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The map label for this gene is phsB [H]

Identifier: 18310778

GI number: 18310778

Start: 2075199

End: 2075612

Strand: Reverse

Name: phsB [H]

Synonym: CPE1796

Alternate gene names: 18310778

Gene position: 2075612-2075199 (Counterclockwise)

Preceding gene: 18310779

Following gene: 18310777

Centisome position: 68.47

GC content: 33.57

Gene sequence:

>414_bases
TTGAAAAGAATAAAAATCAATAGAGATAAATGCGTGGGATGCTTAACATGCACAACTGCTTGTATAGTTTCTCATGATTC
AGAGGATACAAGAAGTAGAATTGCAATAACAAGTGAAGGGAAATATACTCCTATTTTCTGTAGACACTGTGATAGACCAG
AGTGTGTTTACACTTGTATGAGTGGTGCTATGAAGAAAGATAAAGAAACTGGTTTTGTAACTTATGACAAATCAAGATGT
GCAAGTTGTTTTATGTGTGTAATGGCTTGCCCATATGGAGTATTAAAAGCTGAACATGAAATGAATAAATATATAATGAA
ATGTGATATGTGTGCAAGTACAAAAGAAAAAACTCCTCAATGCGTTGCAAAATGTCCAATGGGAGCAATAACTTTAGAGG
AGGTAGAAGAATAA

Upstream 100 bases:

>100_bases
TTTATTAACACCATCTGTATTTGATCCTTATTCTAAGGAGCCTTCATATAAAACAGTTCAAGTTAATATAGAAAAGGTTA
GAAAATAGGAGGGGATTATT

Downstream 100 bases:

>100_bases
TATGAGATATATTGTTGTGGGGGCATCAGCTGCTGGAATAAGTGGAGCCAAAACACTTAGAGAACTTGACAAAGATGCAG
AAATAATATTAGTTTCAAAG

Product: nitrate reductase electron transfer subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTPIFCRHCDRPECVYTCMSGAMKKDKETGFVTYDKSRC
ASCFMCVMACPYGVLKAEHEMNKYIMKCDMCASTKEKTPQCVAKCPMGAITLEEVEE

Sequences:

>Translated_137_residues
MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTPIFCRHCDRPECVYTCMSGAMKKDKETGFVTYDKSRC
ASCFMCVMACPYGVLKAEHEMNKYIMKCDMCASTKEKTPQCVAKCPMGAITLEEVEE
>Mature_137_residues
MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTPIFCRHCDRPECVYTCMSGAMKKDKETGFVTYDKSRC
ASCFMCVMACPYGVLKAEHEMNKYIMKCDMCASTKEKTPQCVAKCPMGAITLEEVEE

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=151, Percent_Identity=35.0993377483444, Blast_Score=79, Evalue=9e-17,
Organism=Escherichia coli, GI87082179, Length=139, Percent_Identity=32.3741007194245, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788811, Length=143, Percent_Identity=29.3706293706294, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI87082180, Length=139, Percent_Identity=31.6546762589928, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787122, Length=151, Percent_Identity=33.112582781457, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=140, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1789067, Length=159, Percent_Identity=34.5911949685535, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=40.4040404040404, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367245, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI226510944, Length=140, Percent_Identity=32.8571428571429, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 15403; Mature: 15403

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.7 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
18.2 %Cys+Met (Translated Protein)
11.7 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
18.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTPIFCRHCDRPECVYTCM
CCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHH
SGAMKKDKETGFVTYDKSRCASCFMCVMACPYGVLKAEHEMNKYIMKCDMCASTKEKTPQ
HHHHCCCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
CVAKCPMGAITLEEVEE
HHHHCCCCCEEHHHHCC
>Mature Secondary Structure
MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTPIFCRHCDRPECVYTCM
CCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHH
SGAMKKDKETGFVTYDKSRCASCFMCVMACPYGVLKAEHEMNKYIMKCDMCASTKEKTPQ
HHHHCCCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
CVAKCPMGAITLEEVEE
HHHHCCCCCEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]