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The map label for this gene is dnaG [H]

Identifier: 18310990

GI number: 18310990

Start: 2299209

End: 2300996

Strand: Reverse

Name: dnaG [H]

Synonym: CPE2008

Alternate gene names: 18310990

Gene position: 2300996-2299209 (Counterclockwise)

Preceding gene: 18310991

Following gene: 18310989

Centisome position: 75.9

GC content: 28.75

Gene sequence:

>1788_bases
TTGCGAATTTCAGAAGAAATTATAGAAAAAGTTAAAGAGCAGAACGATATTGTTGATGTTGTTTCTGATGTGGTAAGACT
AAAAAGAGCTGGAAGAAATTTTTCTGGTTTATGTCCATTCCATAATGAAAAAAGTCCATCTTTTAGTGTTTCTCCTGACA
AACAAATATTTAAGTGCTTCGGATGTGGGGAAGCTGGAAATGTAATAAGTTTTGTTATGAAAACTAAAAATTTAAATTTT
GTAGATGCTGTTAAAGAGTTAGCTGATAGGGCTAATATAATAATACCTATAGAAGATGGTAAGCAAAGCGAATCTCAAAA
GAAAAAGCAGGTTTTATATAACATAAATAAGCAAGCTGCTAGATATTACTTTTCTAATCTTCAAAATAATAAGGAAGCTA
AGGAATATTTTTTAAGAAGGGGAATAAAAGAAAATATATTAAAAAGCTTTGGTCTTGGATACGCTAAGGAAGGATGGCAA
AATACCATAAACCATCTTAGAAAACAAGGCTTTAGTAATGAAGCTATTGAGGCAGCTGGATTAGGGATTAAGAGTGAAAA
GGGAACTTTTTATGATAGATTCAGAAATAGAGTTATTTTCCCTGTATTTGACTATAAAGGAAGTGTAATAGGTTTTGGTG
GTAGAGTTTTAGATGACTCTAAGCCAAAATATTTAAACTCTCCAGAAACATTAGTCTTTCACAAAGGAACTAATCTATAT
GCTTTAAATTTTGCTATAAAGCATGGATTACAGGAAAGAACATTAATAATAGTTGAAGGATATATGGATTGTATATCACT
TCATCAATATGGAATAACTAATGTAGTTGCTTCTTTAGGTACTGCATTGACAGACAATCAAGCTAGATTATTAAAAAGGT
ATGTAGACAGAGTAATAATATCCTATGACGCAGATATTGCTGGGCAAATGGCTACTATACGTGGACTTGAGATATTGCAA
AATGCAGGATTAGATGTTAGAGTACTAACTGTACCAAAAGGCAAAGATCCAGATGAATTTATAAGAGCTAATGGTAAAGA
GGCTTTTATAAAGTTAATTCAATCTGCAAAAAAACTTATAGATTATAAGCTTCATAGGGCTAGAATTGGTATAGATTTTT
CAGATAATAATGGATTAATAAAATATGCTGAAAGAGTTGTTAATATACTATCAGATTTAAATCCTATAGAAAAAGATGTC
TACATAAAGAGGATTTCAGAAGAAACATCCATAAAAGAACAAGCATTTTATGATTTATTAAATAAAAACTTAATAAATAG
TAATAAAAACCATGAAAATGTGAATAATAAGGAAGAATATGGAACAAAATTATATGTAGAACCTCCTTATGTTAAATGTG
AAAGAAGTGTTTTAAAACTTCTTTGTATGGAGGAAAACCATTCATTAGTAAAGAAATACTTAGAAGATGATGATTTAATA
TCTCCTAATATTAAATCCTTAGAAAAGATAATCAATGATTCAATTGAGGAAATAGGATTTAATAACTTAGGAAAACATGT
AGAAGCTAAGTGTGAAGATCCAAAAGTTTTAAAGGAATGGTTAGAAGTAAAAGACTTACTTATTCTAGATCATTCTTCTA
ATATAGAAGTTCTTGTTAGAGATTATATTAATCTTATTAGAAAATATAAGTTAGAACAGGAACAGACTACTGTACTTAAA
GAATTAAAAAGATGTGAACAAAACAATATGTTTAAAGAGTCACTTGATTTAGTAAAAAGAGCTAAAGAAATAAGTGATAA
ATTAAAAGAACTAGAAAGGAGTAAGTAG

Upstream 100 bases:

>100_bases
TTTAAAAAAAAAGTTATATTTAAAATTTGAGAAGGATTTTATCAATTGATGTCGAATAATAATTAATTGTCAAAAAATAT
TAAGAAGGTGAGTAGATTTC

Downstream 100 bases:

>100_bases
TTTTAATGGCGGAAGGAGGTAATAAAATGAAAGCTAAAACAACAAAAGCAAAAAAAGGTGAAGGTAAAGAAAAAGTTGAC
AAAATGGTCTTAGTAAAAAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEKSPSFSVSPDKQIFKCFGCGEAGNVISFVMKTKNLNF
VDAVKELADRANIIIPIEDGKQSESQKKKQVLYNINKQAARYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQ
NTINHLRKQGFSNEAIEAAGLGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLDDSKPKYLNSPETLVFHKGTNLY
ALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVVASLGTALTDNQARLLKRYVDRVIISYDADIAGQMATIRGLEILQ
NAGLDVRVLTVPKGKDPDEFIRANGKEAFIKLIQSAKKLIDYKLHRARIGIDFSDNNGLIKYAERVVNILSDLNPIEKDV
YIKRISEETSIKEQAFYDLLNKNLINSNKNHENVNNKEEYGTKLYVEPPYVKCERSVLKLLCMEENHSLVKKYLEDDDLI
SPNIKSLEKIINDSIEEIGFNNLGKHVEAKCEDPKVLKEWLEVKDLLILDHSSNIEVLVRDYINLIRKYKLEQEQTTVLK
ELKRCEQNNMFKESLDLVKRAKEISDKLKELERSK

Sequences:

>Translated_595_residues
MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEKSPSFSVSPDKQIFKCFGCGEAGNVISFVMKTKNLNF
VDAVKELADRANIIIPIEDGKQSESQKKKQVLYNINKQAARYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQ
NTINHLRKQGFSNEAIEAAGLGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLDDSKPKYLNSPETLVFHKGTNLY
ALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVVASLGTALTDNQARLLKRYVDRVIISYDADIAGQMATIRGLEILQ
NAGLDVRVLTVPKGKDPDEFIRANGKEAFIKLIQSAKKLIDYKLHRARIGIDFSDNNGLIKYAERVVNILSDLNPIEKDV
YIKRISEETSIKEQAFYDLLNKNLINSNKNHENVNNKEEYGTKLYVEPPYVKCERSVLKLLCMEENHSLVKKYLEDDDLI
SPNIKSLEKIINDSIEEIGFNNLGKHVEAKCEDPKVLKEWLEVKDLLILDHSSNIEVLVRDYINLIRKYKLEQEQTTVLK
ELKRCEQNNMFKESLDLVKRAKEISDKLKELERSK
>Mature_595_residues
MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEKSPSFSVSPDKQIFKCFGCGEAGNVISFVMKTKNLNF
VDAVKELADRANIIIPIEDGKQSESQKKKQVLYNINKQAARYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQ
NTINHLRKQGFSNEAIEAAGLGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLDDSKPKYLNSPETLVFHKGTNLY
ALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVVASLGTALTDNQARLLKRYVDRVIISYDADIAGQMATIRGLEILQ
NAGLDVRVLTVPKGKDPDEFIRANGKEAFIKLIQSAKKLIDYKLHRARIGIDFSDNNGLIKYAERVVNILSDLNPIEKDV
YIKRISEETSIKEQAFYDLLNKNLINSNKNHENVNNKEEYGTKLYVEPPYVKCERSVLKLLCMEENHSLVKKYLEDDDLI
SPNIKSLEKIINDSIEEIGFNNLGKHVEAKCEDPKVLKEWLEVKDLLILDHSSNIEVLVRDYINLIRKYKLEQEQTTVLK
ELKRCEQNNMFKESLDLVKRAKEISDKLKELERSK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=410, Percent_Identity=38.0487804878049, Blast_Score=307, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68212; Mature: 68212

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEKSPSFSVSPDKQIFKCF
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GCGEAGNVISFVMKTKNLNFVDAVKELADRANIIIPIEDGKQSESQKKKQVLYNINKQAA
CCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQNTINHLRKQGFSNEAIEAAG
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHC
LGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLDDSKPKYLNSPETLVFHKGTNLY
CCCCCCCCHHHHHHHCCEEEEEECCCCCEEECCCEECCCCCCCCCCCCCEEEEECCCCEE
ALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVVASLGTALTDNQARLLKRYVDRVII
EEHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SYDADIAGQMATIRGLEILQNAGLDVRVLTVPKGKDPDEFIRANGKEAFIKLIQSAKKLI
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHH
DYKLHRARIGIDFSDNNGLIKYAERVVNILSDLNPIEKDVYIKRISEETSIKEQAFYDLL
HHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NKNLINSNKNHENVNNKEEYGTKLYVEPPYVKCERSVLKLLCMEENHSLVKKYLEDDDLI
HHHHHCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
SPNIKSLEKIINDSIEEIGFNNLGKHVEAKCEDPKVLKEWLEVKDLLILDHSSNIEVLVR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHH
DYINLIRKYKLEQEQTTVLKELKRCEQNNMFKESLDLVKRAKEISDKLKELERSK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEKSPSFSVSPDKQIFKCF
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GCGEAGNVISFVMKTKNLNFVDAVKELADRANIIIPIEDGKQSESQKKKQVLYNINKQAA
CCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQNTINHLRKQGFSNEAIEAAG
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHC
LGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLDDSKPKYLNSPETLVFHKGTNLY
CCCCCCCCHHHHHHHCCEEEEEECCCCCEEECCCEECCCCCCCCCCCCCEEEEECCCCEE
ALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVVASLGTALTDNQARLLKRYVDRVII
EEHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SYDADIAGQMATIRGLEILQNAGLDVRVLTVPKGKDPDEFIRANGKEAFIKLIQSAKKLI
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHH
DYKLHRARIGIDFSDNNGLIKYAERVVNILSDLNPIEKDVYIKRISEETSIKEQAFYDLL
HHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NKNLINSNKNHENVNNKEEYGTKLYVEPPYVKCERSVLKLLCMEENHSLVKKYLEDDDLI
HHHHHCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
SPNIKSLEKIINDSIEEIGFNNLGKHVEAKCEDPKVLKEWLEVKDLLILDHSSNIEVLVR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHH
DYINLIRKYKLEQEQTTVLKELKRCEQNNMFKESLDLVKRAKEISDKLKELERSK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]