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The map label for this gene is rfbA [H]

Identifier: 18309598

GI number: 18309598

Start: 772458

End: 773339

Strand: Direct

Name: rfbA [H]

Synonym: CPE0616

Alternate gene names: 18309598

Gene position: 772458-773339 (Clockwise)

Preceding gene: 18309597

Following gene: 18309599

Centisome position: 25.48

GC content: 31.63

Gene sequence:

>882_bases
ATGAAAGGAATTATATTAGCTGGTGGAAGTGGGACAAGATTATATCCAGTAACAAAGGCAATGTCAAAGCAAATGGTACC
AATATATGATAAGCCGATGATATATTACCCAATGTCAGTATTAATGTTAGCTGGAATAAGAGAGGTATTAATAATATCAA
CAGAAAGAGATTTACCTAATTTTAAAGAGTTATTTAAAGATGGAAGTGAATTAGGTTTAAATATAGAATACAAAGTTCAA
GAAGCACCAAATGGACTTGCAGAAGCATTTATAATTGGAGAAGAATTCATAGGAGATGACAATGTAGCTATGATTCTAGG
AGACAATATATTCTATGGACAAAACTTCTCAAGTAATTTAAAGGAAGCCGCTGCTTTAGAAAAGGGTGCTATGGTATTTG
GATATTATGTTCAAGATCCTAAGGCCTTTGGAGTAGTAGAATTTGATGAGAATGGAAAGGTAATTTCTTTAGAAGAAAAG
CCAGAACATCCAAAATCAAAGTATGCAGTTCCAGGACTTTATTTCTATGACAATACAGTAATAGAAAAGGCAAAAGGATT
AAAGCCATCTGCAAGAGGAGAACTAGAAATAACTGATTTAAATAGATTATATATGGAAGAAGGAACTTTAAAAGTTAATT
TATTTGGTAGAGGAATGGCTTGGTTAGATACAGGAACTCATGCTTCAATGTTACAAGCATCTAACTTTGTTGAAGCTGTT
CAAAATACTCAAGGTACATATATAGCTTGTTTAGAAGAGATTGCTTATAGAAAAGGATGGATTTCTTCAGAGAAGGTATT
AAACTTAGCTAAACCATTAATGAAAACTGGTTATGGTAAGTATTTAGTTGATATAGTTGAAGAGTTAGAACAAAAAAAAT
AA

Upstream 100 bases:

>100_bases
GGAAAGAAATATGAAAGTTTACCTAGAAGTTTTGTTATGGCTTCTTCAATGAGTAAAAAGGTATATAGTCAAATTTAGGA
TTAAGGTAGAAGGTGAGAAG

Downstream 100 bases:

>100_bases
TAGAGAGATATTAGACGATTATATAAATTTACATAATATAACAAATTCTTATATTATGTAAATTTTTTTGAACTTTATAT
ACAATTTATTGAAAGTGGTG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLAGIREVLIISTERDLPNFKELFKDGSELGLNIEYKVQ
EAPNGLAEAFIIGEEFIGDDNVAMILGDNIFYGQNFSSNLKEAAALEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEK
PEHPKSKYAVPGLYFYDNTVIEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRGMAWLDTGTHASMLQASNFVEAV
QNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKTGYGKYLVDIVEELEQKK

Sequences:

>Translated_293_residues
MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLAGIREVLIISTERDLPNFKELFKDGSELGLNIEYKVQ
EAPNGLAEAFIIGEEFIGDDNVAMILGDNIFYGQNFSSNLKEAAALEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEK
PEHPKSKYAVPGLYFYDNTVIEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRGMAWLDTGTHASMLQASNFVEAV
QNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKTGYGKYLVDIVEELEQKK
>Mature_293_residues
MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLAGIREVLIISTERDLPNFKELFKDGSELGLNIEYKVQ
EAPNGLAEAFIIGEEFIGDDNVAMILGDNIFYGQNFSSNLKEAAALEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEK
PEHPKSKYAVPGLYFYDNTVIEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRGMAWLDTGTHASMLQASNFVEAV
QNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKTGYGKYLVDIVEELEQKK

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=63.4146341463415, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790224, Length=287, Percent_Identity=61.3240418118467, Blast_Score=376, Evalue=1e-106,
Organism=Escherichia coli, GI1787488, Length=293, Percent_Identity=28.6689419795222, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788355, Length=237, Percent_Identity=23.6286919831224, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=25, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355443, Length=233, Percent_Identity=24.4635193133047, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644084, Length=233, Percent_Identity=24.4635193133047, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32665; Mature: 32665

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLAGIREVLIISTERDLPN
CCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCH
FKELFKDGSELGLNIEYKVQEAPNGLAEAFIIGEEFIGDDNVAMILGDNIFYGQNFSSNL
HHHHHCCCHHHCCEEEEEEECCCCCHHHEEEEHHHHCCCCCEEEEEECCEEECCCCCHHH
KEAAALEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEKPEHPKSKYAVPGLYFYDNTV
HHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHH
IEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRGMAWLDTGTHASMLQASNFVEAV
HHHHCCCCCCCCCCEEEEECCEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHH
QNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKTGYGKYLVDIVEELEQKK
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLAGIREVLIISTERDLPN
CCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCH
FKELFKDGSELGLNIEYKVQEAPNGLAEAFIIGEEFIGDDNVAMILGDNIFYGQNFSSNL
HHHHHCCCHHHCCEEEEEEECCCCCHHHEEEEHHHHCCCCCEEEEEECCEEECCCCCHHH
KEAAALEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEKPEHPKSKYAVPGLYFYDNTV
HHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHH
IEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRGMAWLDTGTHASMLQASNFVEAV
HHHHCCCCCCCCCCEEEEECCEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHH
QNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKTGYGKYLVDIVEELEQKK
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]