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The map label for this gene is lysS [H]

Identifier: 18311447

GI number: 18311447

Start: 2820866

End: 2822371

Strand: Reverse

Name: lysS [H]

Synonym: CPE2465

Alternate gene names: 18311447

Gene position: 2822371-2820866 (Counterclockwise)

Preceding gene: 18311448

Following gene: 18311446

Centisome position: 93.1

GC content: 32.47

Gene sequence:

>1506_bases
ATGTCAAATGAGGAAATAAATATTCACGAAGCAGAAGAACAATTAAGTGAACAAGAGATGTTAAGAAGACAAAAGCTTGT
TGAATTACAAGAAGCTGGTAAGGACCCATTTGATGTATACAAGGTAGAGAGAACACACTCTTCTGCTGATGTAAAAGATA
ATTTTGAAGAGTTAGAAGGTAAAGAAGTAAAAGTTGCTGGAAGACTTATGTCAAAAAGAGGTCAAGGTAAAGTAGTATTC
GCTGATTTAGCAGATTTACCAGGAAAAATTCAATTATTTATTAAGATTGATAACGTTGGAGAGGAAGCTTTAAAGGAATT
TAAAACTTTCGATTTAGGTGACTGGGTTGCTGCAACTGGAGAAGTATTTAAAACAAAAATGGGTGAAGTTTCAGTAAAAG
TAACTTCATTTGAATTAATCTGTAAATCTTTAAAACCATTACCAGAAAAATGGCATGGTTTAAAAGACCCAGACTTAAGA
TACAGACAAAGAGAAGTTGATATAATAACAAACCCAGAAGTTAAAGATACTTTTATTAAGAGATCACAAATAGTAAAAGC
AATAAGAGAATTCTTAGATAATAGAGGATTCTTAGAGGTTGACACACCAATTCTTTCACCAATAGCTGGTGGAGCTGCTG
CTAGACCTTTCATAACTCACCACAATGCTTTAGATATAGATATGTATTTAAGAATTGCTACTGAGTTATACTTAAAGAGA
TTAATAGTTGCTGGATTTGAAAAAGTTTATGAAATGGGTAAAAACTTCAGAAATGAAGGGGTTTCAGTAAGACATAATCC
AGAATTCACTGCTATAGAGTTATATGAAGCATATGCTGATTACAATGACATGATGGAAATCATGGAAAACATGATTGCTT
ATGTTTGTGAAAAAGTAAATGGATCAACTAAAGTTACTTATGAAGGAACTGAAATAGACTTCACACCACCATGGAGAAGA
ATCACTATGGTTGATGCAGTTAAAGAATTTGCTGGTATAGATTTCAACGAAATTAAGAGTGATGAAGAAGCTCAAGCTAT
AGCTAAAGAGAAAAACTTAGAATTCCCTAAACCATTAGATAAGGTTACTAAAGGTGAAGTTCTAAATATGTTATTTGAAG
AATATGGTGAAGATAAATTAATCCAACCTACATTCTTAATAGATTATCCAGTAGAGATATCACCTCTTACTAAAAAGAAA
AGAGGAAATGAAATGTTTACTGAGAGATTTGAAGGATTTGTATATGGTAGAGAAGTATGTAACGCATACTCAGAGCTTAA
CGATCCAATAGTTCAAAGAGAAAGATTTGAACAACAAGCTAGAGAAAGAGAATACGGAGATGATGAAGCATACATGTTAG
ATGAAGAATTCATGAGTGCTTTAGAAACTGGTATGCCTCCAACTGGTGGATTAGGAATAGGAATAGACAGAATGATAATG
TTCTTAACTGACTCTTCATCAATAAGAGACGTTATATTATTCCCAACAATGAAACCACAAAAATAG

Upstream 100 bases:

>100_bases
AAAGTTGGAGATGTTGTAGAGATAGAAGTACCAGGAGGAACTACTAAGTTCGAAGTTCTTGGAATAAGAAGATAGATAAT
ATAGATGGAGGGATTTTTAA

Downstream 100 bases:

>100_bases
TTTATATAAAGTAGAAGTTTACTAAAAAGTAAACTTCTATTTTTTATTTTATTTGAAGAGAAAAAGCCTAAAAAAATTCT
AGATTATTTTTAAAATCTAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVERTHSSADVKDNFEELEGKEVKVAGRLMSKRGQGKVVF
ADLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATGEVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLR
YRQREVDIITNPEVKDTFIKRSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKR
LIVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVNGSTKVTYEGTEIDFTPPWRR
ITMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLDKVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKK
RGNEMFTERFEGFVYGREVCNAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIM
FLTDSSSIRDVILFPTMKPQK

Sequences:

>Translated_501_residues
MSNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVERTHSSADVKDNFEELEGKEVKVAGRLMSKRGQGKVVF
ADLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATGEVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLR
YRQREVDIITNPEVKDTFIKRSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKR
LIVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVNGSTKVTYEGTEIDFTPPWRR
ITMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLDKVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKK
RGNEMFTERFEGFVYGREVCNAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIM
FLTDSSSIRDVILFPTMKPQK
>Mature_500_residues
SNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVERTHSSADVKDNFEELEGKEVKVAGRLMSKRGQGKVVFA
DLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATGEVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLRY
RQREVDIITNPEVKDTFIKRSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKRL
IVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVNGSTKVTYEGTEIDFTPPWRRI
TMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLDKVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKKR
GNEMFTERFEGFVYGREVCNAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIMF
LTDSSSIRDVILFPTMKPQK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=502, Percent_Identity=43.8247011952191, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI194272210, Length=502, Percent_Identity=43.8247011952191, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI45439306, Length=460, Percent_Identity=23.2608695652174, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI40789249, Length=265, Percent_Identity=25.2830188679245, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789256, Length=509, Percent_Identity=48.7229862475442, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1790571, Length=488, Percent_Identity=49.3852459016393, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=28.7878787878788, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=25.6916996047431, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=525, Percent_Identity=42.2857142857143, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535927, Length=525, Percent_Identity=42.2857142857143, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71994340, Length=510, Percent_Identity=43.1372549019608, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551876, Length=354, Percent_Identity=25.1412429378531, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=467, Percent_Identity=44.5396145610278, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324256, Length=482, Percent_Identity=32.1576763485477, Blast_Score=203, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323011, Length=473, Percent_Identity=22.8329809725159, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=242, Percent_Identity=29.7520661157025, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640849, Length=518, Percent_Identity=42.8571428571429, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24640851, Length=518, Percent_Identity=42.8571428571429, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=21.9088937093275, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57550; Mature: 57419

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVERTHSSADVKDNFEELEG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCHHHHHHHHCC
KEVKVAGRLMSKRGQGKVVFADLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATG
CEEHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHH
EVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLRYRQREVDIITNPEVKDTFIK
HHHHHHCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCEEEECCCCHHHHHHH
RSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVN
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GSTKVTYEGTEIDFTPPWRRITMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLD
CCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCCCCHH
KVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKKRGNEMFTERFEGFVYGREVC
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIM
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHHHEEE
FLTDSSSIRDVILFPTMKPQK
EEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVERTHSSADVKDNFEELEG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCHHHHHHHHCC
KEVKVAGRLMSKRGQGKVVFADLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATG
CEEHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHH
EVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLRYRQREVDIITNPEVKDTFIK
HHHHHHCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCEEEECCCCHHHHHHH
RSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVN
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GSTKVTYEGTEIDFTPPWRRITMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLD
CCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCCCCHH
KVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKKRGNEMFTERFEGFVYGREVC
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIM
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHHHEEE
FLTDSSSIRDVILFPTMKPQK
EEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA