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The map label for this gene is metK

Identifier: 161485642

GI number: 161485642

Start: 2498554

End: 2499729

Strand: Reverse

Name: metK

Synonym: CPE2177

Alternate gene names: 161485642

Gene position: 2499729-2498554 (Counterclockwise)

Preceding gene: 18311160

Following gene: 18311157

Centisome position: 82.46

GC content: 34.95

Gene sequence:

>1176_bases
ATGAGAAGATTATTTACATCAGAATCAGTTACTGAAGGGCATCCAGATAAAATATGTGACCAAATTTCAGATGCAGTTTT
AGATGCTATATTTGCAAAAGATCCAAACGCAAGAGTTGCTTGTGAAACAGCTGTTACTACAGGATTAGTAATGGTAATGG
GAGAAATAACAACTAATTGTTATGTTGATATCCCTAAAATAGCTAGAGAAACTATAAAGAATATAGGATATGATAGAGCT
AAATACGGTTTCGACTGTGAAACTTGTTCAGTTATGACTACAATAGACGAGCAATCATCAGATATCGCTATGGGTGTTGA
TGAAGCTTTAGAATCAAGAGCTGGAGAAAAAATTGACATAGATGCAGTTGGAGCTGGGGACCAAGGAATGATGTTTGGTT
TTGCTACTAATGAAACTGAAGAATTTATGCCAGCACCAATAGCTATGGCTCACAGATTATCAAGAAGATTAACAGAAGTA
AGAAAGAACGGAACTTTACCTTACTTAAGACCAGACGGAAAAACACAAGTTACTGTTGAATATGAAAATGACAAACCAGT
TAGAATAGATGCAATAGTAATTTCAACACAACATGGTCCAGAAGTATCTCAAGAACAAATACAAGCTGATTTAATGGAAC
ATGTTATAAAAGCTGTTATACCAGCAGAGTTATTAGATGAAAATACTAAGTATTATATAAATCCAACAGGAAGATTCGTT
ATCGGTGGACCTCAAGGAGATGCTGGATTAACAGGAAGAAAGATCATAGTTGATACTTACGGTGGATATGGAAGACACGG
CGGTGGTGCTTTCTCAGGAAAAGACCCAACTAAAGTTGATAGATCAGCTGCATACGCTGCTAGATGGGTAGCTAAAAACT
TAGTTGCTGCAGGAATCGCAGATAAATTAGAAGTTCAAGTTGCTTATGCTATAGGAGTTGCTAAGCCAGTATCAATTATA
GTAGATACTTTCGGAACTGGAAAAATCTCAGACGAAGAAATAGTTAATATAATAAACAAAGTCTTTGATTTAAGACCAGG
AGCTATAATAAGAGATTTAGATTTAAGAAGACCTATATATAGACAAACAGCTGCTTATGGACACTTTGGAAGAACAGATT
TAGATCTTCCATGGGAAAACTTAAACAAAGTTGAAGAAATAAAGAAATATCTTTAA

Upstream 100 bases:

>100_bases
AGATAAATTAAGTTATTTGTTTAAATAAACAGGGTTGGAATAAATAAAAATGAAAGGGGTGAATTAGCTATCTTATTATG
ATAGTAAGATAGCATTAGCC

Downstream 100 bases:

>100_bases
TATATAGAAAAGCTTAGGATTTTATATCCTAAGCTTTTTATTATTTTTCTATAAATTTATTATTTTAAGATGAACTATTA
ATTTTAAGATTAAGATAAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETAVTTGLVMVMGEITTNCYVDIPKIARETIKNIGYDRA
KYGFDCETCSVMTTIDEQSSDIAMGVDEALESRAGEKIDIDAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEV
RKNGTLPYLRPDGKTQVTVEYENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKAVIPAELLDENTKYYINPTGRFV
IGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGIADKLEVQVAYAIGVAKPVSII
VDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRTDLDLPWENLNKVEEIKKYL

Sequences:

>Translated_391_residues
MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETAVTTGLVMVMGEITTNCYVDIPKIARETIKNIGYDRA
KYGFDCETCSVMTTIDEQSSDIAMGVDEALESRAGEKIDIDAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEV
RKNGTLPYLRPDGKTQVTVEYENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKAVIPAELLDENTKYYINPTGRFV
IGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGIADKLEVQVAYAIGVAKPVSII
VDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRTDLDLPWENLNKVEEIKKYL
>Mature_391_residues
MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETAVTTGLVMVMGEITTNCYVDIPKIARETIKNIGYDRA
KYGFDCETCSVMTTIDEQSSDIAMGVDEALESRAGEKIDIDAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEV
RKNGTLPYLRPDGKTQVTVEYENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKAVIPAELLDENTKYYINPTGRFV
IGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGIADKLEVQVAYAIGVAKPVSII
VDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRTDLDLPWENLNKVEEIKKYL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=386, Percent_Identity=60.1036269430052, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=59.7368421052632, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=61.4987080103359, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=58.4856396866841, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17538494, Length=383, Percent_Identity=57.9634464751958, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=57.2164948453608, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17551082, Length=383, Percent_Identity=56.3968668407311, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32565905, Length=383, Percent_Identity=53.0026109660574, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=56.25, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=54.8387096774194, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=56.3106796116505, Blast_Score=341, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=60.2409638554217, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=81.5789473684211, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=60.1049868766404, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=57.9896907216495, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI45552159, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580529, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580537, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580533, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580535, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580531, Length=386, Percent_Identity=56.2176165803109, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=55.1813471502591, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=55.1813471502591, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=54.0106951871658, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=52.8, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CLOP1 (Q0TND4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696856.1
- ProteinModelPortal:   Q0TND4
- SMR:   Q0TND4
- STRING:   Q0TND4
- GeneID:   4203392
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2435
- TIGR:   CPF_2435
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   CPER195103:CPF_2435-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42883; Mature: 42883

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETAVTTGLVMVMGEITTNC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCE
YVDIPKIARETIKNIGYDRAKYGFDCETCSVMTTIDEQSSDIAMGVDEALESRAGEKIDI
EEECHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCEECHHHHHHHHCCCEEEE
DAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEVRKNGTLPYLRPDGKTQVTVE
EEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKAVIPAELLDENTKYYINPTGRFV
ECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCEE
IGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGIA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEVQVAYAIGVAKPVSIIVDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRRPIY
HHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHCCHHH
RQTAAYGHFGRTDLDLPWENLNKVEEIKKYL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETAVTTGLVMVMGEITTNC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCE
YVDIPKIARETIKNIGYDRAKYGFDCETCSVMTTIDEQSSDIAMGVDEALESRAGEKIDI
EEECHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCEECHHHHHHHHCCCEEEE
DAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEVRKNGTLPYLRPDGKTQVTVE
EEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKAVIPAELLDENTKYYINPTGRFV
ECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCEE
IGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGIA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEVQVAYAIGVAKPVSIIVDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRRPIY
HHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHCCHHH
RQTAAYGHFGRTDLDLPWENLNKVEEIKKYL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA