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The map label for this gene is birA [H]

Identifier: 18309655

GI number: 18309655

Start: 838662

End: 839648

Strand: Direct

Name: birA [H]

Synonym: CPE0673

Alternate gene names: 18309655

Gene position: 838662-839648 (Clockwise)

Preceding gene: 18309654

Following gene: 18309656

Centisome position: 27.67

GC content: 30.8

Gene sequence:

>987_bases
ATGTCGGTTAAAGAGTCAGTACTTAAGATTCTAGAAGAAAATAGGGAGAGAAGTATATCAGGAGAAGAATTGGCTAAACA
TTTATCAGTTTCTAGGGCAGCTATATGGAAGGCTATAAAATCCTTAAGGGGAGAGGGCTACAATATTTCTGCTGTTACCA
ATAGAGGATATCAATTAACAAAGGATAATGACTTAATATCAGCAGAGGGAATAAAAATATTTTTAAATCCTAAATACCGT
GAAAACTATATAAGGGTGTATAAAACTATAGATTCAACTAATCAAGAAGCTAAGAAGCTTTTAATGGATAATGATATACC
ACATGGAACCATATTAATTGCAGAGGAACAAACAGCAGGTAGGGGGAGATTTCAAAGAAAGTTCTTTTCACCTTCTAACA
AAGGAATATATATGAGTGTTATTTTAAGACCTAACATAGAACTTTCAAAGGCCATACATATAACAACCTCAGCAGCCATA
TCTGTTTGTAGGGCAATAGAAACATTAACTAAAAAAAGACCTAAAATAAAGTGGGTTAATGATATATTTTTAGATGATAA
AAAAATTTGCGGCATATTAACAGAGGCAACAGGAAACTTTGAGAGTGGAAGAGTTGAAAATGTTGTTGTTGGAATTGGAG
TGAATTTTAAAACTGATTCAAAAGATTTCCCTGAAGATATAAAAAATATTGCAGGATCAGTATTTGGAGGAGAAAAGCCA
ACTATTACTAGAAATCAGTTAGTTGCTGAGATTTTAAATGAACTTTTTGAATTATGTGAAAATCTAGAGGATAAAAGCAT
AATGACAGAGTATAGAATTTTATCTTTAGTATTAGGAAAAGAAGTAAGTTTTTTAAAGAATAATAAGCTGAATAAAGGAT
TGGCAATTGATATTACTGATGAGGGAGCTTTGGTTGTTAAGTATGAAAATGGTGAAATAGAATACTTAAATTCAGGTGAA
GTATCAGTAAAAACATTAAATAAGTAA

Upstream 100 bases:

>100_bases
ATAATAGGCATAAAAAATATAATCAAGGAGAGTATTAATTAATAATTAAAGAAAAGTAATTTTAATATGAATAATAGAAA
TAAATTAGGGGGCTAGTTAA

Downstream 100 bases:

>100_bases
TTTTAATAATTAAGTAAGAGTGTTAGAAGTAACTATTTCTAAATATGTTTCTAACACTTTTTAAATACATGCTTTTAGCT
TAAAAATACAGTGTTAACAA

Product: bifunctional biotin operon repressor/biotin--[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSVKESVLKILEENRERSISGEELAKHLSVSRAAIWKAIKSLRGEGYNISAVTNRGYQLTKDNDLISAEGIKIFLNPKYR
ENYIRVYKTIDSTNQEAKKLLMDNDIPHGTILIAEEQTAGRGRFQRKFFSPSNKGIYMSVILRPNIELSKAIHITTSAAI
SVCRAIETLTKKRPKIKWVNDIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKTDSKDFPEDIKNIAGSVFGGEKP
TITRNQLVAEILNELFELCENLEDKSIMTEYRILSLVLGKEVSFLKNNKLNKGLAIDITDEGALVVKYENGEIEYLNSGE
VSVKTLNK

Sequences:

>Translated_328_residues
MSVKESVLKILEENRERSISGEELAKHLSVSRAAIWKAIKSLRGEGYNISAVTNRGYQLTKDNDLISAEGIKIFLNPKYR
ENYIRVYKTIDSTNQEAKKLLMDNDIPHGTILIAEEQTAGRGRFQRKFFSPSNKGIYMSVILRPNIELSKAIHITTSAAI
SVCRAIETLTKKRPKIKWVNDIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKTDSKDFPEDIKNIAGSVFGGEKP
TITRNQLVAEILNELFELCENLEDKSIMTEYRILSLVLGKEVSFLKNNKLNKGLAIDITDEGALVVKYENGEIEYLNSGE
VSVKTLNK
>Mature_327_residues
SVKESVLKILEENRERSISGEELAKHLSVSRAAIWKAIKSLRGEGYNISAVTNRGYQLTKDNDLISAEGIKIFLNPKYRE
NYIRVYKTIDSTNQEAKKLLMDNDIPHGTILIAEEQTAGRGRFQRKFFSPSNKGIYMSVILRPNIELSKAIHITTSAAIS
VCRAIETLTKKRPKIKWVNDIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKTDSKDFPEDIKNIAGSVFGGEKPT
ITRNQLVAEILNELFELCENLEDKSIMTEYRILSLVLGKEVSFLKNNKLNKGLAIDITDEGALVVKYENGEIEYLNSGEV
SVKTLNK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=314, Percent_Identity=30.5732484076433, Blast_Score=131, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36766; Mature: 36635

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKESVLKILEENRERSISGEELAKHLSVSRAAIWKAIKSLRGEGYNISAVTNRGYQLT
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
KDNDLISAEGIKIFLNPKYRENYIRVYKTIDSTNQEAKKLLMDNDIPHGTILIAEEQTAG
CCCCEEEECCEEEEECCCHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCC
RGRFQRKFFSPSNKGIYMSVILRPNIELSKAIHITTSAAISVCRAIETLTKKRPKIKWVN
CCHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEH
DIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKTDSKDFPEDIKNIAGSVFGGEKP
HEEECCCHHHEEEEECCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
TITRNQLVAEILNELFELCENLEDKSIMTEYRILSLVLGKEVSFLKNNKLNKGLAIDITD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEC
EGALVVKYENGEIEYLNSGEVSVKTLNK
CCEEEEEEECCCEEEECCCCEEEEECCC
>Mature Secondary Structure 
SVKESVLKILEENRERSISGEELAKHLSVSRAAIWKAIKSLRGEGYNISAVTNRGYQLT
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
KDNDLISAEGIKIFLNPKYRENYIRVYKTIDSTNQEAKKLLMDNDIPHGTILIAEEQTAG
CCCCEEEECCEEEEECCCHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCC
RGRFQRKFFSPSNKGIYMSVILRPNIELSKAIHITTSAAISVCRAIETLTKKRPKIKWVN
CCHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEH
DIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKTDSKDFPEDIKNIAGSVFGGEKP
HEEECCCHHHEEEEECCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
TITRNQLVAEILNELFELCENLEDKSIMTEYRILSLVLGKEVSFLKNNKLNKGLAIDITD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEC
EGALVVKYENGEIEYLNSGEVSVKTLNK
CCEEEEEEECCCEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]