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The map label for this gene is greA

Identifier: 18311448

GI number: 18311448

Start: 2822397

End: 2822873

Strand: Reverse

Name: greA

Synonym: CPE2466

Alternate gene names: 18311448

Gene position: 2822873-2822397 (Counterclockwise)

Preceding gene: 18311449

Following gene: 18311447

Centisome position: 93.12

GC content: 31.87

Gene sequence:

>477_bases
ATGAGCGAAAAGAAACATATAATGACATATGAAGGTGTTAAAAAATTAGAAGATGAATTAGAATATCTTAAAACAGTAAA
AAGAAAAGAAATAACTGAAAAGATAAAAGTTGCTTTAGGATACGGAGATTTAAGTGAGAACTCAGAATATGATGAAGCTA
AAAATGAGCAAGCTTTCACTGAAGGAAGAATAATTCAATTAGAAAACATGCTTAAAAACGCTGTTGTTGTTGATGAGTCA
GAAATATCAACAGATATAGTTACAGTTGGATCAATCGTTAAGGTTATGGACTTTGATTTTGATGAAGAAGTTGAGTATTC
AATAGTTGGTTCAGCAGAAGCAGATCCAATGAACTTTAAGATATCAAATGAATCACCAGTTGGTGAAGGGTTAATGGGTA
AAAAAGTTGGAGATGTTGTAGAGATAGAAGTACCAGGAGGAACTACTAAGTTCGAAGTTCTTGGAATAAGAAGATAG

Upstream 100 bases:

>100_bases
ATAATTAGGCTTAATAATCTTAAAGTTTTTATGAATTGTATATTAACAGGTTAAGTTTAAATATGATATTATAATAATAT
GTTCAAAGGGGAGAAAGAAA

Downstream 100 bases:

>100_bases
ATAATATAGATGGAGGGATTTTTAAATGTCAAATGAGGAAATAAATATTCACGAAGCAGAAGAACAATTAAGTGAACAAG
AGATGTTAAGAAGACAAAAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDLSENSEYDEAKNEQAFTEGRIIQLENMLKNAVVVDES
EISTDIVTVGSIVKVMDFDFDEEVEYSIVGSAEADPMNFKISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR

Sequences:

>Translated_158_residues
MSEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDLSENSEYDEAKNEQAFTEGRIIQLENMLKNAVVVDES
EISTDIVTVGSIVKVMDFDFDEEVEYSIVGSAEADPMNFKISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR
>Mature_157_residues
SEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDLSENSEYDEAKNEQAFTEGRIIQLENMLKNAVVVDESE
ISTDIVTVGSIVKVMDFDFDEEVEYSIVGSAEADPMNFKISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=45.3333333333333, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=32.9032258064516, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CLOP1 (Q0TMI6)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697154.1
- ProteinModelPortal:   Q0TMI6
- SMR:   Q0TMI6
- STRING:   Q0TMI6
- GeneID:   4202837
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2782
- TIGR:   CPF_2782
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   CPER195103:CPF_2782-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17660; Mature: 17529

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDLSENSEYDEAKNEQAFT
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHCCCCHHC
EGRIIQLENMLKNAVVVDESEISTDIVTVGSIVKVMDFDFDEEVEYSIVGSAEADPMNFK
CCCEEEHHHHHHHCEEEECCHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEE
ISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR
ECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
SEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDLSENSEYDEAKNEQAFT
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHCCCCHHC
EGRIIQLENMLKNAVVVDESEISTDIVTVGSIVKVMDFDFDEEVEYSIVGSAEADPMNFK
CCCEEEHHHHHHHCEEEECCHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEE
ISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR
ECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA