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The map label for this gene is nadB [C]

Identifier: 18310413

GI number: 18310413

Start: 1687324

End: 1688949

Strand: Reverse

Name: nadB [C]

Synonym: CPE1431

Alternate gene names: 18310413

Gene position: 1688949-1687324 (Counterclockwise)

Preceding gene: 18310414

Following gene: 18310410

Centisome position: 55.71

GC content: 29.34

Gene sequence:

>1626_bases
ATGAGAGAAAAAGTGAAGTTAAAAAAAGATAAAGAATGTGATGTTTTTGTAATGGGAGCTGGAATTGCAGGAATTATGGC
AGCAATTGAAGCCTCTAATAATGGTCAAAAGGTAATAATCTCAAGTAGTTCAAATATCTTTTCAGGGTCAAGCTTTTATC
CTGGTACATGGGGCTTAGGTTTAATAGGTCCAGAGGATAGAAATGATGAAGAAGATTTAGCTAAGACAATTAAAGAAGTT
GGGTGCAATGTTGTTGATGAGGAATTAGTAGAAACCTTTGTTAAAAATATTAATCCAAGTATAGATTTATTAAAAGAAAT
GGGTGTTAAGCTTAAAGAAGCTAATAATTCAGGAGAAAAAGAATTTATTCCATGTTTTGACCATAAAAATAGAAATTGGA
ATGGTATTTTATTTGAAAGTGCAAAAAAAGTTTTAGACCATAAGTTAAAGAGCAATGAAGTTTTGGAATATCCTTTTTCA
GAGGTAATTGAAATAGTAAAGGAAGATAATAAGATTATAGGTGTAATACTTATTAACAGTTTAAATAAGCTTGAGTTTAT
AAAATGTAAAGCATTTGTAATTGCCTCTGGAGGAATAGGTGGTTTATTTAAATATAGGTTAAATACTAAAGATATAACAG
GTATGGGACAAGCTTTAGCCTTAAAGGTTGGGTGTAACTTAACAAATATAGAGTTTATGCAAATGATGCCAGGATATATA
AAGCCCTGTCAAAAAACTATATTTAATGAGAAAACCTTTAAATATATAGAAGCTAGAAATGAAGAGGGAGAAGATGTATT
TAAAGATATAGAAAATCTAAGAGAAAAGCTTGAAAAAAGATCAACTTATGGACCTTTTACTTCAAGATTAGACTCTAAGG
ATATTGACTATGCTATATTTAAAGAGTTTATGAAAAATAAAAATGGAGTTACTGTTAGATATAAAGATAGTTTAAAGAAT
AATATGCCAGAGTTTATTGAAGTTTATTTTAAATGGTTAAAGGAAAATAAACATCTTACCCCAGATGATGAAATAAATAT
TGGAATATTCTTTCATGCAGCCAATGGAGGAATTAGTATAAATAAAAAAGCAGAAACAAAGGTAGATGGATTATTTGCAG
CTGGAGAATGTACAGGTGGTATGCATGGTGCAGACAGAATAGGGGGATTATCTACAGCAAATGGTCTTGTTTTTGGTAGA
ATAGCTGGAGAAAGTGCTAGTAGATATGCTTCTAGTAAAGGTTTAAGTGAAAAAGAAGAAGTAGAATTTGAAGCTTATGA
AATAGAGGGAGCAGGGGATCTTATTTTAGAAGTTCAAGAAATTATGTTTAAAAATGCAATGATAGAAAAAAGTGAAGTAG
GTGTTAAAGATTCACTTTTAAAATTAGAGGAAATATCTAAGGGGTTTGTATATAAGAAAGAAGTAAATATGGAGAATTTA
AGAAATTCATATAGACTAGAAAACAATATATTGTTATGCAAGGCCATATTAAAAGCAATTGATCTTAGAAAAGAAAGTAG
AGGTTCTCACTATAGATATGATTATCCAAATTGTGATAAAAACATGGATAAGATGATAATGGTAGAGTTAGGTGATGATA
TTAAAGCTTATTTTAAAGAAAATTAG

Upstream 100 bases:

>100_bases
ATGTTTTTGTATTAGAAATTTTCTTCTTAATTAAGATATTTATATGCCTATAAATCAGCTTTAATTTTATAATTGGAAAT
TTTAATTAGGTGGGGTATTT

Downstream 100 bases:

>100_bases
AATTACTATAAATAATTAAATTCTAATAGATTATTAAGATAGCCTTTTATATCTAGAAATCAATATTATTTTAAAATAGT
GGTTAATTAATATAATTTAA

Product: FAD flavoprotein oxidase

Products: oxaloacetate; NH3; H2O2; iminoaspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MREKVKLKKDKECDVFVMGAGIAGIMAAIEASNNGQKVIISSSSNIFSGSSFYPGTWGLGLIGPEDRNDEEDLAKTIKEV
GCNVVDEELVETFVKNINPSIDLLKEMGVKLKEANNSGEKEFIPCFDHKNRNWNGILFESAKKVLDHKLKSNEVLEYPFS
EVIEIVKEDNKIIGVILINSLNKLEFIKCKAFVIASGGIGGLFKYRLNTKDITGMGQALALKVGCNLTNIEFMQMMPGYI
KPCQKTIFNEKTFKYIEARNEEGEDVFKDIENLREKLEKRSTYGPFTSRLDSKDIDYAIFKEFMKNKNGVTVRYKDSLKN
NMPEFIEVYFKWLKENKHLTPDDEINIGIFFHAANGGISINKKAETKVDGLFAAGECTGGMHGADRIGGLSTANGLVFGR
IAGESASRYASSKGLSEKEEVEFEAYEIEGAGDLILEVQEIMFKNAMIEKSEVGVKDSLLKLEEISKGFVYKKEVNMENL
RNSYRLENNILLCKAILKAIDLRKESRGSHYRYDYPNCDKNMDKMIMVELGDDIKAYFKEN

Sequences:

>Translated_541_residues
MREKVKLKKDKECDVFVMGAGIAGIMAAIEASNNGQKVIISSSSNIFSGSSFYPGTWGLGLIGPEDRNDEEDLAKTIKEV
GCNVVDEELVETFVKNINPSIDLLKEMGVKLKEANNSGEKEFIPCFDHKNRNWNGILFESAKKVLDHKLKSNEVLEYPFS
EVIEIVKEDNKIIGVILINSLNKLEFIKCKAFVIASGGIGGLFKYRLNTKDITGMGQALALKVGCNLTNIEFMQMMPGYI
KPCQKTIFNEKTFKYIEARNEEGEDVFKDIENLREKLEKRSTYGPFTSRLDSKDIDYAIFKEFMKNKNGVTVRYKDSLKN
NMPEFIEVYFKWLKENKHLTPDDEINIGIFFHAANGGISINKKAETKVDGLFAAGECTGGMHGADRIGGLSTANGLVFGR
IAGESASRYASSKGLSEKEEVEFEAYEIEGAGDLILEVQEIMFKNAMIEKSEVGVKDSLLKLEEISKGFVYKKEVNMENL
RNSYRLENNILLCKAILKAIDLRKESRGSHYRYDYPNCDKNMDKMIMVELGDDIKAYFKEN
>Mature_541_residues
MREKVKLKKDKECDVFVMGAGIAGIMAAIEASNNGQKVIISSSSNIFSGSSFYPGTWGLGLIGPEDRNDEEDLAKTIKEV
GCNVVDEELVETFVKNINPSIDLLKEMGVKLKEANNSGEKEFIPCFDHKNRNWNGILFESAKKVLDHKLKSNEVLEYPFS
EVIEIVKEDNKIIGVILINSLNKLEFIKCKAFVIASGGIGGLFKYRLNTKDITGMGQALALKVGCNLTNIEFMQMMPGYI
KPCQKTIFNEKTFKYIEARNEEGEDVFKDIENLREKLEKRSTYGPFTSRLDSKDIDYAIFKEFMKNKNGVTVRYKDSLKN
NMPEFIEVYFKWLKENKHLTPDDEINIGIFFHAANGGISINKKAETKVDGLFAAGECTGGMHGADRIGGLSTANGLVFGR
IAGESASRYASSKGLSEKEEVEFEAYEIEGAGDLILEVQEIMFKNAMIEKSEVGVKDSLLKLEEISKGFVYKKEVNMENL
RNSYRLENNILLCKAILKAIDLRKESRGSHYRYDYPNCDKNMDKMIMVELGDDIKAYFKEN

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790597, Length=425, Percent_Identity=25.4117647058824, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.4.3.16 [C]

Molecular weight: Translated: 60946; Mature: 60946

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKVKLKKDKECDVFVMGAGIAGIMAAIEASNNGQKVIISSSSNIFSGSSFYPGTWGLG
CCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
LIGPEDRNDEEDLAKTIKEVGCNVVDEELVETFVKNINPSIDLLKEMGVKLKEANNSGEK
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCC
EFIPCFDHKNRNWNGILFESAKKVLDHKLKSNEVLEYPFSEVIEIVKEDNKIIGVILINS
CEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCCEEEEEEECC
LNKLEFIKCKAFVIASGGIGGLFKYRLNTKDITGMGQALALKVGCNLTNIEFMQMMPGYI
CCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHCCHHH
KPCQKTIFNEKTFKYIEARNEEGEDVFKDIENLREKLEKRSTYGPFTSRLDSKDIDYAIF
HHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHH
KEFMKNKNGVTVRYKDSLKNNMPEFIEVYFKWLKENKHLTPDDEINIGIFFHAANGGISI
HHHHCCCCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCEEE
NKKAETKVDGLFAAGECTGGMHGADRIGGLSTANGLVFGRIAGESASRYASSKGLSEKEE
CCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHC
VEFEAYEIEGAGDLILEVQEIMFKNAMIEKSEVGVKDSLLKLEEISKGFVYKKEVNMENL
CEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCEEEECCCHHHH
RNSYRLENNILLCKAILKAIDLRKESRGSHYRYDYPNCDKNMDKMIMVELGDDIKAYFKE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCHHHHHHCC
N
C
>Mature Secondary Structure
MREKVKLKKDKECDVFVMGAGIAGIMAAIEASNNGQKVIISSSSNIFSGSSFYPGTWGLG
CCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
LIGPEDRNDEEDLAKTIKEVGCNVVDEELVETFVKNINPSIDLLKEMGVKLKEANNSGEK
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCC
EFIPCFDHKNRNWNGILFESAKKVLDHKLKSNEVLEYPFSEVIEIVKEDNKIIGVILINS
CEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCCEEEEEEECC
LNKLEFIKCKAFVIASGGIGGLFKYRLNTKDITGMGQALALKVGCNLTNIEFMQMMPGYI
CCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHCCHHH
KPCQKTIFNEKTFKYIEARNEEGEDVFKDIENLREKLEKRSTYGPFTSRLDSKDIDYAIF
HHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHH
KEFMKNKNGVTVRYKDSLKNNMPEFIEVYFKWLKENKHLTPDDEINIGIFFHAANGGISI
HHHHCCCCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCEEE
NKKAETKVDGLFAAGECTGGMHGADRIGGLSTANGLVFGRIAGESASRYASSKGLSEKEE
CCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHC
VEFEAYEIEGAGDLILEVQEIMFKNAMIEKSEVGVKDSLLKLEEISKGFVYKKEVNMENL
CEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCEEEECCCHHHH
RNSYRLENNILLCKAILKAIDLRKESRGSHYRYDYPNCDKNMDKMIMVELGDDIKAYFKE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: L-Aspartate [C]

Kcat value (1/min): 2 [C]

Specific activity: NA

Km value (mM): 4.1 {L-aspartate}} 0.5 {L-aspartate}} [C]

Substrates: @ASPA02txt*L-Aspartate!; H2O; O2; L-aspartate [C]

Specific reaction: @ASOA02.txt*L-Aspartate! + H2O + O2 = oxaloacetate + NH3 + H2O2 O2 + L-aspartate = H2O2 + iminoaspartate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: Iodoacetate; L-Aspartate; Mesotartrate; NAD+; Tetranitromethane; Urea [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]