Click here to switch to the map view.

The map label for this gene is trmE [C]

Identifier: 20093476

GI number: 20093476

Start: 30390

End: 30980

Strand: Reverse

Name: trmE [C]

Synonym: MK0036

Alternate gene names: 20093476

Gene position: 30980-30390 (Counterclockwise)

Preceding gene: 20093479

Following gene: 20093475

Centisome position: 1.83

GC content: 62.27

Gene sequence:

>591_bases
ATGAAAGAGACCTCCGTGAAAGAGGTTCCGGAGTTGGACTCGCGGGCCTTTCGTATCGCCGTGTTGGGGCCTGAAAACGC
CGGCAAGTCCACTCTGGTCAACGCGCTGATGGGTCGCGAGGTCACTGAGGTCTCCGAAGTCCCCGGTACCACGAAGACGG
TCTCCGGTTACCGTTGGACGTCCCGCGAGTTCCCCCTGTACGTGTTCGATACCGTCGGACTCGCCGACGAGCGGGGTAAG
CGAAGTAAACGAGGTGTCCGTGCCGAGGACGTCGCCGAGAAGCTCGGCAGATACGATCTGGCGCTGTTCGTGGTCGACGT
CACGCGGCATGTAGGTCCCGAAACGCTCCGTGCACTCCACGTGATCAAGTACGCCGCCGACCTCGAGACGCTGCTCGTCG
CGAACAAGATCGATCTTCTCGACCGCGGGGAACTTGAGGAACGGTTAGAACGCATCAGGGAGCGTACTGGTCACAGACCC
ATCCCCGTGTCCGCCCTTACCGGCGAGGGTATCGGGAGGCTGCTCAGGGAGATCGAGCGTCGGGTTCGTGAGAAGCGACG
AACGCTACGGGCCGCTCCGGGGTACCGGTAA

Upstream 100 bases:

>100_bases
AATACGTGCAACATAGACCGCTCAAGGTGTTGCTCCCGGTTTTGGGAGTGATCCTGGGGTTCTTGGCAGGTTTAGCCCGG
GTTGTGCGGGGGGTGAGGGG

Downstream 100 bases:

>100_bases
TACTATCAGCACTGGCCGGCGCGGATCGCGGTGAAGCCGGGGGGCGGCTACAGTTGACGGTGCTACGTTCGATCGATGAG
CTTGCCGAGCTCAACCCGTT

Product: small, Ras-like GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWTSREFPLYVFDTVGLADERGK
RSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALHVIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRP
IPVSALTGEGIGRLLREIERRVREKRRTLRAAPGYR

Sequences:

>Translated_196_residues
MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWTSREFPLYVFDTVGLADERGK
RSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALHVIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRP
IPVSALTGEGIGRLLREIERRVREKRRTLRAAPGYR
>Mature_196_residues
MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWTSREFPLYVFDTVGLADERGK
RSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALHVIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRP
IPVSALTGEGIGRLLREIERRVREKRRTLRAAPGYR

Specific function: Exhibits A Very High Intrinsic Gtpase Hydrolysis Rate. Involved In The Biosynthesis Of The Hypermodified Nucleoside 5- Methylaminomethyl-2-Thiouridine, Which Is Found In The Wobble Position Of Some Trnas. [C]

COG id: COG1100

COG function: function code R; GTPase SAR1 and related small G proteins

Gene ontology:

Cell location: Cytoplasmic; may also be associated with the inner membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=173, Percent_Identity=31.2138728323699, Blast_Score=64, Evalue=9e-11,
Organism=Homo sapiens, GI193082988, Length=173, Percent_Identity=31.2138728323699, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI2367268, Length=168, Percent_Identity=31.547619047619, Blast_Score=66, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323665, Length=165, Percent_Identity=30.3030303030303, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225 [H]

Pfam domain/function: PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 22133; Mature: 22133

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWT
CCCCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEC
SREFPLYVFDTVGLADERGKRSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALH
CCCCCEEEEECCCCCHHCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHH
VIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRPIPVSALTGEGIGRLLREIER
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHH
RVREKRRTLRAAPGYR
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWT
CCCCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEC
SREFPLYVFDTVGLADERGKRSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALH
CCCCCEEEEECCCCCHHCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHH
VIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRPIPVSALTGEGIGRLLREIER
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHH
RVREKRRTLRAAPGYR
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]