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The map label for this gene is IlvB_1 [C]

Identifier: 20093886

GI number: 20093886

Start: 418732

End: 419226

Strand: Reverse

Name: IlvB_1 [C]

Synonym: MK0448

Alternate gene names: 20093886

Gene position: 419226-418732 (Counterclockwise)

Preceding gene: 20093888

Following gene: 20093885

Centisome position: 24.73

GC content: 61.62

Gene sequence:

>495_bases
TTGCGGCCGGAGGAAGCTCTCCTCGCACTTCGGAAGGTCTTGAAAGAGGAGGATCGGGTGATCGTGGACTTAGGCGATCA
CACCATCGCCGCGGCCAAGGTAGGCTTGGATCCGGACGGAGGCACTGGCGCGCTGGGCGGCTCGATGTCCGTGGCGTTGG
GTCACTGCCTCGGAACCGACGGTGGGAGGGTCTACTGTATCGTCGGCGACGGTGGCTTCTTCATGCATTTACACACTTTG
GCTACCGTGGCTCAGAACCGCGATCGCTTCGAGAACTTGACGGTGGTGGTCGTGACCGATGCGGCGTGGGGGATGACGGG
AGGCCAGGAGAACCCGGCCGTTCACACCTCGCCGGCCGATATAGCCCGTTCCATGGGTTTCGACCCCGCCGAGATCGCCG
AAAGCGTCGAAGAGGCACTGGAGGTGCTGAAGCGGTGTCGGAAGGAAGGACCGAGTCTAGTGGAGATGAGATGTCGACCG
ATTACCTTCCTATAG

Upstream 100 bases:

>100_bases
GAAGAGCATGAGCACCTCATGCCCCCAGAGGGTAGCGAAGTGGAGGTGGATCTGGACAAGGGTATAGTGTACCTGCTGGA
ATGATAGTGAGGGAACGACG

Downstream 100 bases:

>100_bases
GGTCACACGAGGTCATCCGCGCCCTTCACGACCTCGAACCCGACGTCGTGGCGTACCTGCCGGGATTTCCGCTCAACGAG
GTCGTCCGCGCACTGGAGGA

Product: acetolactate synthase large subunit

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTDGGRVYCIVGDGGFFMHLHTL
ATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPADIARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRP
ITFL

Sequences:

>Translated_164_residues
MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTDGGRVYCIVGDGGFFMHLHTL
ATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPADIARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRP
ITFL
>Mature_164_residues
MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTDGGRVYCIVGDGGFFMHLHTL
ATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPADIARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRP
ITFL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 17472; Mature: 17472

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTD
CCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
GGRVYCIVGDGGFFMHLHTLATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPAD
CCEEEEEEECCCHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCCCCCCCCCEECCHHH
IARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRPITFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTD
CCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
GGRVYCIVGDGGFFMHLHTLATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPAD
CCEEEEEEECCCHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCCCCCCCCCEECCHHH
IARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRPITFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA