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The map label for this gene is pstS [H]

Identifier: 18977375

GI number: 18977375

Start: 960123

End: 961250

Strand: Direct

Name: pstS [H]

Synonym: PF1003

Alternate gene names: 18977375

Gene position: 960123-961250 (Clockwise)

Preceding gene: 18977374

Following gene: 18977376

Centisome position: 50.31

GC content: 42.73

Gene sequence:

>1128_bases
GTGAAGAGGCTAGCAACAATATTTATTATTTTTGCCTTGGGGCTTTCCTTAGTGGCAAGCGGTTGTATATCCGAGGGAAA
TGCATCCAATGTCCCTCAAGCAACTTCTAAAACTGAGATAATAACGATTCGCACAACAGGTGCAACTTTTCCCCAATATC
AGCTCCAAAAGTGGATTAGCATATATACAAAAAAGAACCCAAACGTTAAGATCGAGTATGAAGGTGGTGGAAGTGGGCAT
GGTCAAGAAGCATTTCTAAAGGGCCTTACAGATATAGGAAGAACAGACCCCCCAGTTAAAGAATCCACGTGGAAAAAGTT
CCTTGAAACTGGTGATCAGCCCCTTCAGTTTCCTATAGTTGTCGGTGCTGTAGTCGTCGTTCACAATATTCCAAATGTTG
ATGAGCTAAAGCTCGATGGAGAGACCTTAGCGAAAATCTTTATGGGCCAAATAGAGTATTGGGATGATCCAGCAATAAAG
TCCCTTAATCCTAATGTAAACCTTCCTCACGAAAAAATAATAGTAATTCATAGGAGTGATTCGAGTGGAACCACTGCAAT
TTTTACCACTTACTTGAGCTTAGTCAGTGAAGAATGGGCCACAAAAGTAGGAGCCGGAAAGACTGTGGATTGGCCAGTTG
ACAAAGTTGGAAGGGGAATTGGTGCAAAAGGCAACCCAGGAGTTGTTCAGGCCTTAAAGAACACCCCATATAGTATAGCA
TACACTGAGCTATCTTTTGCAATAGAGGAAAATCTCAAGATAGTAGCACTCAAAAACAAAGCCGGAAACTTCGTCAAACC
TACCGAGGAGACAATAAAAGCTGCAGTAGCCGCAGTTAAGGCCTTTATACCCGATCCCGCTGAGGGGTATAAAGAGGATA
TTAGGCAACTTCTCAATGCTCCCGGAGAAAATTCATACCCGATAGTTGCTTTTACACACTTTCTCGTCTGGCAGAATAAG
GGAGGAAAGCACTACAGTAAAGAGAAGGCTCAAGCGATTAAGGACTTTCTTAGATGGGTTTTAACAGAGGGACAGAAACC
AGAGAATATTGCTCCAGGCTACGTAGGTCTCCCACCTGAAGTTGCCGAAATAGGTCTTAAGGCAGTTGAGATGATAGAAA
CAGGTTAA

Upstream 100 bases:

>100_bases
AAATAGAGAATATATATCTATATAGATTCTTTGTTCAGATATGTTTTATTTCGACAATGCTTATATTTTCTGAATTTTCT
CTATTTCTTGGTGATGTATC

Downstream 100 bases:

>100_bases
AGGAGGTGAAAAAATGAAGAAAATAACTATTAGTAGTTTGCTTCTACTTTTACTTATTTCTACCAATTTGAATCTCGCAT
ACGATTCCCAAGAGAGCGGT

Product: phosphate-binding periplasmic protein precursor

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKRLATIFIIFALGLSLVASGCISEGNASNVPQATSKTEIITIRTTGATFPQYQLQKWISIYTKKNPNVKIEYEGGGSGH
GQEAFLKGLTDIGRTDPPVKESTWKKFLETGDQPLQFPIVVGAVVVVHNIPNVDELKLDGETLAKIFMGQIEYWDDPAIK
SLNPNVNLPHEKIIVIHRSDSSGTTAIFTTYLSLVSEEWATKVGAGKTVDWPVDKVGRGIGAKGNPGVVQALKNTPYSIA
YTELSFAIEENLKIVALKNKAGNFVKPTEETIKAAVAAVKAFIPDPAEGYKEDIRQLLNAPGENSYPIVAFTHFLVWQNK
GGKHYSKEKAQAIKDFLRWVLTEGQKPENIAPGYVGLPPEVAEIGLKAVEMIETG

Sequences:

>Translated_375_residues
MKRLATIFIIFALGLSLVASGCISEGNASNVPQATSKTEIITIRTTGATFPQYQLQKWISIYTKKNPNVKIEYEGGGSGH
GQEAFLKGLTDIGRTDPPVKESTWKKFLETGDQPLQFPIVVGAVVVVHNIPNVDELKLDGETLAKIFMGQIEYWDDPAIK
SLNPNVNLPHEKIIVIHRSDSSGTTAIFTTYLSLVSEEWATKVGAGKTVDWPVDKVGRGIGAKGNPGVVQALKNTPYSIA
YTELSFAIEENLKIVALKNKAGNFVKPTEETIKAAVAAVKAFIPDPAEGYKEDIRQLLNAPGENSYPIVAFTHFLVWQNK
GGKHYSKEKAQAIKDFLRWVLTEGQKPENIAPGYVGLPPEVAEIGLKAVEMIETG
>Mature_375_residues
MKRLATIFIIFALGLSLVASGCISEGNASNVPQATSKTEIITIRTTGATFPQYQLQKWISIYTKKNPNVKIEYEGGGSGH
GQEAFLKGLTDIGRTDPPVKESTWKKFLETGDQPLQFPIVVGAVVVVHNIPNVDELKLDGETLAKIFMGQIEYWDDPAIK
SLNPNVNLPHEKIIVIHRSDSSGTTAIFTTYLSLVSEEWATKVGAGKTVDWPVDKVGRGIGAKGNPGVVQALKNTPYSIA
YTELSFAIEENLKIVALKNKAGNFVKPTEETIKAAVAAVKAFIPDPAEGYKEDIRQLLNAPGENSYPIVAFTHFLVWQNK
GGKHYSKEKAQAIKDFLRWVLTEGQKPENIAPGYVGLPPEVAEIGLKAVEMIETG

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=324, Percent_Identity=35.8024691358025, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40888; Mature: 40888

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLATIFIIFALGLSLVASGCISEGNASNVPQATSKTEIITIRTTGATFPQYQLQKWIS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
IYTKKNPNVKIEYEGGGSGHGQEAFLKGLTDIGRTDPPVKESTWKKFLETGDQPLQFPIV
HHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEHHHH
VGAVVVVHNIPNVDELKLDGETLAKIFMGQIEYWDDPAIKSLNPNVNLPHEKIIVIHRSD
HHHHHHHHCCCCCHHEEECHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEEECC
SSGTTAIFTTYLSLVSEEWATKVGAGKTVDWPVDKVGRGIGAKGNPGVVQALKNTPYSIA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCEEE
YTELSFAIEENLKIVALKNKAGNFVKPTEETIKAAVAAVKAFIPDPAEGYKEDIRQLLNA
EHHHHEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PGENSYPIVAFTHFLVWQNKGGKHYSKEKAQAIKDFLRWVLTEGQKPENIAPGYVGLPPE
CCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VAEIGLKAVEMIETG
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLATIFIIFALGLSLVASGCISEGNASNVPQATSKTEIITIRTTGATFPQYQLQKWIS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
IYTKKNPNVKIEYEGGGSGHGQEAFLKGLTDIGRTDPPVKESTWKKFLETGDQPLQFPIV
HHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEHHHH
VGAVVVVHNIPNVDELKLDGETLAKIFMGQIEYWDDPAIKSLNPNVNLPHEKIIVIHRSD
HHHHHHHHCCCCCHHEEECHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEEECC
SSGTTAIFTTYLSLVSEEWATKVGAGKTVDWPVDKVGRGIGAKGNPGVVQALKNTPYSIA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCEEE
YTELSFAIEENLKIVALKNKAGNFVKPTEETIKAAVAAVKAFIPDPAEGYKEDIRQLLNA
EHHHHEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PGENSYPIVAFTHFLVWQNKGGKHYSKEKAQAIKDFLRWVLTEGQKPENIAPGYVGLPPE
CCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VAEIGLKAVEMIETG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]