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The map label for this gene is glpF [H]

Identifier: 19746594

GI number: 19746594

Start: 1408928

End: 1409629

Strand: Reverse

Name: glpF [H]

Synonym: spyM18_1694

Alternate gene names: 19746594

Gene position: 1409629-1408928 (Counterclockwise)

Preceding gene: 19746595

Following gene: 19746593

Centisome position: 74.39

GC content: 46.01

Gene sequence:

>702_bases
ATGGATATCTTTGGTGAATTTTGGGATACAGCCTTATTGGTTTTACTAGGAAATGGTGTGGTAGCAGGGGTTGTCTTGCC
CAAAACGAAAACCCATGCTTCTGGCTGGATCGTGATTGCGACTGGCTGGGGAATTGCAGTTGCTGTGGCAGTCTTTATCA
GTGGCAAAGTTGCCCCTGCCCACCTCAATCCAGCTGTTAGCCTTGCTTTTGCCATGAGTGGAACCATTGCTTGGTCAACA
GCCATTGCCTATAGCCTTGCCCAACTATTGGGAGCTATGGTTGGTTCAACTCTGGTATTCCTGCAGTTCAGGCCACATTA
TCTGGCTGCTGAGAGTCAGGCTGATATTTTAGGGACATTTGCGACAGGTCCTGCTATTCGAGATACTAGCTCAAACCTAT
TGAGTGAAATCTTTGGGACCTTTGTCTTGATGCTTGGTATTTTGGCATTTGGCTTATATGATATGCCAGCAGGACTAGGA
ACCCTCTGTGTAGGTACCTTGGTTATTGGGATTGGATTATCCTTAGGAGGAACGACAGGTTACGCCATTAACCCAGCGCG
TGATTTAGGACCTCGTCTAGTTCATGCTATTCTACCACTCAACAACAAGGGAGATTCTGATTGGTCTTATGCTTGGATAC
CTGTTGTAGGACCAATTATTGGAGTGGCTTTAGCAGTTTTGCTTTTCCAAGTCATGTCCTAA

Upstream 100 bases:

>100_bases
GAATGGTCTGATCAGGAAAGAGTGGCACAAGAAGACGATCTTCGTCGCGTGATTGCAGACAATGATTTGAGTGCCCTAAA
AGGCCAGCAGGAGGGTTAAT

Downstream 100 bases:

>100_bases
CCATCACTAAAGGGGTTGGCTATCATAGGCCAGCTCCTTTGCTTTTTCTTTATAGGATTAGGTAAAACGCTTTCATATCT
TAAAAACAACTGCTATAATA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDIFGEFWDTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGVALAVLLFQVMS

Sequences:

>Translated_233_residues
MDIFGEFWDTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGVALAVLLFQVMS
>Mature_233_residues
MDIFGEFWDTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWST
AIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGVALAVLLFQVMS

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=245, Percent_Identity=35.1020408163265, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=35.9504132231405, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=32.520325203252, Blast_Score=103, Evalue=9e-23,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=31.8367346938775, Blast_Score=94, Evalue=7e-20,
Organism=Homo sapiens, GI4502181, Length=199, Percent_Identity=28.1407035175879, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4755125, Length=199, Percent_Identity=28.1407035175879, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI37694062, Length=235, Percent_Identity=28.5106382978723, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=32.520325203252, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71994009, Length=226, Percent_Identity=32.7433628318584, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=32.0833333333333, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=32.0833333333333, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=244, Percent_Identity=33.1967213114754, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71992966, Length=246, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17558372, Length=235, Percent_Identity=30.6382978723404, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32564052, Length=232, Percent_Identity=30.1724137931034, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17533613, Length=232, Percent_Identity=30.1724137931034, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=219, Percent_Identity=29.2237442922374, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322985, Length=212, Percent_Identity=29.2452830188679, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24762342, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24112; Mature: 24112

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFGEFWDTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAVFISGKVAPA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCC
HLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTF
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCHHEEEC
ATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLGTLCVGTLVIGIGLSLGGTTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
YAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGVALAVLLFQVMS
CEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDIFGEFWDTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWGIAVAVAVFISGKVAPA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCC
HLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQFRPHYLAAESQADILGTF
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCHHEEEC
ATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLGTLCVGTLVIGIGLSLGGTTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
YAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGPIIGVALAVLLFQVMS
CEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]