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The map label for this gene is hemL [H]

Identifier: 18310414

GI number: 18310414

Start: 1689150

End: 1690433

Strand: Reverse

Name: hemL [H]

Synonym: CPE1432

Alternate gene names: 18310414

Gene position: 1690433-1689150 (Counterclockwise)

Preceding gene: 18310415

Following gene: 18310413

Centisome position: 55.76

GC content: 32.55

Gene sequence:

>1284_bases
ATGAATATGGACAGAAATAAAGAAATATTTGAAGAATCAAAAAAATATATGCCAGGTGGGGTTAATAGTCCTGTAAGATC
TTTTGGAAGTGTTGGAATAAATCCTCCAGTAATAAAAAGTGGTAAAGGAGCTATGATAAAGGACGAGAATGGAAATGAAT
ATATTGACTTTGTTTTAGCTTGGGGACCAATGATATTAGGTCATTGTGACGAAGATGTAGTAGAAGCAATAAAGAAAACT
TCAGAGGAAAGTATAGCCTTTGGTGCTTCAACAAAATTAGAATTAGACTTAGCTAAGCTTTTATGTGAAACATTAGACAA
TGTCGACATGATAAGAATGGTAAACTCAGGTACAGAGGCTACTATGAGTGCTGTAAAGTTAGCAAGAGGATACACAAAGA
AAGATAAAATAATTAAGTTTGCAGGATGCTATCATGGACACTTTGATGGATTCTTAATAGAAGCTGGATCAGGAGTATTA
ACAGAAGGAATTCCAGGATGCTTAGGTGTTCCAGAGGAAAGTATAAAGAATACTTTAATAGGAATATACAATGATGAGAA
GCAAGTTGAAGAATTATTTGAAAAGTACGGAAATGATATTGCTGGAATAATAATAGAGCCAGTTGCTGGAAATATGGGTG
TTGTTAAATGTGATCCTAAGTTTATGAGAAAACTTAGAGAATTATGTGACAAATATGGAGCACTTCTTATATTTGATGAA
GTTATGTGTGGATTTAGAGTAGCATATAAAGGAGCACAAACTTTATTTGATGTTAAGCCAGATTTAGTTACTTATGCTAA
AATAATGGGTGGTGGACTACCTTGTGGAGCATATGGTGGAAGAAGAGAGATAATGGAAAACTTATCACCACTAGGTGGAG
TTTACCAAGCTGGAACAATGTCAGGAAATCCAATAGTAATGAGTGCAGGACTTGCAACAGTTAAAAAACTTTATGAAAAT
CCATCATACTATAATCATATTGAAAAAATAGGATCAAAACTTGAAAAGGGAGTTCTTGAAATAGCAAAGAAAAAAGGTTT
AGGTCTTGTTGTTAATAGACAAGGTGGCATGATAACTTTATTCTTTACTGATTTAAAAGAAGTTAAGTGTTATGATGATG
TAAAAACTTGTGATGGAGAAAGATTTAAGAGATATTTCTTACATATGTTAAACAAAGGATTTAACATACCACCATCACAA
TTTGAGGCAATGTTCTTAAGTGTAAAACATACAGAAGAACATATAGATAAATTCTTAGAAGCTTTTGAAACCTTTGAAGG
ATAA

Upstream 100 bases:

>100_bases
AACTTATTATGCTATAGAAGCTGCTAAATGGTTACAAGAATAGTTTAAGACTATAATATAATGAAAAAAATATTTGCTTA
ACAATCTTATAGTTAGGAGA

Downstream 100 bases:

>100_bases
TAAAATTTAAAAAGTTTTAAATGAATGCTGATTTTTAGGTATATATTTAAGAAAAGACTTAATTTAATAGGAAGCTTTAT
TACAAATTTAATATACTTAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGAMIKDENGNEYIDFVLAWGPMILGHCDEDVVEAIKKT
SEESIAFGASTKLELDLAKLLCETLDNVDMIRMVNSGTEATMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVL
TEGIPGCLGVPEESIKNTLIGIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCDPKFMRKLRELCDKYGALLIFDE
VMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCGAYGGRREIMENLSPLGGVYQAGTMSGNPIVMSAGLATVKKLYEN
PSYYNHIEKIGSKLEKGVLEIAKKKGLGLVVNRQGGMITLFFTDLKEVKCYDDVKTCDGERFKRYFLHMLNKGFNIPPSQ
FEAMFLSVKHTEEHIDKFLEAFETFEG

Sequences:

>Translated_427_residues
MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGAMIKDENGNEYIDFVLAWGPMILGHCDEDVVEAIKKT
SEESIAFGASTKLELDLAKLLCETLDNVDMIRMVNSGTEATMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVL
TEGIPGCLGVPEESIKNTLIGIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCDPKFMRKLRELCDKYGALLIFDE
VMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCGAYGGRREIMENLSPLGGVYQAGTMSGNPIVMSAGLATVKKLYEN
PSYYNHIEKIGSKLEKGVLEIAKKKGLGLVVNRQGGMITLFFTDLKEVKCYDDVKTCDGERFKRYFLHMLNKGFNIPPSQ
FEAMFLSVKHTEEHIDKFLEAFETFEG
>Mature_427_residues
MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGAMIKDENGNEYIDFVLAWGPMILGHCDEDVVEAIKKT
SEESIAFGASTKLELDLAKLLCETLDNVDMIRMVNSGTEATMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVL
TEGIPGCLGVPEESIKNTLIGIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCDPKFMRKLRELCDKYGALLIFDE
VMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCGAYGGRREIMENLSPLGGVYQAGTMSGNPIVMSAGLATVKKLYEN
PSYYNHIEKIGSKLEKGVLEIAKKKGLGLVVNRQGGMITLFFTDLKEVKCYDDVKTCDGERFKRYFLHMLNKGFNIPPSQ
FEAMFLSVKHTEEHIDKFLEAFETFEG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=363, Percent_Identity=28.099173553719, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI13994255, Length=322, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI24119277, Length=321, Percent_Identity=25.8566978193146, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI37574042, Length=322, Percent_Identity=27.3291925465839, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI284507298, Length=278, Percent_Identity=28.4172661870504, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI226442705, Length=323, Percent_Identity=25.6965944272446, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI226442709, Length=290, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=50.5966587112172, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1789016, Length=377, Percent_Identity=31.8302387267904, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1789759, Length=330, Percent_Identity=29.3939393939394, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788044, Length=304, Percent_Identity=30.9210526315789, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693181, Length=356, Percent_Identity=29.2134831460674, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787560, Length=344, Percent_Identity=28.7790697674419, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1786991, Length=326, Percent_Identity=25.1533742331288, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71992977, Length=352, Percent_Identity=29.8295454545455, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=348, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=332, Percent_Identity=24.6987951807229, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=366, Percent_Identity=26.5027322404372, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324432, Length=326, Percent_Identity=27.6073619631902, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357415, Length=349, Percent_Identity=27.2206303724928, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 47127; Mature: 47127

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGAMIKDENGNEYIDFVLA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEECCCCCEEECCCCCHHHHHHHH
WGPMILGHCDEDVVEAIKKTSEESIAFGASTKLELDLAKLLCETLDNVDMIRMVNSGTEA
HHHHHHCCCCHHHHHHHHHCCHHHEEECCCCEEHHHHHHHHHHHHCCCCEEHHHCCCHHH
TMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVLTEGIPGCLGVPEESIKNTLI
HHHHHHHHHCCCCHHCEEEEECEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCEE
GIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCDPKFMRKLRELCDKYGALLIFDE
EEECCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCEEEHHH
VMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCGAYGGRREIMENLSPLGGVYQAGTM
HHHHHHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCC
SGNPIVMSAGLATVKKLYENPSYYNHIEKIGSKLEKGVLEIAKKKGLGLVVNRQGGMITL
CCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
FFTDLKEVKCYDDVKTCDGERFKRYFLHMLNKGFNIPPSQFEAMFLSVKHTEEHIDKFLE
EECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFETFEG
HHHHCCC
>Mature Secondary Structure
MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGAMIKDENGNEYIDFVLA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEECCCCCEEECCCCCHHHHHHHH
WGPMILGHCDEDVVEAIKKTSEESIAFGASTKLELDLAKLLCETLDNVDMIRMVNSGTEA
HHHHHHCCCCHHHHHHHHHCCHHHEEECCCCEEHHHHHHHHHHHHCCCCEEHHHCCCHHH
TMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVLTEGIPGCLGVPEESIKNTLI
HHHHHHHHHCCCCHHCEEEEECEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCEE
GIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCDPKFMRKLRELCDKYGALLIFDE
EEECCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCEEEHHH
VMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCGAYGGRREIMENLSPLGGVYQAGTM
HHHHHHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCC
SGNPIVMSAGLATVKKLYENPSYYNHIEKIGSKLEKGVLEIAKKKGLGLVVNRQGGMITL
CCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
FFTDLKEVKCYDDVKTCDGERFKRYFLHMLNKGFNIPPSQFEAMFLSVKHTEEHIDKFLE
EECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFETFEG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10585141 [H]