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The map label for this gene is sigA [H]

Identifier: 18310989

GI number: 18310989

Start: 2298082

End: 2299203

Strand: Reverse

Name: sigA [H]

Synonym: CPE2007

Alternate gene names: 18310989

Gene position: 2299203-2298082 (Counterclockwise)

Preceding gene: 18310990

Following gene: 18310988

Centisome position: 75.85

GC content: 32.26

Gene sequence:

>1122_bases
ATGGCGGAAGGAGGTAATAAAATGAAAGCTAAAACAACAAAAGCAAAAAAAGGTGAAGGTAAAGAAAAAGTTGACAAAAT
GGTCTTAGTAAAAAGACTTATAGATAAAGGTAAAAAAAGCGGTTCATTGACTTACAAGGAGATAATGGATGAGCTTGATG
AAATAGAATTAAACCCAGAACAAATAGAGAAAATCTATGAGGTTCTAGAATCAATGGGTATAGAGGTTATAAGTGAAATC
GAGCAAGAAGAGGAAGAGGAGGAAGAATTAGATCTTTCTGTTCCAGAAGGTATTGCTATTGATGACCCTGTAAGAATGTA
CTTAAAAGAAATAGGTAAAGTTCCACTATTATCATCAGAGGATGAAATAGAGCTTGCTAAAAAAATAGAAGAAGGAAGCA
ACTATGCTAAGAAAAAATTAGCAGAGGCTAACTTAAGACTTGTTGTAAGTATAGCTAAAAGATATGTTGGTAGAGGAATG
TTATTCCTAGATCTTATACAAGAAGGTAACTTAGGTCTTATAAAGGCTGTTGAAAAATTTGATTACAGAAAAGGATATAA
GTTCTCAACATATGCTACATGGTGGATAAGACAGGCAATAACTAGAGCTATTGCTGATCAAGCAAGAACTATAAGAATAC
CAGTTCATATGGTAGAAACTATAAATAAACTTATAAGAATACAAAGACAATTAGTTCAAGAGTTAGGAAGAGATCCATTA
CCAGAGGAATTATCAAAACAAATGGATATGCCAGTAGATAAGGTAAGAGAAATCTTAAAAATAGCTCAAGAACCAGTTTC
ATTAGAAACTCCAATTGGTGAAGAGGAAGATTCACATTTAGGTGACTTTATACCAGATGATGATGCTCCAGCACCAGCAG
AAGCAGCAGCATTTACAATGTTAAAAGAACAATTAATAAATGTTTTAGATACTTTAACTCCTAGAGAGGAAAAAGTATTA
AGATTAAGATTTGGATTAGATGATGGAAGAGCTAGAACTCTTGAAGAAGTTGGTAAAGAATTCAACGTAACTAGAGAGAG
AATTAGACAAATTGAAGCGAAAGCTTTAAGAAAATTAAGACATCCAAGTAGAAGTAAAAAGTTAAAAGATTATTTAGATT
AG

Upstream 100 bases:

>100_bases
GTGAACAAAACAATATGTTTAAAGAGTCACTTGATTTAGTAAAAAGAGCTAAAGAAATAAGTGATAAATTAAAAGAACTA
GAAAGGAGTAAGTAGTTTTA

Downstream 100 bases:

>100_bases
TATCTTAAGATTAGTGCCTTAAAGGTGCTAATCTTTTTTTAGTTAATATTTAAAAAAGTTTTAATGTTATATAAGATGAG
AAGTTTAAAATTATATGAAA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A; Major vegetative sigma factor [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MAEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLTYKEIMDELDEIELNPEQIEKIYEVLESMGIEVISEI
EQEEEEEEELDLSVPEGIAIDDPVRMYLKEIGKVPLLSSEDEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLVQELGRDPL
PEELSKQMDMPVDKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAFTMLKEQLINVLDTLTPREEKVL
RLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDYLD

Sequences:

>Translated_373_residues
MAEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLTYKEIMDELDEIELNPEQIEKIYEVLESMGIEVISEI
EQEEEEEEELDLSVPEGIAIDDPVRMYLKEIGKVPLLSSEDEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLVQELGRDPL
PEELSKQMDMPVDKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAFTMLKEQLINVLDTLTPREEKVL
RLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDYLD
>Mature_372_residues
AEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLTYKEIMDELDEIELNPEQIEKIYEVLESMGIEVISEIE
QEEEEEEELDLSVPEGIAIDDPVRMYLKEIGKVPLLSSEDEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGML
FLDLIQEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLVQELGRDPLP
EELSKQMDMPVDKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAFTMLKEQLINVLDTLTPREEKVLR
LRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=69.327731092437, Blast_Score=350, Evalue=8e-98,
Organism=Escherichia coli, GI1789098, Length=304, Percent_Identity=43.0921052631579, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=28.9377289377289, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42685; Mature: 42554

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLTYKEIMDELDEIELNPE
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
QIEKIYEVLESMGIEVISEIEQEEEEEEELDLSVPEGIAIDDPVRMYLKEIGKVPLLSSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
DEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
DYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLVQELGRDPL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEELSKQMDMPVDKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAFTM
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQLINVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLR
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKKLKDYLD
CCHHHHHHHHHCC
>Mature Secondary Structure 
AEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLTYKEIMDELDEIELNPE
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
QIEKIYEVLESMGIEVISEIEQEEEEEEELDLSVPEGIAIDDPVRMYLKEIGKVPLLSSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
DEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
DYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLVQELGRDPL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEELSKQMDMPVDKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAFTM
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQLINVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLR
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKKLKDYLD
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]