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The map label for this gene is dnaN [H]

Identifier: 17548158

GI number: 17548158

Start: 3713315

End: 3714430

Strand: Reverse

Name: dnaN [H]

Synonym: RSc3441

Alternate gene names: 17548158

Gene position: 3714430-3713315 (Counterclockwise)

Preceding gene: 17548159

Following gene: 17548157

Centisome position: 99.95

GC content: 63.98

Gene sequence:

>1116_bases
ATGCAATTGGTCAAAACCTCGCGAGACAACCTGCTGCGTCCGCTGCAAATCGTGAGCGGCATCGTGGAGCGCCGCCACAC
GTTGCCCATCCTGGCCAACCTGCTGATCCGCAAGAACGGTGAACGGGTCTCCTTCCTGTCCACCGACATCGAAATCCAGA
TCACCACGCACGCCGATTGCGGCGCCGGCAACGGCGACATCGCCACCACGGTGGCCGCGCGCAAGCTGGTCGACATCCTG
CGCGCGATGCCCGATGGCGAAGTCGCGCTCACGCTCAACGACAAGCGCATGAGCGTGCAGTCCGGCAAGAGCCGCTTCGC
GCTGCAGACGCTGGCCGCCGAAGAATTCCCCACCGTGGCCGAGGCCAACGACTTCGGCGCGCGCATCACGCTGCCGCAGA
AGACGCTCAAGCACCTGCTGGCGATGGTGCACTTCGCGATGGCCCAGCAGGACATCCGCTACTACCTGAACGGCATGCTG
CTGGTGGTGGACGGCAAGCAGGTGATGGCCGTGGCCACCGACGGCCACCGCCTGGCGTACTGCGGCGTGGAGACGGGCGA
GCAGCCCGCCGGCGCGGGCGGGCGCCACGAGGTCATCATCCCGCGCAAGACCATTCTCGAGCTCCAGCGCCTGCTGGAAG
ACGTGGACGATCCCGTCTCGGTGCAGCTGGCCTCGAACCAGGTCAAGTTCACCTTCGGCAACATCGAGCTGATCTCCAAG
CTGGTCGAGGGCAAGTTCCCGGATTTCCAGCGCGTGATCCCCAAGGGTTACCGCAACAGCTTCACGATCGACCGCGCGTT
CCTGCAGCAGGCGCTGCAGCGCACGGCCATCCTGACCACCGACAAGTTCAAGGGCGTGCGCTGCATGCTCGACACCAACG
TCCTGAAGATCAGCTCCACCAACGCTGACCAGGAAGAAGCGCAGGAAGAACTCGAAATCGACTACCAGGGCGACGCGCTG
GATATCGGCTTCAACGTCACCTACCTGCTGGACGTGCTGGCCAACCTCAAGGCCGAAAAGGTGCAGGTGAGCCTGGGCGA
CTCCAACTCGAGCGCGCTCATCACCCTGCCGGACGACGACACCTTCAAGTACGTCGTCATGCCGATGCGGATTTGA

Upstream 100 bases:

>100_bases
TCCTTGATTTACAACACGATTTGGAGTTGATCGCAAAGGCTCCCGCCAGCCCAGCCGGGCGAGGGGCGCGCGGTGTCACC
TTAGAAACAAGGATCGCTAC

Downstream 100 bases:

>100_bases
CGAGGCCCGCCCAGCGAAGCCGCGACAGAAACATCGAAAACAAGACGAGGGGCTGGATCGGAACGATCCGCCCCTTCGCC
GTTTTTAAGCAACCCCGCCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADCGAGNGDIATTVAARKLVDIL
RAMPDGEVALTLNDKRMSVQSGKSRFALQTLAAEEFPTVAEANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL
DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI

Sequences:

>Translated_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADCGAGNGDIATTVAARKLVDIL
RAMPDGEVALTLNDKRMSVQSGKSRFALQTLAAEEFPTVAEANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL
DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI
>Mature_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADCGAGNGDIATTVAARKLVDIL
RAMPDGEVALTLNDKRMSVQSGKSRFALQTLAAEEFPTVAEANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL
DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=368, Percent_Identity=45.1086956521739, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41058; Mature: 41058

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
GAGNGDIATTVAARKLVDILRAMPDGEVALTLNDKRMSVQSGKSRFALQTLAAEEFPTVA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHCCCCHHHHHHHHHHHCCCCC
EANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKQVMAVATDGHRLAY
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
EECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCHHHHHH
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISST
HHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCCEEEEEECCEEEEECC
NADQEEAQEELEIDYQGDALDIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDD
CCCHHHHHHHCEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCC
TFKYVVMPMRI
CEEEEEEEECC
>Mature Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKNGERVSFLSTDIEIQITTHADC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
GAGNGDIATTVAARKLVDILRAMPDGEVALTLNDKRMSVQSGKSRFALQTLAAEEFPTVA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHCCCCHHHHHHHHHHHCCCCC
EANDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKQVMAVATDGHRLAY
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVETGEQPAGAGGRHEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK
EECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCHHHHHH
LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISST
HHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCCEEEEEECCEEEEECC
NADQEEAQEELEIDYQGDALDIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDD
CCCHHHHHHHCEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCC
TFKYVVMPMRI
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]