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The map label for this gene is phoA [H]

Identifier: 18977376

GI number: 18977376

Start: 961264

End: 962748

Strand: Direct

Name: phoA [H]

Synonym: PF1004

Alternate gene names: 18977376

Gene position: 961264-962748 (Clockwise)

Preceding gene: 18977375

Following gene: 18977377

Centisome position: 50.37

GC content: 39.8

Gene sequence:

>1485_bases
ATGAAGAAAATAACTATTAGTAGTTTGCTTCTACTTTTACTTATTTCTACCAATTTGAATCTCGCATACGATTCCCAAGA
GAGCGGTATTAAAAATATAATAATCCTCATTGGAGACGGCATGGGAATGAGTCATGTCCAGATTACAAAGCTTGTTTATG
GTCATCTAAACATGGAAGAGTTCCCAATTATTGGATTCGAACTTACTGAGTCATTAAGTGGGGAAGTTACGGACTCCGCT
GCAGCAGGAACTGCAATAGCAACTGGAGTCAAAACATATAATCGAATGATTTCAGTTACTAACATAACTGGAAAAGTTAC
AAATCTAACTACCTTGCTTGAAATAGCCCAGGTACTTGGAAAATCAACTGGACTTGTGACTACTACTAGAATTACACACG
CAACCCCTGCAGTATTTGCTTCCCACGTTCCTGACAGAGATATGGAAGAGGAAATAGCGAGACAGCTCATAGCTCACCGG
GTCAACGTCCTATTAGGTGGAGGGAGAAAGAAATTTGACGAGAATACCCTAAAAATGGCAAAAGAACAGGGATATAATAT
AGTCTTCACGAAAGAAGAGCTCGAGAAAGCAGAGGGTGAGTTTATTCTAGGGCTTTTTGCAGATAGCCACATTCCTTACG
TATTGGACAGAAAACCAGAAGATGTTGGACTTTTGGAAATGACTAAAAAAGCAATTTCAATACTAGAGAAAAATCCAAAT
GGGTTCTTTCTCATGATTGAAGGGGGCAGAATTGATCATGCAGCTCATGAGAATGATATAGCATCAGTTGTTGCAGAGAC
TAAGGAGTTTGATGACGTTGTTGGATATGTTCTTGAGTATGCAAAAAAGAGGGGAGATACACTAGTAATAGTGCTGGCTG
ACCATGAGACAGGGGGGCTTGGATTAGGTCTAACATATGGAGATGCAATTAATGAAGATGTCATCAGGAACATAAACGCT
AGTGTGTCGAAAATTGCTAGTGAAATAAGGGCAACGAATGACATAAAGAGAGTTATCAAAAAATATACTGGATTCGAGCT
AACAGAGGACGAAATTAATTACATTGAGGAAGCTATAAACTTAGCAGACGAATATGCGCTTCAAAATGCAATAGCTGATA
TTATAAACAAACGCGTTGGTGTAGGTTTTGTATCCCACAAACATACAGGAGCTCCTGTTTCACTTCTAGCCTACGGCCCA
GGTGCAGAGAATTTTGCAGGCTTTTTACACCATGTAGATACGGCAAAGCTAATTGCCAAGCTAATGCTCTTTGGGAAGAA
AGATATTCCCGTTACCATCTTGGGAATAAGTGGAGTTAAAGGAGATATAACCGGAGACTTCAAAGTGGATGAGCAAGATG
CATATGTGACCTTAATGATGTTGCTTGGGGAAAGGGTAGATACTGAACTTGAAAGGAAAGTCGACATGAATAATAACGGC
ATAATCGAGTTGGGAGACGTGCTCCTGATTCTACAAGAGTCCTAA

Upstream 100 bases:

>100_bases
GAGAATATTGCTCCAGGCTACGTAGGTCTCCCACCTGAAGTTGCCGAAATAGGTCTTAAGGCAGTTGAGATGATAGAAAC
AGGTTAAAGGAGGTGAAAAA

Downstream 100 bases:

>100_bases
CTTCTCATTATTTAATTTTTTTGGTGATGAATCATGAAAATAGGTGTTAATTCGTATATAGTGAGGGAAATGACTGGAAG
AGGATTTTCGTTAGATGAAC

Product: alkaline phosphatase IV precursor

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKKITISSLLLLLLISTNLNLAYDSQESGIKNIIILIGDGMGMSHVQITKLVYGHLNMEEFPIIGFELTESLSGEVTDSA
AAGTAIATGVKTYNRMISVTNITGKVTNLTTLLEIAQVLGKSTGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIAHR
VNVLLGGGRKKFDENTLKMAKEQGYNIVFTKEELEKAEGEFILGLFADSHIPYVLDRKPEDVGLLEMTKKAISILEKNPN
GFFLMIEGGRIDHAAHENDIASVVAETKEFDDVVGYVLEYAKKRGDTLVIVLADHETGGLGLGLTYGDAINEDVIRNINA
SVSKIASEIRATNDIKRVIKKYTGFELTEDEINYIEEAINLADEYALQNAIADIINKRVGVGFVSHKHTGAPVSLLAYGP
GAENFAGFLHHVDTAKLIAKLMLFGKKDIPVTILGISGVKGDITGDFKVDEQDAYVTLMMLLGERVDTELERKVDMNNNG
IIELGDVLLILQES

Sequences:

>Translated_494_residues
MKKITISSLLLLLLISTNLNLAYDSQESGIKNIIILIGDGMGMSHVQITKLVYGHLNMEEFPIIGFELTESLSGEVTDSA
AAGTAIATGVKTYNRMISVTNITGKVTNLTTLLEIAQVLGKSTGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIAHR
VNVLLGGGRKKFDENTLKMAKEQGYNIVFTKEELEKAEGEFILGLFADSHIPYVLDRKPEDVGLLEMTKKAISILEKNPN
GFFLMIEGGRIDHAAHENDIASVVAETKEFDDVVGYVLEYAKKRGDTLVIVLADHETGGLGLGLTYGDAINEDVIRNINA
SVSKIASEIRATNDIKRVIKKYTGFELTEDEINYIEEAINLADEYALQNAIADIINKRVGVGFVSHKHTGAPVSLLAYGP
GAENFAGFLHHVDTAKLIAKLMLFGKKDIPVTILGISGVKGDITGDFKVDEQDAYVTLMMLLGERVDTELERKVDMNNNG
IIELGDVLLILQES
>Mature_494_residues
MKKITISSLLLLLLISTNLNLAYDSQESGIKNIIILIGDGMGMSHVQITKLVYGHLNMEEFPIIGFELTESLSGEVTDSA
AAGTAIATGVKTYNRMISVTNITGKVTNLTTLLEIAQVLGKSTGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIAHR
VNVLLGGGRKKFDENTLKMAKEQGYNIVFTKEELEKAEGEFILGLFADSHIPYVLDRKPEDVGLLEMTKKAISILEKNPN
GFFLMIEGGRIDHAAHENDIASVVAETKEFDDVVGYVLEYAKKRGDTLVIVLADHETGGLGLGLTYGDAINEDVIRNINA
SVSKIASEIRATNDIKRVIKKYTGFELTEDEINYIEEAINLADEYALQNAIADIINKRVGVGFVSHKHTGAPVSLLAYGP
GAENFAGFLHHVDTAKLIAKLMLFGKKDIPVTILGISGVKGDITGDFKVDEQDAYVTLMMLLGERVDTELERKVDMNNNG
IIELGDVLLILQES

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=486, Percent_Identity=28.6008230452675, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI157266296, Length=486, Percent_Identity=28.8065843621399, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI157266292, Length=472, Percent_Identity=28.8135593220339, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI116734717, Length=329, Percent_Identity=32.2188449848024, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI294660770, Length=325, Percent_Identity=31.6923076923077, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI294660772, Length=286, Percent_Identity=32.1678321678322, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI48994877, Length=332, Percent_Identity=34.3373493975904, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320689, Length=461, Percent_Identity=32.9718004338395, Blast_Score=173, Evalue=4e-44,
Organism=Drosophila melanogaster, GI21358067, Length=438, Percent_Identity=29.2237442922374, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI18859923, Length=293, Percent_Identity=36.8600682593857, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21355149, Length=333, Percent_Identity=32.1321321321321, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19921912, Length=330, Percent_Identity=33.030303030303, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21355981, Length=327, Percent_Identity=31.8042813455657, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24651554, Length=340, Percent_Identity=32.0588235294118, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24585213, Length=332, Percent_Identity=32.5301204819277, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI85725200, Length=322, Percent_Identity=29.8136645962733, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI85815833, Length=322, Percent_Identity=29.8136645962733, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21355151, Length=337, Percent_Identity=32.0474777448071, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24651556, Length=309, Percent_Identity=32.0388349514563, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24657835, Length=475, Percent_Identity=27.3684210526316, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24657827, Length=463, Percent_Identity=26.133909287257, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24657842, Length=464, Percent_Identity=26.2931034482759, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 53930; Mature: 53930

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00018 EF_HAND_1 ; PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITISSLLLLLLISTNLNLAYDSQESGIKNIIILIGDGMGMSHVQITKLVYGHLNMEE
CCEEEHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCHHEEEHHHHHCCCCCC
FPIIGFELTESLSGEVTDSAAAGTAIATGVKTYNRMISVTNITGKVTNLTTLLEIAQVLG
CCEEEHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHC
KSTGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIAHRVNVLLGGGRKKFDENTLKMA
CCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHH
KEQGYNIVFTKEELEKAEGEFILGLFADSHIPYVLDRKPEDVGLLEMTKKAISILEKNPN
HHCCCEEEEEHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCC
GFFLMIEGGRIDHAAHENDIASVVAETKEFDDVVGYVLEYAKKRGDTLVIVLADHETGGL
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
GLGLTYGDAINEDVIRNINASVSKIASEIRATNDIKRVIKKYTGFELTEDEINYIEEAIN
EEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LADEYALQNAIADIINKRVGVGFVSHKHTGAPVSLLAYGPGAENFAGFLHHVDTAKLIAK
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHH
LMLFGKKDIPVTILGISGVKGDITGDFKVDEQDAYVTLMMLLGERVDTELERKVDMNNNG
HHHHCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCCCC
IIELGDVLLILQES
EEEECCEEEEEECC
>Mature Secondary Structure
MKKITISSLLLLLLISTNLNLAYDSQESGIKNIIILIGDGMGMSHVQITKLVYGHLNMEE
CCEEEHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCHHEEEHHHHHCCCCCC
FPIIGFELTESLSGEVTDSAAAGTAIATGVKTYNRMISVTNITGKVTNLTTLLEIAQVLG
CCEEEHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHC
KSTGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIAHRVNVLLGGGRKKFDENTLKMA
CCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHH
KEQGYNIVFTKEELEKAEGEFILGLFADSHIPYVLDRKPEDVGLLEMTKKAISILEKNPN
HHCCCEEEEEHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCC
GFFLMIEGGRIDHAAHENDIASVVAETKEFDDVVGYVLEYAKKRGDTLVIVLADHETGGL
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
GLGLTYGDAINEDVIRNINASVSKIASEIRATNDIKRVIKKYTGFELTEDEINYIEEAIN
EEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LADEYALQNAIADIINKRVGVGFVSHKHTGAPVSLLAYGPGAENFAGFLHHVDTAKLIAK
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHH
LMLFGKKDIPVTILGISGVKGDITGDFKVDEQDAYVTLMMLLGERVDTELERKVDMNNNG
HHHHCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCCCC
IIELGDVLLILQES
EEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]