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The map label for this gene is TrpA

Identifier: 20093841

GI number: 20093841

Start: 383306

End: 384133

Strand: Direct

Name: TrpA

Synonym: MK0403

Alternate gene names: 20093841

Gene position: 383306-384133 (Clockwise)

Preceding gene: 20093840

Following gene: 20093843

Centisome position: 22.61

GC content: 60.51

Gene sequence:

>828_bases
GTGTCCGGTTTGGTCCTAGAAAGGATCTTCGAGGAAGCTGAAGGCGAAGGACGTGGTGCGTTGATCGGGTACTTGACCTG
TGGACACCCAGGACTCGAAGAGACCGTCTCACTGGCTCGGGCGCTTCGTGATGGAGGTGTGGATATCCTGGAACTCGGGG
TTCCGTTCTCCGAGCCCATCGCCGACGGTCCGACGATACAGAAGGCGGTAGACGAGGCGTTACGAGCGGGCACTACACCG
TGGGACTGCTTAGAGGTCGCAGAGGAGGTGTCCGAGTTCGTCCCCGTCGTGCTCCTATGTTACTACAATACGCTCCACGC
CAACGGATTTGAGCGATATCTCTCGGCGGCGGCGGAGGCCGGTGTCTCCGGAATCATAGTGGCGGACATGCCGGTCGAGG
AATCCGACGAGGTCCACTCCGTCGCTCGAGATCTCGAGATCGACGTGATCTACCTGGTAGCCCCTTCCACGACCGATGAG
CGGCTGAAGAAGATAGGGGAAAGAGCCTCAGGGTTCGTGTACGTGATCTCCAGGTACGGCGTCACCGGAGCCCGACGGGA
TCTTTCCGAAGATACCCTGGAGCTCGTTCGTTGGGTGCGTGACCACGTCGACGTACCGGTAGCCGTGGGGTTCGGGATCT
CGGAACGTTGGCACGTGGAGGAGGTAATAGCCGCGGGCGCTGACGGGGCGATCGTTGGATCGGCGTTCATCAAGGAGATA
CACCGGTCCGAGGACATAGCGGAAGCGGAAGAGCGGGTTAGAGAGCTCGCGAAGGAGTTGGTGGAGGGTGCCCGGGACGG
GTACCGTCGAAGATCATCCTCTGAGTGA

Upstream 100 bases:

>100_bases
CCCACTCTTCATCGGTGACACCTCGTAGACCCGGTTGACACATCATCCCCGACCCATGGGAAAAGTCGCTTTACGGACCA
CCGAGTCGCCAGTCCAGGGA

Downstream 100 bases:

>100_bases
CTTACCGGCGGCTTCTTTGAGCTTCGCAGCCAGCCGTTTAGCCGCCCGGCTCAGTGCTCTCTCTGCCTCCTCCTCGAGTA
CTTCATCCGCGATCTCCAAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPIADGPTIQKAVDEALRAGTTP
WDCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEAGVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDE
RLKKIGERASGFVYVISRYGVTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEI
HRSEDIAEAEERVRELAKELVEGARDGYRRRSSSE

Sequences:

>Translated_275_residues
MSGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPIADGPTIQKAVDEALRAGTTP
WDCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEAGVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDE
RLKKIGERASGFVYVISRYGVTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEI
HRSEDIAEAEERVRELAKELVEGARDGYRRRSSSE
>Mature_274_residues
SGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPIADGPTIQKAVDEALRAGTTPW
DCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEAGVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDER
LKKIGERASGFVYVISRYGVTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEIH
RSEDIAEAEERVRELAKELVEGARDGYRRRSSSE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=266, Percent_Identity=35.3383458646617, Blast_Score=151, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=275, Percent_Identity=41.0909090909091, Blast_Score=203, Evalue=3e-53,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_METKA (Q8TYA2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613688.1
- ProteinModelPortal:   Q8TYA2
- SMR:   Q8TYA2
- GeneID:   1477706
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0403
- NMPDR:   fig|190192.1.peg.401
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- BioCyc:   MKAN190192:MK0403-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29942; Mature: 29811

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 51-51 ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPI
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
ADGPTIQKAVDEALRAGTTPWDCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDERLKKIGERASGFVYVISRYG
CCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHC
VTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEI
CCHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
HRSEDIAEAEERVRELAKELVEGARDGYRRRSSSE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPI
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
ADGPTIQKAVDEALRAGTTPWDCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDERLKKIGERASGFVYVISRYG
CCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHC
VTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEI
CCHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
HRSEDIAEAEERVRELAKELVEGARDGYRRRSSSE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014