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The map label for this gene is lysK [H]

Identifier: 18977557

GI number: 18977557

Start: 1130502

End: 1131554

Strand: Direct

Name: lysK [H]

Synonym: PF1185

Alternate gene names: 18977557

Gene position: 1130502-1131554 (Clockwise)

Preceding gene: 18977552

Following gene: 18977560

Centisome position: 59.24

GC content: 42.36

Gene sequence:

>1053_bases
ATGAAAACCGAGAGAGCAAAAGAGATATTAGTTCAGCTTTTGAGAATTCCATCTCCCTCTGGGAGAGAGGACAGAATAGC
ACTTTACATAATGGATTTCCTGCACAGGCTTGACTATGACGTTCATATTGAGAGTGATGGAGAGATAATAGATCTAGTAG
TTAATCCAGAGGCCGAACTATTCTATGAAGTTCACATGGACACAATAGAGCCAAGGGCAGAACCCTTCGTAAGGGGGAAC
ATAGTTTACGGAACGGGAGCTAGCGACATAAAGGGAGGCATTGCAGCGATTCTGCTAATGCTTGAGCAACTAAGAAAAGA
GAACAAAGACTTGAATGTTGGGATAGTTTTTGTAAGCGATGAAGAGAAAGGTGGAAGGGGTAGTGCCCTCTTCATGGAGA
GGTACAGGCCCAAGATGGCCATAGTTCTTGAGCCAACTGATTTAGAAGTGCATATAGCACACGCTGGAAATATTGAAGCC
TATTTCGAAGTTGATGGAAAGGAAGCGCATGGAGCATGTCCGGAGAGTGGAATAAATGCAATAGAGCAGACCTTCGAAAT
GTTGCAAAAGCTGAAGGAACTCGAACCTTTCAAAGTAAAAGGGAAGTACTTTGACCCACACATAGGAATACAGGAGCTAA
TATGTGAAAACCCATTCTACTTAATTCCAGGGCTTTGTAAGGGACGATTAGAGGCAAGGTTACTACCAGAGCAGGAAGTA
GAGGATGTACTAGACTTGATTGATCCAATCTTAGATGAATATACCATTAGGCATGAATACACTGAAATTTGGGACGGGTA
TGAGCTAAGTGAGGATGAGGAAATTGTTCAGATTGCAAAGAAAGCAATGGAGGCAACGGGCCTCAACGAATTTGGTGGAA
TGAGGAGCTGGACAGATGCAATAAACTTCATGTACAACGGAACAAAGACGATAGTATTTGGCCCAGGGAACCTTGACATC
TCACACACAAAGAACGAAAGAATTGATGTGAGGGATGTAGTAAAGGCAAGTGAATTCCTAATGTATGTAAATGAAATATA
TGGAAAGAGCTAG

Upstream 100 bases:

>100_bases
TGATGGTGGGCACTCGCCGATTGGTGAGGTAAGGGGCGAGGAGACAGAGGCCCAAATATATTTATAATCTCCTTTTCCTT
TAGATAATTAGGTGAGCTTA

Downstream 100 bases:

>100_bases
AACTTCAAAACCCTGGCGAGGACTATCAAGGGATCCCAAACCGGGGCAAAGGGTGGTGCATAGGCTAGGTCTGTGAAGAA
GACATCCTTAGTTGTGAAGC

Product: acetylornithine deacetylase

Products: acetate; L-ornithine

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKTERAKEILVQLLRIPSPSGREDRIALYIMDFLHRLDYDVHIESDGEIIDLVVNPEAELFYEVHMDTIEPRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEQLRKENKDLNVGIVFVSDEEKGGRGSALFMERYRPKMAIVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIEQTFEMLQKLKELEPFKVKGKYFDPHIGIQELICENPFYLIPGLCKGRLEARLLPEQEV
EDVLDLIDPILDEYTIRHEYTEIWDGYELSEDEEIVQIAKKAMEATGLNEFGGMRSWTDAINFMYNGTKTIVFGPGNLDI
SHTKNERIDVRDVVKASEFLMYVNEIYGKS

Sequences:

>Translated_350_residues
MKTERAKEILVQLLRIPSPSGREDRIALYIMDFLHRLDYDVHIESDGEIIDLVVNPEAELFYEVHMDTIEPRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEQLRKENKDLNVGIVFVSDEEKGGRGSALFMERYRPKMAIVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIEQTFEMLQKLKELEPFKVKGKYFDPHIGIQELICENPFYLIPGLCKGRLEARLLPEQEV
EDVLDLIDPILDEYTIRHEYTEIWDGYELSEDEEIVQIAKKAMEATGLNEFGGMRSWTDAINFMYNGTKTIVFGPGNLDI
SHTKNERIDVRDVVKASEFLMYVNEIYGKS
>Mature_350_residues
MKTERAKEILVQLLRIPSPSGREDRIALYIMDFLHRLDYDVHIESDGEIIDLVVNPEAELFYEVHMDTIEPRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEQLRKENKDLNVGIVFVSDEEKGGRGSALFMERYRPKMAIVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIEQTFEMLQKLKELEPFKVKGKYFDPHIGIQELICENPFYLIPGLCKGRLEARLLPEQEV
EDVLDLIDPILDEYTIRHEYTEIWDGYELSEDEEIVQIAKKAMEATGLNEFGGMRSWTDAINFMYNGTKTIVFGPGNLDI
SHTKNERIDVRDVVKASEFLMYVNEIYGKS

Specific function: Displays A Broad Specificity And Can Also Deacylate Substrates Such As Acetylarginine, Acetylhistidine Or Acetylglutamate Semialdehyde. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. LysK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=281, Percent_Identity=24.9110320284698, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789236, Length=362, Percent_Identity=24.585635359116, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010175
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.16

Molecular weight: Translated: 39805; Mature: 39805

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTERAKEILVQLLRIPSPSGREDRIALYIMDFLHRLDYDVHIESDGEIIDLVVNPEAEL
CCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCEEEEEECCCCEE
FYEVHMDTIEPRAEPFVRGNIVYGTGASDIKGGIAAILLMLEQLRKENKDLNVGIVFVSD
EEEEECCCCCCCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
EEKGGRGSALFMERYRPKMAIVLEPTDLEVHIAHAGNIEAYFEVDGKEAHGACPESGINA
CCCCCCCCCHHHHHCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHH
IEQTFEMLQKLKELEPFKVKGKYFDPHIGIQELICENPFYLIPGLCKGRLEARLLPEQEV
HHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCEEECCHHCCCHHHEECCHHHH
EDVLDLIDPILDEYTIRHEYTEIWDGYELSEDEEIVQIAKKAMEATGLNEFGGMRSWTDA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHH
INFMYNGTKTIVFGPGNLDISHTKNERIDVRDVVKASEFLMYVNEIYGKS
HHHEECCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTERAKEILVQLLRIPSPSGREDRIALYIMDFLHRLDYDVHIESDGEIIDLVVNPEAEL
CCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCEEEEEECCCCEE
FYEVHMDTIEPRAEPFVRGNIVYGTGASDIKGGIAAILLMLEQLRKENKDLNVGIVFVSD
EEEEECCCCCCCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
EEKGGRGSALFMERYRPKMAIVLEPTDLEVHIAHAGNIEAYFEVDGKEAHGACPESGINA
CCCCCCCCCHHHHHCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHH
IEQTFEMLQKLKELEPFKVKGKYFDPHIGIQELICENPFYLIPGLCKGRLEARLLPEQEV
HHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCEEECCHHCCCHHHEECCHHHH
EDVLDLIDPILDEYTIRHEYTEIWDGYELSEDEEIVQIAKKAMEATGLNEFGGMRSWTDA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHH
INFMYNGTKTIVFGPGNLDISHTKNERIDVRDVVKASEFLMYVNEIYGKS
HHHEECCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Glutathione. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N2-acetyl-L-ornithine; H2O

Specific reaction: N2-acetyl-L-ornithine + H2O = acetate + L-ornithine

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]