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The map label for this gene is cobD

Identifier: 18310021

GI number: 18310021

Start: 1239636

End: 1240583

Strand: Direct

Name: cobD

Synonym: CPE1039

Alternate gene names: 18310021

Gene position: 1239636-1240583 (Clockwise)

Preceding gene: 18310020

Following gene: 18310022

Centisome position: 40.89

GC content: 29.22

Gene sequence:

>948_bases
ATGATAAAAATTATAATAGGATATGTTTTAGACCTAATAATAGGAGATCCTAATAATCCATATCACCCTATTAGATATAT
AGGAAAACTAGCATCTAATATGGAAAAGCTTTGGAGAAAGGTTTTCAAGAAAAATCTTAAGATTGCAGGCTTCTTTGCTT
GGCTTTTTATAGTTTTCATAACCTTTGGAGTAACTTTAGGAATTGTACATATTGCTAATAAGATTAATCCTATTTTAGGA
ACTGTAGTATCTGGAATACTTATTTATTTTTGTATTTCTGCTAAGGGGCTTAAAGTTGAAGGACTTAAAGTAATTAAGAT
TTTAAAAGAAGGAGATATTGTTAAGGCTAGAAAACAATTATCTTATATTGTAGGAAGAGATACTGAAAATCTAGATGAAG
AAGCTATAGTTAGAGCTGTAGTGGAAACTGTAGCAGAAAATATGTCAGATGGTATTATAGCACCTTTATTTTTTGCAGGA
ATTGGGGGAGCACCTTTAGCTTTCTTATATAAAGCGGTAAACACATGTGATTCAATGTTTGGGTATAAAAATGAAAAATA
TAAGGATTTTGGATTTTTCTCAGCTAAGCTTGATGATGTGTTTAATTATATTCCAGCTAGATTAACAGCTTATCTTATAG
TAATCTCTTCTTTTATTTTAAGACTTAATTGCAAAAATATTTTTAAAATATACAAAAGAGATAGATATAATCATTCAAGT
CCTAATAGTGCTCATCCAGAAGCAGCAGTTGCAGGAGCTTTAGGAATAAGACTTGGAGGAGCAAATTACTATTTTGGAAA
GCTTGTAGAAAAACCAACTATAGGAGATGCTAAAAAGAAAATAGAAATTTCAGATGTTTATAAGACTAATAATATATTAG
GAATGGTTTCTTTTTTAGGAATGGTAGTAGCACTTATTATTAGATGTATTTTAGAGGTGATTATATGA

Upstream 100 bases:

>100_bases
AACAGGATATCAATAATTTAAAAGGAGATTTTTCAAGTCTAGAGTTTGAAAAAGATATAACAGATAATTTAAGACTAGAA
ATAATAGAAAGGTTTTAGAA

Downstream 100 bases:

>100_bases
ATTTAGGACATGGTGGTAATGTAGAAGAAATTAAGAGGATTTATGGCTTAAAAGAAAATGAAATTATTGATTTTTCAGCT
AATATAAACCCTCTAGGAAT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIKIIIGYVLDLIIGDPNNPYHPIRYIGKLASNMEKLWRKVFKKNLKIAGFFAWLFIVFITFGVTLGIVHIANKINPILG
TVVSGILIYFCISAKGLKVEGLKVIKILKEGDIVKARKQLSYIVGRDTENLDEEAIVRAVVETVAENMSDGIIAPLFFAG
IGGAPLAFLYKAVNTCDSMFGYKNEKYKDFGFFSAKLDDVFNYIPARLTAYLIVISSFILRLNCKNIFKIYKRDRYNHSS
PNSAHPEAAVAGALGIRLGGANYYFGKLVEKPTIGDAKKKIEISDVYKTNNILGMVSFLGMVVALIIRCILEVII

Sequences:

>Translated_315_residues
MIKIIIGYVLDLIIGDPNNPYHPIRYIGKLASNMEKLWRKVFKKNLKIAGFFAWLFIVFITFGVTLGIVHIANKINPILG
TVVSGILIYFCISAKGLKVEGLKVIKILKEGDIVKARKQLSYIVGRDTENLDEEAIVRAVVETVAENMSDGIIAPLFFAG
IGGAPLAFLYKAVNTCDSMFGYKNEKYKDFGFFSAKLDDVFNYIPARLTAYLIVISSFILRLNCKNIFKIYKRDRYNHSS
PNSAHPEAAVAGALGIRLGGANYYFGKLVEKPTIGDAKKKIEISDVYKTNNILGMVSFLGMVVALIIRCILEVII
>Mature_315_residues
MIKIIIGYVLDLIIGDPNNPYHPIRYIGKLASNMEKLWRKVFKKNLKIAGFFAWLFIVFITFGVTLGIVHIANKINPILG
TVVSGILIYFCISAKGLKVEGLKVIKILKEGDIVKARKQLSYIVGRDTENLDEEAIVRAVVETVAENMSDGIIAPLFFAG
IGGAPLAFLYKAVNTCDSMFGYKNEKYKDFGFFSAKLDDVFNYIPARLTAYLIVISSFILRLNCKNIFKIYKRDRYNHSS
PNSAHPEAAVAGALGIRLGGANYYFGKLVEKPTIGDAKKKIEISDVYKTNNILGMVSFLGMVVALIIRCILEVII

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_CLOPE (Q8XLK2)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_561955.1
- GeneID:   989346
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1039
- NMPDR:   fig|195102.1.peg.1102
- HOGENOM:   HBG647131
- OMA:   RWHPLVG
- ProtClustDB:   PRK01209
- BioCyc:   CPER195102:CPE1039-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 35048; Mature: 35048

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc938afc)-; HASH(0xd9d5d70)-; HASH(0xe260cd4)-; HASH(0xe23f4b4)-; HASH(0xd5fded4)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHH
>Mature Secondary Structure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HHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11792842