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The map label for this gene is pyk [H]

Identifier: 18977560

GI number: 18977560

Start: 1134061

End: 1135494

Strand: Direct

Name: pyk [H]

Synonym: PF1188

Alternate gene names: 18977560

Gene position: 1134061-1135494 (Clockwise)

Preceding gene: 18977557

Following gene: 18977561

Centisome position: 59.43

GC content: 40.73

Gene sequence:

>1434_bases
GTGAGAAGGGTGAAGCTCCCATCTCACAAGACAAAAATTGTAGCTACAATAGGGCCAGCGACTAATTCGAGAAAAATGAT
AAAACAACTCATTAAGGCGGGGATGAATGTTGCAAGAATAAACTTCTCCCATGGAAGTTTTGAAGAGCATGCAAGAGTCA
TAGAAATAATAAGAGAGGAGGCACAAAAATTAGACAGGAGAGTTGCAATACTTGCAGATCTACCTGGATTGAAAATAAGA
GTCGGTGAGATAAAAGGGGGTTACGTAGAGCTAAAGAGAGGAGAAAAGGTAATATTAACAACAAAGGATGTTGAGGGTGA
TGAAACTACGATACCAGTGGATTATAAAGGCTTTCCAAATTTGGTATCGAAGGGAGACATCATATACCTTAATGACGGAT
ACATAGTCTTAAAGGTGGAGAACGTTAGGGAGAACGAAGTTGAAGCAGTTGTTCTTTCAGGAGGAAAGCTCTTCTCGAGA
AAGGGCGTCAATATACCCAAGGCCTACCTCCCAGTCGAAGCCATAACTCCAAAAGACTTTGAAATTATGAAATTTGCAAT
TGAGCATGGGGTGGATGCAATAGGATTGAGCTTCGTTGGGAGCGTTTATGATGTTCTAAAGGCCAAGAGTTTCCTTGAGA
AAAATAATGCCGAGGATGTATTTGTAATTGCAAAGATCGAAAGACCTGACGCCGTGAGGAATTTTGACGAGATTCTTAAT
GCCGCTGATGGAATTATGATTGCTAGAGGAGATTTAGGGGTAGAGATGCCGATAGAGCAGCTACCAATTCTTCAGAAGAA
ATTGATCAGGAAAGCCAATATGGAAGGGAAACCAGTGATTACGGCAACACAAATGCTTGTGTCAATGACAACGGAAAAAG
TGCCAACAAGGGCCGAAGTTACGGACGTTGCAAACGCAATTCTTGATGGAACGGATGCAGTTATGCTATCAGAAGAAACG
GCCATAGGAAAATTCCCCATTGAGACCGTAGAGATGATGGGAAAGATAGCTAAGGTCACTGAAGAATATAGGGAAAGTTT
TGGGTTAAGCAGAATAAGGGAGTTTATGGAGATCAAAAAGGGAACAATAAAAGAGGCTATCACTAGAAGTATAATTGACG
CAATATGCACAATAGACATTAAGTTTATACTAACACCCACCAGAACGGGAAGAACTGCTAGGCTAATCTCAAGATTTAAG
CCCAAGCAGTGGATTTTAGCCTTCTCAACAAATGAAAGGGTGTGCAACAATCTCATGTTCAGCTATGGAGTTTATCCCTT
CTGCCTAGAAGAGGGATTCGATGAGAACGATATAGTTAGGTTGATAAAAGGACTGGGCCTTGTGGAAAGCGATGATATGG
TTTTAATGACGGAAGGAAAGCCAATTGAAAAAACAGTTGGAACTAACTCAATTAAGATATTCCAAATAGCTTGA

Upstream 100 bases:

>100_bases
CACATCAAAACTCTTAGTTCCTGGATCAACTCCAACGGCCCTCATGGTTGAGTCTTCTGGCGAATATTTTTTATTGTTTT
CGAAGAAAAATTAGGGCAAG

Downstream 100 bases:

>100_bases
GGTGAGAAAAATGAAGGTTTATTACCCAGGGAGAGAGTGGCCTGAGGAATACAAAAGAGTTTTGGAAAAGATGAAAGAAA
TAATTGATCCAGTTACTGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRVKLPSHKTKIVATIGPATNSRKMIKQLIKAGMNVARINFSHGSFEEHARVIEIIREEAQKLDRRVAILADLPGLKIR
VGEIKGGYVELKRGEKVILTTKDVEGDETTIPVDYKGFPNLVSKGDIIYLNDGYIVLKVENVRENEVEAVVLSGGKLFSR
KGVNIPKAYLPVEAITPKDFEIMKFAIEHGVDAIGLSFVGSVYDVLKAKSFLEKNNAEDVFVIAKIERPDAVRNFDEILN
AADGIMIARGDLGVEMPIEQLPILQKKLIRKANMEGKPVITATQMLVSMTTEKVPTRAEVTDVANAILDGTDAVMLSEET
AIGKFPIETVEMMGKIAKVTEEYRESFGLSRIREFMEIKKGTIKEAITRSIIDAICTIDIKFILTPTRTGRTARLISRFK
PKQWILAFSTNERVCNNLMFSYGVYPFCLEEGFDENDIVRLIKGLGLVESDDMVLMTEGKPIEKTVGTNSIKIFQIA

Sequences:

>Translated_477_residues
MRRVKLPSHKTKIVATIGPATNSRKMIKQLIKAGMNVARINFSHGSFEEHARVIEIIREEAQKLDRRVAILADLPGLKIR
VGEIKGGYVELKRGEKVILTTKDVEGDETTIPVDYKGFPNLVSKGDIIYLNDGYIVLKVENVRENEVEAVVLSGGKLFSR
KGVNIPKAYLPVEAITPKDFEIMKFAIEHGVDAIGLSFVGSVYDVLKAKSFLEKNNAEDVFVIAKIERPDAVRNFDEILN
AADGIMIARGDLGVEMPIEQLPILQKKLIRKANMEGKPVITATQMLVSMTTEKVPTRAEVTDVANAILDGTDAVMLSEET
AIGKFPIETVEMMGKIAKVTEEYRESFGLSRIREFMEIKKGTIKEAITRSIIDAICTIDIKFILTPTRTGRTARLISRFK
PKQWILAFSTNERVCNNLMFSYGVYPFCLEEGFDENDIVRLIKGLGLVESDDMVLMTEGKPIEKTVGTNSIKIFQIA
>Mature_477_residues
MRRVKLPSHKTKIVATIGPATNSRKMIKQLIKAGMNVARINFSHGSFEEHARVIEIIREEAQKLDRRVAILADLPGLKIR
VGEIKGGYVELKRGEKVILTTKDVEGDETTIPVDYKGFPNLVSKGDIIYLNDGYIVLKVENVRENEVEAVVLSGGKLFSR
KGVNIPKAYLPVEAITPKDFEIMKFAIEHGVDAIGLSFVGSVYDVLKAKSFLEKNNAEDVFVIAKIERPDAVRNFDEILN
AADGIMIARGDLGVEMPIEQLPILQKKLIRKANMEGKPVITATQMLVSMTTEKVPTRAEVTDVANAILDGTDAVMLSEET
AIGKFPIETVEMMGKIAKVTEEYRESFGLSRIREFMEIKKGTIKEAITRSIIDAICTIDIKFILTPTRTGRTARLISRFK
PKQWILAFSTNERVCNNLMFSYGVYPFCLEEGFDENDIVRLIKGLGLVESDDMVLMTEGKPIEKTVGTNSIKIFQIA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=446, Percent_Identity=39.9103139013453, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI32967597, Length=446, Percent_Identity=39.9103139013453, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI33286418, Length=479, Percent_Identity=37.160751565762, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI33286422, Length=479, Percent_Identity=36.9519832985386, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286420, Length=479, Percent_Identity=36.9519832985386, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=40.893470790378, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=40.893470790378, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=37.0833333333333, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=37.0833333333333, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=37.037037037037, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=40.6779661016949, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=41.0901467505241, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=469, Percent_Identity=39.4456289978678, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=38.7295081967213, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=38.7295081967213, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=38.7295081967213, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=38.7295081967213, Blast_Score=273, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=433, Percent_Identity=38.3371824480369, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324923, Length=466, Percent_Identity=36.0515021459227, Blast_Score=264, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=39.0776699029126, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=35.7723577235772, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=35.7723577235772, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24581235, Length=473, Percent_Identity=32.76955602537, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24646914, Length=264, Percent_Identity=34.8484848484849, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53170; Mature: 53170

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVKLPSHKTKIVATIGPATNSRKMIKQLIKAGMNVARINFSHGSFEEHARVIEIIREE
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
AQKLDRRVAILADLPGLKIRVGEIKGGYVELKRGEKVILTTKDVEGDETTIPVDYKGFPN
HHHHHHHEEEEECCCCCEEEEEECCCCEEEEECCCEEEEEECCCCCCCCEEEECCCCCCC
LVSKGDIIYLNDGYIVLKVENVRENEVEAVVLSGGKLFSRKGVNIPKAYLPVEAITPKDF
HHCCCCEEEEECCEEEEEECCCCCCCCCEEEEECCCEEECCCCCCCHHHCCEECCCCCCH
EIMKFAIEHGVDAIGLSFVGSVYDVLKAKSFLEKNNAEDVFVIAKIERPDAVRNFDEILN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
AADGIMIARGDLGVEMPIEQLPILQKKLIRKANMEGKPVITATQMLVSMTTEKVPTRAEV
CCCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
TDVANAILDGTDAVMLSEETAIGKFPIETVEMMGKIAKVTEEYRESFGLSRIREFMEIKK
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GTIKEAITRSIIDAICTIDIKFILTPTRTGRTARLISRFKPKQWILAFSTNERVCNNLMF
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
SYGVYPFCLEEGFDENDIVRLIKGLGLVESDDMVLMTEGKPIEKTVGTNSIKIFQIA
HHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEEEC
>Mature Secondary Structure
MRRVKLPSHKTKIVATIGPATNSRKMIKQLIKAGMNVARINFSHGSFEEHARVIEIIREE
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
AQKLDRRVAILADLPGLKIRVGEIKGGYVELKRGEKVILTTKDVEGDETTIPVDYKGFPN
HHHHHHHEEEEECCCCCEEEEEECCCCEEEEECCCEEEEEECCCCCCCCEEEECCCCCCC
LVSKGDIIYLNDGYIVLKVENVRENEVEAVVLSGGKLFSRKGVNIPKAYLPVEAITPKDF
HHCCCCEEEEECCEEEEEECCCCCCCCCEEEEECCCEEECCCCCCCHHHCCEECCCCCCH
EIMKFAIEHGVDAIGLSFVGSVYDVLKAKSFLEKNNAEDVFVIAKIERPDAVRNFDEILN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
AADGIMIARGDLGVEMPIEQLPILQKKLIRKANMEGKPVITATQMLVSMTTEKVPTRAEV
CCCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
TDVANAILDGTDAVMLSEETAIGKFPIETVEMMGKIAKVTEEYRESFGLSRIREFMEIKK
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GTIKEAITRSIIDAICTIDIKFILTPTRTGRTARLISRFKPKQWILAFSTNERVCNNLMF
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
SYGVYPFCLEEGFDENDIVRLIKGLGLVESDDMVLMTEGKPIEKTVGTNSIKIFQIA
HHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]