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The map label for this gene is pyrB [H]

Identifier: 18310165

GI number: 18310165

Start: 1378381

End: 1379304

Strand: Reverse

Name: pyrB [H]

Synonym: CPE1183

Alternate gene names: 18310165

Gene position: 1379304-1378381 (Counterclockwise)

Preceding gene: 18310166

Following gene: 18310164

Centisome position: 45.5

GC content: 31.06

Gene sequence:

>924_bases
ATGTTAAAAAACAAACACTTATTAGATCCATCAGATTTCACAATAGAGGAATTTGATGAAATTTTCAAATTAGCACATCA
AATTATGGCAAATCCAAAAGAATATCAAAACATATGCAATGGTAAAATTCTAGCAACACTTTTCTATGAGCCAAGCACTA
GAACAAGATTATCTTTTGAATCAGCAATGCTTAGACTAGGTGGACAAGTTATAGGTTTCTCAGAACCAAATTCTTCATCA
GTTTCTAAAGGAGAATCTTTAAGAGATACTATTAAAACAGTAAACTGCTATGCAGATCTTATAGCAATGAGACATCCTTT
AGAAGGAGCAGCAAAAGTTGCTTCAATGTATTCAGATATCCCAGTTATAAATGCAGGAGATGGAGGTCATCAACATCCAA
CTCAAACATTAACAGATCTTCTTACAATAAAAGAATATAAAGGAAACCTAGAAGGAAATACAATTGCATTATGTGGAGAC
TTAAAATTCGGTAGAACAGTGCATTCTCTTATAAAAGCACTTTCAAGATATAAAAATAATAAATTCATCTTAATATCTCC
AATAGAACTAAGAATCCCCAATTATATTAGAGAGCAAATTTTAGAAAAAAATAATATAGAATACAAAGAAATTACTAGCT
TAGAAGAAGGAATTAAGGAAGCTAACATTTTATACATGACAAGAATACAAAGAGAAAGATTTGTAGATCAAAGTGAGTAT
GAAAGATTAAAAGATGTATATGTATTAGATGAAGCAAAAATGAAGGGTGCCAAAGAAGATATGATGGTTCTTCATCCTCT
TCCAAGAGTAAATGAAATATCCTATGAAGTAGATGAAGATTCAAGAGCATTTTACTTTAAGCAAGCCAAATGCGGAATGT
ATGTAAGAATGGCCTTAATGGCAAAACTTTTAGGGGAGGCGTAA

Upstream 100 bases:

>100_bases
GTGAAAAGTCTTCCTTATCTTAGGAAGACTTTTTTTAATAAAATTATATAGCTTTTCTTCTTTAAATTAACACAGAGAGG
TTAAAAAGGAGTGAAATTTT

Downstream 100 bases:

>100_bases
TAGGATGAAAAATAGAGAATTACAAAATCATAAATGTAAAAACACAAAATGTATAACTCAAGTAGAAAAATATGTTCCTC
AAAGCTTTACTTTAGTAGAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLKNKHLLDPSDFTIEEFDEIFKLAHQIMANPKEYQNICNGKILATLFYEPSTRTRLSFESAMLRLGGQVIGFSEPNSSS
VSKGESLRDTIKTVNCYADLIAMRHPLEGAAKVASMYSDIPVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGD
LKFGRTVHSLIKALSRYKNNKFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGIKEANILYMTRIQRERFVDQSEY
ERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEISYEVDEDSRAFYFKQAKCGMYVRMALMAKLLGEA

Sequences:

>Translated_307_residues
MLKNKHLLDPSDFTIEEFDEIFKLAHQIMANPKEYQNICNGKILATLFYEPSTRTRLSFESAMLRLGGQVIGFSEPNSSS
VSKGESLRDTIKTVNCYADLIAMRHPLEGAAKVASMYSDIPVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGD
LKFGRTVHSLIKALSRYKNNKFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGIKEANILYMTRIQRERFVDQSEY
ERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEISYEVDEDSRAFYFKQAKCGMYVRMALMAKLLGEA
>Mature_307_residues
MLKNKHLLDPSDFTIEEFDEIFKLAHQIMANPKEYQNICNGKILATLFYEPSTRTRLSFESAMLRLGGQVIGFSEPNSSS
VSKGESLRDTIKTVNCYADLIAMRHPLEGAAKVASMYSDIPVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGD
LKFGRTVHSLIKALSRYKNNKFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGIKEANILYMTRIQRERFVDQSEY
ERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEISYEVDEDSRAFYFKQAKCGMYVRMALMAKLLGEA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=46.7320261437908, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI38788445, Length=322, Percent_Identity=27.9503105590062, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=46.2295081967213, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1786469, Length=339, Percent_Identity=26.8436578171091, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790703, Length=145, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=309, Percent_Identity=43.6893203883495, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=44.1176470588235, Blast_Score=269, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=45.2768729641694, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35033; Mature: 35033

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNKHLLDPSDFTIEEFDEIFKLAHQIMANPKEYQNICNGKILATLFYEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCHHHHH
SAMLRLGGQVIGFSEPNSSSVSKGESLRDTIKTVNCYADLIAMRHPLEGAAKVASMYSDI
HHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGDLKFGRTVHSLIKALSRYKNN
CEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
KFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGIKEANILYMTRIQRERFVDQSEY
CEEEEECHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
ERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEISYEVDEDSRAFYFKQAKCGMYVRMALM
HHHHHHHEECHHHHCCCCCCEEEECCCCCCCCCEEEECCCCCEEEEEHHHCCHHHHHHHH
AKLLGEA
HHHHCCC
>Mature Secondary Structure
MLKNKHLLDPSDFTIEEFDEIFKLAHQIMANPKEYQNICNGKILATLFYEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCHHHHH
SAMLRLGGQVIGFSEPNSSSVSKGESLRDTIKTVNCYADLIAMRHPLEGAAKVASMYSDI
HHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGDLKFGRTVHSLIKALSRYKNN
CEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
KFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGIKEANILYMTRIQRERFVDQSEY
CEEEEECHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
ERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEISYEVDEDSRAFYFKQAKCGMYVRMALM
HHHHHHHEECHHHHCCCCCCEEEECCCCCCCCCEEEECCCCCEEEEEHHHCCHHHHHHHH
AKLLGEA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA