCCCCCCCHHHCCHHHCCCC
>Mature Secondary Structure 
AEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPF
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCH
FEDLIEYVTSGPVVVMIVEGRKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHAS
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEECC
DSPESAEREIEIVFGKDLSTIDYERCDEEWLYES
CCCCCCCCEEEEEECCCCCCCCHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014