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The map label for this gene is miaA [H]

Identifier: 18310139

GI number: 18310139

Start: 1355805

End: 1356737

Strand: Direct

Name: miaA [H]

Synonym: CPE1157

Alternate gene names: 18310139

Gene position: 1355805-1356737 (Clockwise)

Preceding gene: 18310138

Following gene: 18310140

Centisome position: 44.72

GC content: 29.15

Gene sequence:

>933_bases
ATGAATAATAATTTACTTATTATTGCTGGGCCAACAGCCGTAGGAAAAAGTGACCTTTCAGTAGATCTAGCAAAGAAGCT
AAATGGTGAAATAATCTCTGTTGACTCTATGCAAATATATAAATACATGGATATAGGTTCAGCTAAAATTTCTAAGGAAG
AAATGGGAGGTATTCCTCACTATTTAATAGATTTTGTTGATCCTAGTAAGGAATTTAGTGTAGCTGAATTTAAAGACTTA
ACTACAGAGAAAATTAAAGATATACAAAGTAGAGGGAAACTTCCTATATTAGTAGGGGGCACTGGACTATATATAAATTC
AATCATATGCAATATGAATTTTGCAGAAAGTGATAAGGATGAAGAGTATAGAGAAGAGCTTGAAAAAATAGCTAATGAGC
ATGGAAATGAATATTTGCATGAAATGCTAAAAGATATTGATTTAGAAAGCTATAATTCAATACATTTTAATAATAGAAAA
AGAGTAATTAGGGCATTAGAAACTTATAAGTTAACTGGAAAACCATTTTCATCTTTTAAAGCTAAGAATAGTATTTATGA
AACTCCATATAATATATATTATTATGTACTTAATATGGATAGAGCAAAGCTTTATGACAGAATAAACAAAAGAGTTGATA
TAATGTTTGAAAAAGGTCTTTTAGAAGAGGTTAAGAATCTTAAAGCCATGGGATTAACTGATGATATGCAATCAATGAAA
GGTATAGGATACAAAGAAGTTTTATATTATCTTGATGGTAAAATCTCTTTAGAACAATGCATAGAGATGATTAAGCAAGG
GAGCAGAAACTATGCTAAACGTCAGCTTACTTGGTTTAGAAAGGATCCTAGAGCTATATTTATAGATAAAGATACTTTCG
CCTCTGAAGAAGATATTTCTTCCAAAATTATTAATGACATAATAAATTCTTAG

Upstream 100 bases:

>100_bases
CTTTCCACTGTCCACATGGACGTCCAACTATAATTAAATTTACCAGTTATGAATTAGATAAGAAGTTTAAAAGAATAACT
TAAGGAGAGATACAGTTTAA

Downstream 100 bases:

>100_bases
GTTTATAATTAAATTATATTAAAAATTTATGGAGGGCAATAGAATGAATAAGTCAATCAATAACCTACAAGATATATTCT
TAAACAATGCAAGAAAGGAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMDIGSAKISKEEMGGIPHYLIDFVDPSKEFSVAEFKDL
TTEKIKDIQSRGKLPILVGGTGLYINSIICNMNFAESDKDEEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRK
RVIRALETYKLTGKPFSSFKAKNSIYETPYNIYYYVLNMDRAKLYDRINKRVDIMFEKGLLEEVKNLKAMGLTDDMQSMK
GIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQLTWFRKDPRAIFIDKDTFASEEDISSKIINDIINS

Sequences:

>Translated_310_residues
MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMDIGSAKISKEEMGGIPHYLIDFVDPSKEFSVAEFKDL
TTEKIKDIQSRGKLPILVGGTGLYINSIICNMNFAESDKDEEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRK
RVIRALETYKLTGKPFSSFKAKNSIYETPYNIYYYVLNMDRAKLYDRINKRVDIMFEKGLLEEVKNLKAMGLTDDMQSMK
GIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQLTWFRKDPRAIFIDKDTFASEEDISSKIINDIINS
>Mature_310_residues
MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMDIGSAKISKEEMGGIPHYLIDFVDPSKEFSVAEFKDL
TTEKIKDIQSRGKLPILVGGTGLYINSIICNMNFAESDKDEEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRK
RVIRALETYKLTGKPFSSFKAKNSIYETPYNIYYYVLNMDRAKLYDRINKRVDIMFEKGLLEEVKNLKAMGLTDDMQSMK
GIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQLTWFRKDPRAIFIDKDTFASEEDISSKIINDIINS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=33.2268370607029, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1790613, Length=280, Percent_Identity=38.2142857142857, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=32.6666666666667, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=35.3741496598639, Blast_Score=165, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24649830, Length=327, Percent_Identity=30.8868501529052, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35716; Mature: 35716

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMDIGSAKISKEEMGGIPH
CCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCEEEEHHHCCCCCCCCHHHHCCCCH
YLIDFVDPSKEFSVAEFKDLTTEKIKDIQSRGKLPILVGGTGLYINSIICNMNFAESDKD
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHEECCCCCCCCCC
EEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRKRVIRALETYKLTGKPFSSFK
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHEECCCCHHHHH
AKNSIYETPYNIYYYVLNMDRAKLYDRINKRVDIMFEKGLLEEVKNLKAMGLTDDMQSMK
HCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQLTWFRKDPRAIFIDKDTFASEEDIS
CCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEECCCCCCHHHHH
SKIINDIINS
HHHHHHHHCC
>Mature Secondary Structure
MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMDIGSAKISKEEMGGIPH
CCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCEEEEHHHCCCCCCCCHHHHCCCCH
YLIDFVDPSKEFSVAEFKDLTTEKIKDIQSRGKLPILVGGTGLYINSIICNMNFAESDKD
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHEECCCCCCCCCC
EEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRKRVIRALETYKLTGKPFSSFK
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHEECCCCHHHHH
AKNSIYETPYNIYYYVLNMDRAKLYDRINKRVDIMFEKGLLEEVKNLKAMGLTDDMQSMK
HCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQLTWFRKDPRAIFIDKDTFASEEDIS
CCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEECCCCCCHHHHH
SKIINDIINS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA