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The map label for this gene is thyA

Identifier: 20094806

GI number: 20094806

Start: 1398408

End: 1399037

Strand: Reverse

Name: thyA

Synonym: MK1370

Alternate gene names: 20094806

Gene position: 1399037-1398408 (Counterclockwise)

Preceding gene: 20094807

Following gene: 20094805

Centisome position: 82.54

GC content: 62.54

Gene sequence:

>630_bases
GTGCTGGGTAAGGGCAGACCGTTGGTCGTACGCGGGCGCACCGTGGAGCAGGTGTGGCGGCAGGCCGTCACCGGGATCAA
GGTACACGGCGAGAAGGTGGAGAGGGAGCGGGGTCCGGTCAAGGAAGTACGCGGCCTGATAGCACACCTCGAGCCCTCCG
GACCGGAATCCTTCGATATCCCGGACGATTACCCCCTCGACGAGCACTCCGTGCGGGCGTACGAGGATCAGCTGCTCGAT
CCCGAGCTACGGGGTTTCGAGTACACCTACGGTCATCGGCTCAGGCGGTACTTCGGCCTGGACCAAGTGACCAAGATAGT
GGAAAGGCTCTCTGAATCTAATAACACTAGGAGAGCTATCGCCGTAACCTGGGATCCACGCCGGGATCTCGACGAGGAAG
AGGTACCCTGCCTCACCGCACTCCAACTGCAGTCCGACGGAGGTAGTGGTCTCGAGCTCCACGCGTTCTACCGCTCGTGG
GACGTGGGGAAGGCGCTGGTCGCGAACATGATCGCCCTGAGAAGGTTACAAGAGCACGTGGCGGAGCGGGCGGGGCTGGA
ACCGACGACGTTGACGGTGTATGCCGCCAACGCACACGTGTACGAGGAGGACCTCCCGGACTTACCCTGA

Upstream 100 bases:

>100_bases
GGTGAGTAACTCCCCCGCTCGGAAACGCGGGTTCCCATCAGGTACGGCGGGTACTGGTTAGATTTAACGGACCGCCTCCG
CGTCCGACCGGGGCGCCGAC

Downstream 100 bases:

>100_bases
AGTCATCCGGCCCTCGCGGCCTTCGGCGGCTAGTTCTGCCGGAGCCTCCGACCCATCCGCACCCTCGTCGGTCCTACGGT
CGCTTCCCACCGGTACGGTA

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDIPDDYPLDEHSVRAYEDQLLD
PELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAIAVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSW
DVGKALVANMIALRRLQEHVAERAGLEPTTLTVYAANAHVYEEDLPDLP

Sequences:

>Translated_209_residues
MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDIPDDYPLDEHSVRAYEDQLLD
PELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAIAVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSW
DVGKALVANMIALRRLQEHVAERAGLEPTTLTVYAANAHVYEEDLPDLP
>Mature_209_residues
MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDIPDDYPLDEHSVRAYEDQLLD
PELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAIAVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSW
DVGKALVANMIALRRLQEHVAERAGLEPTTLTVYAANAHVYEEDLPDLP

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TYSY_METKA (Q8TVL9)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614653.1
- ProteinModelPortal:   Q8TVL9
- SMR:   Q8TVL9
- GeneID:   1477965
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1370
- NMPDR:   fig|190192.1.peg.1366
- HOGENOM:   HBG540931
- OMA:   VANMIAL
- ProtClustDB:   PRK00956
- BioCyc:   MKAN190192:MK1370-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_01686
- InterPro:   IPR000398
- InterPro:   IPR014620
- Gene3D:   G3DSA:3.30.572.10
- PIRSF:   PIRSF036752
- TIGRFAMs:   TIGR03283

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: 2.1.1.45

Molecular weight: Translated: 23781; Mature: 23781

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDI
CCCCCCEEEEECCCHHHHHHHHHCCEEECCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
PDDYPLDEHSVRAYEDQLLDPELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAI
CCCCCCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEE
AVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSWDVGKALVANMIALRRLQEHV
EEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHH
AERAGLEPTTLTVYAANAHVYEEDLPDLP
HHHCCCCCCEEEEEECCCEEECCCCCCCC
>Mature Secondary Structure
MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDI
CCCCCCEEEEECCCHHHHHHHHHCCEEECCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
PDDYPLDEHSVRAYEDQLLDPELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAI
CCCCCCCCCHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEE
AVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSWDVGKALVANMIALRRLQEHV
EEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHH
AERAGLEPTTLTVYAANAHVYEEDLPDLP
HHHCCCCCCEEEEEECCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014