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The map label for this gene is truA

Identifier: 18309973

GI number: 18309973

Start: 1194072

End: 1194818

Strand: Direct

Name: truA

Synonym: CPE0991

Alternate gene names: 18309973

Gene position: 1194072-1194818 (Clockwise)

Preceding gene: 18309971

Following gene: 18309975

Centisome position: 39.39

GC content: 28.25

Gene sequence:

>747_bases
ATGAGAAATATAAAAATTACTTTAGATTATGATGGGAGTAAGTACAAGGGATGGCAAAAGCAAAACCAAAAGGGAAGTAA
TGTTTCCACAGTTCAAGATAAATTAGAAAAAGTACTAACTAAAATGACTAGTGAAGAGATTCAACTAATTGGTTGTGGAA
GAACTGATAGTGGTGTACATGCAAAAAATTATGTAGCAAATTTTAAGACAAATTCTCTTATGACTCTAGAACAAATTATT
GAATACATAAATGAATATTTACCTGAAGATATAAGGGTAACGGAAATAAGAGAAGCAAGTGAAAGATTTCATGCTAGATT
TAATGTTAAGTCTAAAACTTATGAATATACAATTGATAACAATAAGTTCAAAGATGTATTTTTAAGAAAATATGCATGGC
ATGTTGAAGAAAAGTTAGACCTAGAAGCTATGGAAGAAGGAGCAAAATATTTATTAGGCACTCATGACTTTAAGAGTTTT
ACAAGTTTAAAATCAAAGAACAAATCAACTTTAAGAACCATAAATAGCATTGAATTTCATGAAAATAATAATATATTAAG
TATAAAGATAAATGGAAATGGCTTCTTATTAAATATGGTTAGAATAATGGTAGGAACTTTAGTAGATGTAGGACTTGGAA
AAATTGAACCCAAATATATAAATGATATACTTGAAGCGAAGGAAAGAGCAAAAGCTTCAGAGAAAGCACCAGCTCATGGA
CTTTGTCTTTTAGAATTAAATTATTAA

Upstream 100 bases:

>100_bases
TGATATTCCAATTTAAACATGTTACATTAAAACTTAGATTAAGTATTAAAATTAAAAATTTAAATTTATATATTTAAATT
TTTTTTGAAAGGAAATTAAA

Downstream 100 bases:

>100_bases
ATAAAATCTGTATAAAAGAGTATAAATACTAATTAATAGTTTAACTACTCTTTTATACAGATTATTTTATGCTTCTAATA
AATCTTTTTCTAATTCATTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTSEEIQLIGCGRTDSGVHAKNYVANFKTNSLMTLEQII
EYINEYLPEDIRVTEIREASERFHARFNVKSKTYEYTIDNNKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSF
TSLKSKNKSTLRTINSIEFHENNNILSIKINGNGFLLNMVRIMVGTLVDVGLGKIEPKYINDILEAKERAKASEKAPAHG
LCLLELNY

Sequences:

>Translated_248_residues
MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTSEEIQLIGCGRTDSGVHAKNYVANFKTNSLMTLEQII
EYINEYLPEDIRVTEIREASERFHARFNVKSKTYEYTIDNNKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSF
TSLKSKNKSTLRTINSIEFHENNNILSIKINGNGFLLNMVRIMVGTLVDVGLGKIEPKYINDILEAKERAKASEKAPAHG
LCLLELNY
>Mature_248_residues
MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTSEEIQLIGCGRTDSGVHAKNYVANFKTNSLMTLEQII
EYINEYLPEDIRVTEIREASERFHARFNVKSKTYEYTIDNNKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSF
TSLKSKNKSTLRTINSIEFHENNNILSIKINGNGFLLNMVRIMVGTLVDVGLGKIEPKYINDILEAKERAKASEKAPAHG
LCLLELNY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=245, Percent_Identity=30.6122448979592, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI70166634, Length=271, Percent_Identity=26.1992619926199, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI70166599, Length=271, Percent_Identity=26.1992619926199, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI70166645, Length=271, Percent_Identity=26.1992619926199, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI199559785, Length=260, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=36.3265306122449, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71985046, Length=278, Percent_Identity=25.1798561151079, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321112, Length=232, Percent_Identity=25.8620689655172, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362146, Length=277, Percent_Identity=28.5198555956679, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648455, Length=277, Percent_Identity=28.5198555956679, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007752, Length=298, Percent_Identity=24.8322147651007, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA1_CLOPE (Q8XLQ0)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_561907.1
- ProteinModelPortal:   Q8XLQ0
- SMR:   Q8XLQ0
- GeneID:   989251
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE0991
- NMPDR:   fig|195102.1.peg.1054
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- PhylomeDB:   Q8XLQ0
- ProtClustDB:   PRK12434
- BioCyc:   CPER195102:CPE0991-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28556; Mature: 28556

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTSEEIQLIGCGRTDSGVH
CCCEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
AKNYVANFKTNSLMTLEQIIEYINEYLPEDIRVTEIREASERFHARFNVKSKTYEYTIDN
HHHHHEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCEEEEEECC
NKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSFTSLKSKNKSTLRTINSIEFH
CCHHHHHHHHHHHHHHHHCCHHHHHCCHHHEECCCCHHHHHHHHHCCHHHHHHHHCEEEE
ENNNILSIKINGNGFLLNMVRIMVGTLVDVGLGKIEPKYINDILEAKERAKASEKAPAHG
CCCCEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
LCLLELNY
EEEEEECC
>Mature Secondary Structure
MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTSEEIQLIGCGRTDSGVH
CCCEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
AKNYVANFKTNSLMTLEQIIEYINEYLPEDIRVTEIREASERFHARFNVKSKTYEYTIDN
HHHHHEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCEEEEEECC
NKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSFTSLKSKNKSTLRTINSIEFH
CCHHHHHHHHHHHHHHHHCCHHHHHCCHHHEECCCCHHHHHHHHHCCHHHHHHHHCEEEE
ENNNILSIKINGNGFLLNMVRIMVGTLVDVGLGKIEPKYINDILEAKERAKASEKAPAHG
CCCCEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
LCLLELNY
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842