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The map label for this gene is narB [H]

Identifier: 18310779

GI number: 18310779

Start: 2075625

End: 2077703

Strand: Reverse

Name: narB [H]

Synonym: CPE1797

Alternate gene names: 18310779

Gene position: 2077703-2075625 (Counterclockwise)

Preceding gene: 18310780

Following gene: 18310778

Centisome position: 68.54

GC content: 31.79

Gene sequence:

>2079_bases
ATGAAAAGAATACAATCTACTTGTAACTATTGTGCTCTTGCGTGTAATTTAGATTTTTACACAGAGGATGGAAAGATAAA
AAGAGTAGTTCCAACTCCACATTATCCAGTTAATAAGGGATTTTCTTGTATAAAAGGACTTAATTTAGATAAACAATGTA
CTAAGTTTAATGGTTCAAAGAAGCCTTTACTTAAAATGAAAGATGGAGAAAGAAAAGCTATAGAATGGAAAGAAGCTTTT
GATTTATTTGCTAGTAAGATGACAGCTATTCAAGAAAAGTATGGTAAAGAAAGTGTAGCTTACATAAGTACAGGTCAATT
ACCAACAGAGGAAATGGCTCTTTTAGGTCATGTAGGAAGAAGCTACATGGGTATAAATGGAGATGGTAATACAAGACTTT
GTATGGCATCAGCAGTTGTAGCTTACAAACAAAGCTTTGGATTTGACGCACCTCCATATACTTTAAAAGATTTAGAGCTT
TCAGATACTATATTTTTTATTGGAGCAAATCCAGTTATAGCTCATCCAATAGCTTGGGGAAGAGTTAGAAAAAATAAGGA
TGCTAAAATAATTACTATAGATCCAAGAAAATCTGAGACAGCTATGAATTCAGATATGTGGATTGATATAAAAACTAAGG
GAGATTTAGCTCTTTTCTATACTTTAGCAAATGTTCTTATAGAAAAAGGATGGATAGACCAAGATTATATAAATAATTAC
ACAGAGGGCTTTGAAGATTTCAAAGCACATGTTAAGAAATATACATTAGAAGATGTTGAAGAAAGAACAGGAATCTCTAA
GATGAGAGTTCTAGAACTTGCAAAAATAATCCATGAAGGAAAGAGAGTTTCATTTTGGTGGACAATGGGAGTTAACCAAA
GCTATGAAGCTGTTAGAACTGCTCAAGCCATTATAAACCTTGCTTTAATCACAGGAAATATGGGAAGAGAAGGAACAGGA
GCTAACTCTTTAACAGGACAATGTAATGCCATGGGATCAAGAATGTTTAGTAATACAACTGCTCTTTATGGTGGTGGAGA
ATACAATAACAAAGAGAGAAGAAAAGTAGTTGCTGATATATTAGGCATGGATGAAAATATGCTTCCAAGTAAGCCTACTT
TAGATTATGAACAAATAATAAAAGGAATAAATAAAGAAGAAATCAAAGGATTATGGGTAATCTGTACTAATCCTAGACAT
TCATTTAGTAACAACGAAGAGTTTAAAAAAGCTATGAAAAACCTAGATTTCTTTGTAGTTCAAGATATTTATGAAGATAC
AGATAGTTCTAAAGAATGTGATTTATATTTACCTTCAGTTCCAGCTATTAAAAAAGAAGGTTTCTTAATAAACACTGAGC
GTAGACTTTCAGCTATGGTTCCTGTTTTAGAGAAGGAAGAAGATGAATTAAGTGATTATGAAATATTCTTAGGAATTGGA
GAAGCCTTAGGAATGGGAAGTCTTTTAGACAAATGGAGAACTCCAAAGGATGCTTTTAATCTTTTAAGAGAATGTAGTAA
AGGAATGCCTTGTGATATTACGGGAGTAACTTATGAGAGATTAAGAGGTTCTAAGGGAATTCAATGGCCTTGTAGAGAAG
GAGAAGAATTAGAGGCTGATGAAAGAAGATTATTTGAAGACGGAAAATACTATACTCCAAGTGGAAAAGCTAAGTTTATC
TTTGAAGATGTAACTGAAAATCCAAATGCTACAAACGAAGAGTTTCCATTTAATTTAAATACTGGTAGAGGAACTGTAGG
TCAATGGCATACTCATACTAGAACTAGAGAAATACAAGCTGTAACTAATATAGTTTCACAAAAGGCATATGTAGATATAA
ATAGAAAAGATGCAGAAAGACTTGATATAAAAGAAAATGATGAAGTTTTAATTCATTCATCAAATGGGCATACATCTAAG
TTTATAGCAAGACTAACTGATAATCTTAAAGAAAAAGATTTATATGCACCAATACACTATATAGAAACAAATTTATTAAC
ACCATCTGTATTTGATCCTTATTCTAAGGAGCCTTCATATAAAACAGTTCAAGTTAATATAGAAAAGGTTAGAAAATAG

Upstream 100 bases:

>100_bases
CGTGATAATTATCATATTATTATGGATAATATAATGATATAATTCAAATAAGTATGATAAATTTTTAACAAAGTTATGTT
TGTGAACGGAGGAAAAAAGT

Downstream 100 bases:

>100_bases
GAGGGGATTATTTTGAAAAGAATAAAAATCAATAGAGATAAATGCGTGGGATGCTTAACATGCACAACTGCTTGTATAGT
TTCTCATGATTCAGAGGATA

Product: nitrate reductase, catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCIKGLNLDKQCTKFNGSKKPLLKMKDGERKAIEWKEAF
DLFASKMTAIQEKYGKESVAYISTGQLPTEEMALLGHVGRSYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLEL
SDTIFFIGANPVIAHPIAWGRVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLALFYTLANVLIEKGWIDQDYINNY
TEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKIIHEGKRVSFWWTMGVNQSYEAVRTAQAIINLALITGNMGREGTG
ANSLTGQCNAMGSRMFSNTTALYGGGEYNNKERRKVVADILGMDENMLPSKPTLDYEQIIKGINKEEIKGLWVICTNPRH
SFSNNEEFKKAMKNLDFFVVQDIYEDTDSSKECDLYLPSVPAIKKEGFLINTERRLSAMVPVLEKEEDELSDYEIFLGIG
EALGMGSLLDKWRTPKDAFNLLRECSKGMPCDITGVTYERLRGSKGIQWPCREGEELEADERRLFEDGKYYTPSGKAKFI
FEDVTENPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQAVTNIVSQKAYVDINRKDAERLDIKENDEVLIHSSNGHTSK
FIARLTDNLKEKDLYAPIHYIETNLLTPSVFDPYSKEPSYKTVQVNIEKVRK

Sequences:

>Translated_692_residues
MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCIKGLNLDKQCTKFNGSKKPLLKMKDGERKAIEWKEAF
DLFASKMTAIQEKYGKESVAYISTGQLPTEEMALLGHVGRSYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLEL
SDTIFFIGANPVIAHPIAWGRVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLALFYTLANVLIEKGWIDQDYINNY
TEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKIIHEGKRVSFWWTMGVNQSYEAVRTAQAIINLALITGNMGREGTG
ANSLTGQCNAMGSRMFSNTTALYGGGEYNNKERRKVVADILGMDENMLPSKPTLDYEQIIKGINKEEIKGLWVICTNPRH
SFSNNEEFKKAMKNLDFFVVQDIYEDTDSSKECDLYLPSVPAIKKEGFLINTERRLSAMVPVLEKEEDELSDYEIFLGIG
EALGMGSLLDKWRTPKDAFNLLRECSKGMPCDITGVTYERLRGSKGIQWPCREGEELEADERRLFEDGKYYTPSGKAKFI
FEDVTENPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQAVTNIVSQKAYVDINRKDAERLDIKENDEVLIHSSNGHTSK
FIARLTDNLKEKDLYAPIHYIETNLLTPSVFDPYSKEPSYKTVQVNIEKVRK
>Mature_692_residues
MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCIKGLNLDKQCTKFNGSKKPLLKMKDGERKAIEWKEAF
DLFASKMTAIQEKYGKESVAYISTGQLPTEEMALLGHVGRSYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLEL
SDTIFFIGANPVIAHPIAWGRVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLALFYTLANVLIEKGWIDQDYINNY
TEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKIIHEGKRVSFWWTMGVNQSYEAVRTAQAIINLALITGNMGREGTG
ANSLTGQCNAMGSRMFSNTTALYGGGEYNNKERRKVVADILGMDENMLPSKPTLDYEQIIKGINKEEIKGLWVICTNPRH
SFSNNEEFKKAMKNLDFFVVQDIYEDTDSSKECDLYLPSVPAIKKEGFLINTERRLSAMVPVLEKEEDELSDYEIFLGIG
EALGMGSLLDKWRTPKDAFNLLRECSKGMPCDITGVTYERLRGSKGIQWPCREGEELEADERRLFEDGKYYTPSGKAKFI
FEDVTENPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQAVTNIVSQKAYVDINRKDAERLDIKENDEVLIHSSNGHTSK
FIARLTDNLKEKDLYAPIHYIETNLLTPSVFDPYSKEPSYKTVQVNIEKVRK

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=705, Percent_Identity=27.8014184397163, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1788534, Length=799, Percent_Identity=26.9086357947434, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI3868719, Length=410, Percent_Identity=24.1463414634146, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1787778, Length=294, Percent_Identity=25.5102040816327, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI3868720, Length=410, Percent_Identity=24.8780487804878, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1787870, Length=357, Percent_Identity=24.9299719887955, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081797, Length=507, Percent_Identity=23.0769230769231, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693196, Length=451, Percent_Identity=23.9467849223947, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI171474008, Length=361, Percent_Identity=24.0997229916898, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081994, Length=456, Percent_Identity=20.8333333333333, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 78531; Mature: 78531

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCIKGLNLDKQCTKFNGSK
CCHHHHHHCEEEEEEECEEEECCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHCCCCC
KPLLKMKDGERKAIEWKEAFDLFASKMTAIQEKYGKESVAYISTGQLPTEEMALLGHVGR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
SYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLELSDTIFFIGANPVIAHPIAWG
CEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEECCEECCEEEEEECCCCEECCCCCC
RVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLALFYTLANVLIEKGWIDQDYINNY
HHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
TEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKIIHEGKRVSFWWTMGVNQSYEAVRT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
AQAIINLALITGNMGREGTGANSLTGQCNAMGSRMFSNTTALYGGGEYNNKERRKVVADI
HHHHHEEHEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
LGMDENMLPSKPTLDYEQIIKGINKEEIKGLWVICTNPRHSFSNNEEFKKAMKNLDFFVV
HCCCCCCCCCCCCCCHHHHHCCCCHHHCCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEE
QDIYEDTDSSKECDLYLPSVPAIKKEGFLINTERRLSAMVPVLEKEEDELSDYEIFLGIG
HHHHHCCCCCCCCEEECCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEEECH
EALGMGSLLDKWRTPKDAFNLLRECSKGMPCDITGVTYERLRGSKGIQWPCREGEELEAD
HHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCCCCCCCCCCCCCHH
ERRLFEDGKYYTPSGKAKFIFEDVTENPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQA
HHHHHHCCCEECCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
VTNIVSQKAYVDINRKDAERLDIKENDEVLIHSSNGHTSKFIARLTDNLKEKDLYAPIHY
HHHHHHCHHEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHCCHHH
IETNLLTPSVFDPYSKEPSYKTVQVNIEKVRK
HHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCC
>Mature Secondary Structure
MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCIKGLNLDKQCTKFNGSK
CCHHHHHHCEEEEEEECEEEECCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHCCCCC
KPLLKMKDGERKAIEWKEAFDLFASKMTAIQEKYGKESVAYISTGQLPTEEMALLGHVGR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
SYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLELSDTIFFIGANPVIAHPIAWG
CEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEECCEECCEEEEEECCCCEECCCCCC
RVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLALFYTLANVLIEKGWIDQDYINNY
HHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
TEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKIIHEGKRVSFWWTMGVNQSYEAVRT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
AQAIINLALITGNMGREGTGANSLTGQCNAMGSRMFSNTTALYGGGEYNNKERRKVVADI
HHHHHEEHEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
LGMDENMLPSKPTLDYEQIIKGINKEEIKGLWVICTNPRHSFSNNEEFKKAMKNLDFFVV
HCCCCCCCCCCCCCCHHHHHCCCCHHHCCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEE
QDIYEDTDSSKECDLYLPSVPAIKKEGFLINTERRLSAMVPVLEKEEDELSDYEIFLGIG
HHHHHCCCCCCCCEEECCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEEECH
EALGMGSLLDKWRTPKDAFNLLRECSKGMPCDITGVTYERLRGSKGIQWPCREGEELEAD
HHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCCCCCCCCCCCCCHH
ERRLFEDGKYYTPSGKAKFIFEDVTENPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQA
HHHHHHCCCEECCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
VTNIVSQKAYVDINRKDAERLDIKENDEVLIHSSNGHTSKFIARLTDNLKEKDLYAPIHY
HHHHHHCHHEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHCCHHH
IETNLLTPSVFDPYSKEPSYKTVQVNIEKVRK
HHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]