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The map label for this gene is thiM [H]

Identifier: 18310319

GI number: 18310319

Start: 1579258

End: 1580055

Strand: Reverse

Name: thiM [H]

Synonym: CPE1337

Alternate gene names: 18310319

Gene position: 1580055-1579258 (Counterclockwise)

Preceding gene: 18310320

Following gene: 18310318

Centisome position: 52.12

GC content: 30.08

Gene sequence:

>798_bases
ATGGAAGTTTTGAAAAGAGAAAATCCTTTAATACATATGATAACTAATTATGTTACAGTTAATGATTTAGCACAAGTAAC
TATTAATTATGGCGGCTTACCTCTTATGGCTACCCATCATGATGAATTAAAAGAGATAACAAAAATGGCTAATGGATTAT
TAGTCAATATAGGAACTCTAGAACCTTACCAAATGGAGTCGTCAATGATTTCTATGAAAATAGCAAAAGAAAAAGGAATT
CCATCTGTTTTAGATCCAGTAGGTGTACAAGCATCCAAACTTAGAAGAGATTTTGCTAAAAAAATAATACTAGAGGGAGA
GCCTTCACTTATAAAGGGGAATTTAGCTGAAATAAAAACCCTTATAGGTGAAACTTCAAATTCAATTGGAATAGATTCCT
TTGAGGATTCTTTAAGTGAAAATACAAAAAATAAGATAAAGGAATATGCAAGGGAAAGAAATCTTGTAGTAGTTGTTTCT
GGAGTGGTTGATTTTATAACTAATGGAGAAGAAAGTGCAAGTGTTAAAAATGGAACTTATAAAATGAGTAAAATTACAGG
TACTGGATGTATGTTAGGAGCTTTATTAACACTAGCTTTAAGTTTTTATGACCATAAAGATTTAAGATTTAAAGAAGTGG
TTAAAGCAGTATCTACATGGGGAATATGTGGAGAATTAGCTGAGGAAAGATTAAGAGAAAAAGAAGGGTTAATGACATTT
AAGCATAATCTTTTAGATGAACTTTCAATTATTAATGATGAAACAATAAAGGAAAGAGAGAAGGTTATTTATGAGTAA

Upstream 100 bases:

>100_bases
GAAAAGAATATATTACAGGTGCTATAAAAAATGGCTTTGAACTTGGAAAGGGTGTTGGACCAACACATCATTTCTATAAA
TTTTACTAGGAGGAATAAAA

Downstream 100 bases:

>100_bases
TAAAGATTACAAAAAATTATATTTAGTAACAGACTATAGAATTCCCTTTAATGAGCTTTTAGAAAAAACAAAGGAAGCTT
TAATTGGAGGGGTATCCATT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELKEITKMANGLLVNIGTLEPYQMESSMISMKIAKEKGI
PSVLDPVGVQASKLRRDFAKKIILEGEPSLIKGNLAEIKTLIGETSNSIGIDSFEDSLSENTKNKIKEYARERNLVVVVS
GVVDFITNGEESASVKNGTYKMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVSTWGICGELAEERLREKEGLMTF
KHNLLDELSIINDETIKEREKVIYE

Sequences:

>Translated_265_residues
MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELKEITKMANGLLVNIGTLEPYQMESSMISMKIAKEKGI
PSVLDPVGVQASKLRRDFAKKIILEGEPSLIKGNLAEIKTLIGETSNSIGIDSFEDSLSENTKNKIKEYARERNLVVVVS
GVVDFITNGEESASVKNGTYKMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVSTWGICGELAEERLREKEGLMTF
KHNLLDELSIINDETIKEREKVIYE
>Mature_265_residues
MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELKEITKMANGLLVNIGTLEPYQMESSMISMKIAKEKGI
PSVLDPVGVQASKLRRDFAKKIILEGEPSLIKGNLAEIKTLIGETSNSIGIDSFEDSLSENTKNKIKEYARERNLVVVVS
GVVDFITNGEESASVKNGTYKMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVSTWGICGELAEERLREKEGLMTF
KHNLLDELSIINDETIKEREKVIYE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=29.1338582677165, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325042, Length=274, Percent_Identity=27.7372262773723, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 29538; Mature: 29538

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELKEITKMANGLLVNIGTL
CCCCCCCCCEEEEHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHCCEEEEECCC
EPYQMESSMISMKIAKEKGIPSVLDPVGVQASKLRRDFAKKIILEGEPSLIKGNLAEIKT
CCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LIGETSNSIGIDSFEDSLSENTKNKIKEYARERNLVVVVSGVVDFITNGEESASVKNGTY
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCCE
KMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVSTWGICGELAEERLREKEGLMTF
EEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KHNLLDELSIINDETIKEREKVIYE
HHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELKEITKMANGLLVNIGTL
CCCCCCCCCEEEEHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHCCEEEEECCC
EPYQMESSMISMKIAKEKGIPSVLDPVGVQASKLRRDFAKKIILEGEPSLIKGNLAEIKT
CCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LIGETSNSIGIDSFEDSLSENTKNKIKEYARERNLVVVVSGVVDFITNGEESASVKNGTY
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCCE
KMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVSTWGICGELAEERLREKEGLMTF
EEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KHNLLDELSIINDETIKEREKVIYE
HHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA