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The map label for this gene is thiE [H]

Identifier: 18310318

GI number: 18310318

Start: 1578642

End: 1579265

Strand: Reverse

Name: thiE [H]

Synonym: CPE1336

Alternate gene names: 18310318

Gene position: 1579265-1578642 (Counterclockwise)

Preceding gene: 18310319

Following gene: 18310317

Centisome position: 52.1

GC content: 29.33

Gene sequence:

>624_bases
ATGAGTAATAAAGATTACAAAAAATTATATTTAGTAACAGACTATAGAATTCCCTTTAATGAGCTTTTAGAAAAAACAAA
GGAAGCTTTAATTGGAGGGGTATCCATTGTTCAATATAGAGCAAAGAATAAAAAAACTAAAGAAATGTGTAAGGAAGCTA
AGGAGTTAAAAAAGCTTTGTGATGAGTTTGGAGCATTATTTTTAGTAAATGATAGAATAGATGTAGCCTTAGCAGTAAAA
GCTAATGGAGTTCATATAGGACAAGATGATATGGAAGTTTCCATAGCAAGAGAAATAATGCCAAAGGATGCAGTTATAGG
TGTTACTGTTCATAATAAAGAGGAAGCTTTAAAAGCTATAAAAGAGGGAGCAGATAACTTAGGTGTTGGAGCTTTATTTT
CAACTAATAGTAAAGATGATGCAACATTAATGACCTTAGAAACCTTAAGAGAGATAAAATCTGTTTCTAATATTCCACTT
TATGGAATTGGAGGTATAACTCCTTATAACTTAAATAAGGATATTTTAGAAAATCTAGATGGGGTAGCTGTCATATCTGC
TTTGTTAAATAGTGATAATATAAGAGAAAAAAGCAAGGAATTTTTAAATATTCTTAGTAAATAA

Upstream 100 bases:

>100_bases
TTAAGAGAAAAAGAAGGGTTAATGACATTTAAGCATAATCTTTTAGATGAACTTTCAATTATTAATGATGAAACAATAAA
GGAAAGAGAGAAGGTTATTT

Downstream 100 bases:

>100_bases
AAATTTTTGAATATTAAACTTAAAATATGAAATAATCAAAAAGAGGTTCTTAAGTTAGAGAATCTCTTTTTTATTTAGAG
TTTATTTTATAGTATAATAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNKKTKEMCKEAKELKKLCDEFGALFLVNDRIDVALAVK
ANGVHIGQDDMEVSIAREIMPKDAVIGVTVHNKEEALKAIKEGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPL
YGIGGITPYNLNKDILENLDGVAVISALLNSDNIREKSKEFLNILSK

Sequences:

>Translated_207_residues
MSNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNKKTKEMCKEAKELKKLCDEFGALFLVNDRIDVALAVK
ANGVHIGQDDMEVSIAREIMPKDAVIGVTVHNKEEALKAIKEGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPL
YGIGGITPYNLNKDILENLDGVAVISALLNSDNIREKSKEFLNILSK
>Mature_206_residues
SNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNKKTKEMCKEAKELKKLCDEFGALFLVNDRIDVALAVKA
NGVHIGQDDMEVSIAREIMPKDAVIGVTVHNKEEALKAIKEGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPLY
GIGGITPYNLNKDILENLDGVAVISALLNSDNIREKSKEFLNILSK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=189, Percent_Identity=24.8677248677249, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=223, Percent_Identity=31.8385650224215, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22919; Mature: 22788

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNKKTKEMCKEAKELKKLC
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DEFGALFLVNDRIDVALAVKANGVHIGQDDMEVSIAREIMPKDAVIGVTVHNKEEALKAI
HHHCCEEEEECCEEEEEEEEECCEEECCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
KEGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPLYGIGGITPYNLNKDILENLD
HCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCC
GVAVISALLNSDNIREKSKEFLNILSK
HHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNKKTKEMCKEAKELKKLC
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DEFGALFLVNDRIDVALAVKANGVHIGQDDMEVSIAREIMPKDAVIGVTVHNKEEALKAI
HHHCCEEEEECCEEEEEEEEECCEEECCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
KEGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPLYGIGGITPYNLNKDILENLD
HCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCC
GVAVISALLNSDNIREKSKEFLNILSK
HHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA