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The map label for this gene is sigF [H]

Identifier: 18311030

GI number: 18311030

Start: 2339314

End: 2340069

Strand: Reverse

Name: sigF [H]

Synonym: CPE2048

Alternate gene names: 18311030

Gene position: 2340069-2339314 (Counterclockwise)

Preceding gene: 18311031

Following gene: 18311029

Centisome position: 77.19

GC content: 30.29

Gene sequence:

>756_bases
ATGGATATAAGTAGTATTGAGAAAGATAATTTTAATTATGACAAAAATACAACTCTCATAGTCATGGCTAAAGAAGGAAA
TAAGGAGGCAATGAATAAGCTTATAGAAGTTAATACTCCTTTAGTTTCTTCAATATGCAAGAGATTTACAAATAGAGGGT
ATGACTATGAGGATATATTTCAGATTGGGTCAATGGGATTAGTTAAAGCTATAAACAACTTTAATGATAGTTTTAATGTT
AAATTTTCAACTTATGCTGTGCCTATGATTATAGGTGAAATAAAGAGATTCTTAAGGGATGATGGAATAATAAAGGTAAG
TAGAAGCACTAAGACTTTAGCTAAAAAGCTTCATTACAACAAGGAAGAACTAATAAAAAAATTAAATAGGGATCCTACTG
TAGATGAATTAGCTGAATACTCTGGTGTTGATAAGGAAGAGGTAATAGTAGCCTTAGAATCAGCAAGTAGTATGCAATAT
TTATATGATACTATACACCATGATGAAGGAACGCCAGTTCTCTTAATAGATAAACTTAGTGAGAAGGGTGAAGATGATGA
CATGGTCAATAAAATAGCTTTAAGAGAAGCTATTAGTTCATTAGATTCAAAGGGTAAGCAAATCATAATGCTTAGGTATT
TCAAGGATAAAACTCAAATTCAAGTTGCAAAAATGCTTGGTATAAGTCAAGTTCAAGTTTCTAGAATAGAGAAAAAGGTA
CTAAAAATTATGAAGGAAAAATTAGAAGAGGATTGA

Upstream 100 bases:

>100_bases
AAACATTCATGGATTCTTTAGAAGTTTATAGTGAAAAGGATAAGGGTACAAAAATAATAATGAAGAAAAAAATGAATACA
TAGGCAGGTGAATAAAGTCT

Downstream 100 bases:

>100_bases
TTATAAATGAAATGTTTTAGGAGCATTATTTTATGTGAAAGTAATCATATAAAATAATGCTCATTTTTTTGTATAGATTA
AAAATAAGAACAAATAATAT

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSSICKRFTNRGYDYEDIFQIGSMGLVKAINNFNDSFNV
KFSTYAVPMIIGEIKRFLRDDGIIKVSRSTKTLAKKLHYNKEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQY
LYDTIHHDEGTPVLLIDKLSEKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKTQIQVAKMLGISQVQVSRIEKKV
LKIMKEKLEED

Sequences:

>Translated_251_residues
MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSSICKRFTNRGYDYEDIFQIGSMGLVKAINNFNDSFNV
KFSTYAVPMIIGEIKRFLRDDGIIKVSRSTKTLAKKLHYNKEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQY
LYDTIHHDEGTPVLLIDKLSEKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKTQIQVAKMLGISQVQVSRIEKKV
LKIMKEKLEED
>Mature_251_residues
MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSSICKRFTNRGYDYEDIFQIGSMGLVKAINNFNDSFNV
KFSTYAVPMIIGEIKRFLRDDGIIKVSRSTKTLAKKLHYNKEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQY
LYDTIHHDEGTPVLLIDKLSEKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKTQIQVAKMLGISQVQVSRIEKKV
LKIMKEKLEED

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789098, Length=254, Percent_Identity=26.3779527559055, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28704; Mature: 28704

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSSICKRFTNRGYDYEDIF
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
QIGSMGLVKAINNFNDSFNVKFSTYAVPMIIGEIKRFLRDDGIIKVSRSTKTLAKKLHYN
HHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCC
KEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQYLYDTIHHDEGTPVLLIDKLS
HHHHHHHHCCCCCHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECCC
EKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKTQIQVAKMLGISQVQVSRIEKKV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHH
LKIMKEKLEED
HHHHHHHHCCC
>Mature Secondary Structure
MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSSICKRFTNRGYDYEDIF
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
QIGSMGLVKAINNFNDSFNVKFSTYAVPMIIGEIKRFLRDDGIIKVSRSTKTLAKKLHYN
HHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCC
KEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQYLYDTIHHDEGTPVLLIDKLS
HHHHHHHHCCCCCHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECCC
EKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKTQIQVAKMLGISQVQVSRIEKKV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHH
LKIMKEKLEED
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]