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The map label for this gene is gyrB [H]

Identifier: 17548157

GI number: 17548157

Start: 3710650

End: 3713178

Strand: Reverse

Name: gyrB [H]

Synonym: RSc3440

Alternate gene names: 17548157

Gene position: 3713178-3710650 (Counterclockwise)

Preceding gene: 17548158

Following gene: 17548156

Centisome position: 99.91

GC content: 64.53

Gene sequence:

>2529_bases
ATGACCGAACAGCAGAAACCGCAATCCACACCCGCCGAAAGCAGCAGCTACGGCGCCGCCTCGATCCAGATCCTGGAAGG
CCTGGAGGCGGTGCGCAAGCGGCCGGGCATGTACATCGGCGATACGTCGGACGGCACCGGCCTGCACCACCTCGTGTTCG
AGGTGCTGGACAACTCCATCGACGAGGCGCTGGCGGGGCATTGCACCGAGATCCACGTCACCATCCACACCGACAACTCG
ATCTCCGTGATCGACAACGGCCGCGGCATCCCGACCGGCATCAAGTTCGATGACAAGCACGAGCCGAAGCGCAGCGCGGC
CGAGATCGTCATGACCGAGCTGCACGCCGGCGGCAAGTTCGACCAGAACAGCTACAAGGTGTCGGGCGGCCTGCACGGCG
TGGGTGTGTCGTGCGTGAACGGCCTGTCGAAGTGGCTCAAGCTGACGGTGCGCCGCGACGGCCGCGTGCACCACATGGAA
TTCGCGCGCGGCATCCCGCAGAACCGCCTGCTGGAGCCGGCCGAGGCGCCGGACGGCAAGACGGTCGAAGTGTCGCCGCT
GCGCGTGTCGGGCACCACCGACAAGCGCGGCACCGAAGTGCACTTCCTGGCCGACGAAGAGATCTTCACCAACGTCGAGT
ACCACTACGAGATCCTCTCCAAGCGCATCCGCGAGCTCTCGTTCCTGAACAACGGTGTGCACATCAAGCTGACCGACCAG
CGCACCGGCAAGGAAGAAGACTTCGCCTTCTCGGGCGGCGTGAAGGGCTTCGTCGAGTACATCAACAAGAACAAGACCGT
GCTGCACCCGACCGTCTTCAACGCCACGGGCGAGAAGGACGGCGTGGGCGTGGAAGTGTCGATGCAGTGGAACGACGGCT
TCAACGAGCAGGTGCTCTGCTTCACCAACAACATCCCGCAGCGCGACGGCGGCACCCACCTGACCGGCCTGCGCGCCGCG
ATGACGCGCGTCATCAACAAGTACATCGCCGACAACGAGATCGCCAAGAAGGCCAAGGTCGAAACCTCCGGCGATGACAT
GCGCGAAGGCCTGACCTGCGTGCTGTCGGTGAAGGTGCCCGAGCCCAAGTTCAGCTCGCAGACCAAGGACAAGCTCGTTT
CGTCCGAAGTGCGCCTGCCGGTGGAAGAAGTCGTGGCCAAGGCGCTGGCGGACTTCCTGCTGGAAACGCCCAACGACGCC
AAGATCATCTGCGGCAAGATCGTTGAAGCCGCCCGCGCCCGCGAAGCCGCCCGCAAGGCCCGCGAGATGACGCGCCGCAA
GGGCGTGCTCGACGGCATGGGCCTGCCCGGCAAGCTGGCCGACTGCCAGGAGAAAGACCCGGCGCTGTCCGAACTGTTCA
TCGTCGAGGGTGACTCCGCAGGCGGCTCGGCCAAGCAGGGCCGCGACCGCAAGTTCCAGGCGATCCTGCCGCTCAAGGGC
AAGATCCTGAACGTGGAACGCGCGCGCTTCGACAAGATGCTCTCCAGCCAGGAAGTGCTCACGCTCATCACCGCCATGGG
CACCGGCATCGGCAAGGACGACTACAACCTCGACAAGCTGCGCTACCACCGCATCATCATCATGACCGACGCGGACGTGG
ACGGCTCGCACATCCGCACGCTGCTGCTGACGTTCTTCTACCGCCAGATGCCCGAGATCATCGAGCGCGGCCACGTGTAC
ATCGCCCAGCCGCCGCTGTACAAGATCAAGCACGGCAAGGAAGAGCGCTACATCAAGGACGACGTCGAGATGGCCGCGTA
CCTGATGCGCCAGGCCCTCGACACCGCCATCCTGGTGCGCGCCGACGGCACCGAGATCGCCAGCGACGCGCTGGCCGAGC
TGGCGCGGCAGTACCAGTTCTCGCGCGCCGTGATCGAGCGACTGTCGCGCGTGATCGATGCGGATGCGCTGCGCGCCATT
GCCGAAGGCGTGGCGCTGGACCTGTCCAGCGAAGCGGGCGCCGAAGCCAGCGCCAAGGCGCTCAAGGCGCGGCTGCTGGA
AATGCAGGGCAACGCCAGCAGCGCCAACGGTGGCGCGACGGCGGATGCGTTCATGCAGTACGACGAGAAGCACGAGAAGT
ACCGCGTGATGGTCGTGCGCCGCCAGCACGGCAACCAACGCCTATCGCACATCGACGCCGACTTCGTGGCCGGCGCCGAC
TACGCCACGCTGTCGCAGACTGCGCAGACCTTCCAGGGGTTGATCGGGGAGGGGGCCAAGGTGCGGCGTGGGACTGGGGA
TAAGCAGCGCGAGCAGGGTGTGACGGACTTCCATGCGGCGATTACTTGGCTGCTGGGTGAGGCTGAGCGTGGCATCAGCC
GGCAACGTTATAAGGGGCTGGGGGAGATGAACCCCTCGCAGCTGTGGGAGACCACTATGGATGTTACGCAGCGGCGACTG
CTCAAGGTGCAGATTGAAGACGCTATTGCGGCGGATCAGATCTTTACTACGCTGATGGGGGATGACGTGGAGCCTCGGCG
GAACTTTATTGAGAGTAATGCGTTGGTGGCGCGGAATATTGATGTTTGA

Upstream 100 bases:

>100_bases
AAAACAAGACGAGGGGCTGGATCGGAACGATCCGCCCCTTCGCCGTTTTTAAGCAACCCCGCCATGTGCGCCCGACGCCC
ATGGCTGCGAGCAGAACGAC

Downstream 100 bases:

>100_bases
GGGGTTGGTTCACTCAGACGATATGGAGTGAGCGGGCAGCGCGTTAGTTGTATCTAGTCTGGCATTGAGGGGGCGTTCGT
ATGGACACCATATCGAAGGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MTEQQKPQSTPAESSSYGAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIHVTIHTDNS
ISVIDNGRGIPTGIKFDDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNGLSKWLKLTVRRDGRVHHME
FARGIPQNRLLEPAEAPDGKTVEVSPLRVSGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIKLTDQ
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTVFNATGEKDGVGVEVSMQWNDGFNEQVLCFTNNIPQRDGGTHLTGLRAA
MTRVINKYIADNEIAKKAKVETSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALADFLLETPNDA
KIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVY
IAQPPLYKIKHGKEERYIKDDVEMAAYLMRQALDTAILVRADGTEIASDALAELARQYQFSRAVIERLSRVIDADALRAI
AEGVALDLSSEAGAEASAKALKARLLEMQGNASSANGGATADAFMQYDEKHEKYRVMVVRRQHGNQRLSHIDADFVAGAD
YATLSQTAQTFQGLIGEGAKVRRGTGDKQREQGVTDFHAAITWLLGEAERGISRQRYKGLGEMNPSQLWETTMDVTQRRL
LKVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV

Sequences:

>Translated_842_residues
MTEQQKPQSTPAESSSYGAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIHVTIHTDNS
ISVIDNGRGIPTGIKFDDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNGLSKWLKLTVRRDGRVHHME
FARGIPQNRLLEPAEAPDGKTVEVSPLRVSGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIKLTDQ
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTVFNATGEKDGVGVEVSMQWNDGFNEQVLCFTNNIPQRDGGTHLTGLRAA
MTRVINKYIADNEIAKKAKVETSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALADFLLETPNDA
KIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVY
IAQPPLYKIKHGKEERYIKDDVEMAAYLMRQALDTAILVRADGTEIASDALAELARQYQFSRAVIERLSRVIDADALRAI
AEGVALDLSSEAGAEASAKALKARLLEMQGNASSANGGATADAFMQYDEKHEKYRVMVVRRQHGNQRLSHIDADFVAGAD
YATLSQTAQTFQGLIGEGAKVRRGTGDKQREQGVTDFHAAITWLLGEAERGISRQRYKGLGEMNPSQLWETTMDVTQRRL
LKVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV
>Mature_841_residues
TEQQKPQSTPAESSSYGAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIHVTIHTDNSI
SVIDNGRGIPTGIKFDDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNGLSKWLKLTVRRDGRVHHMEF
ARGIPQNRLLEPAEAPDGKTVEVSPLRVSGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIKLTDQR
TGKEEDFAFSGGVKGFVEYINKNKTVLHPTVFNATGEKDGVGVEVSMQWNDGFNEQVLCFTNNIPQRDGGTHLTGLRAAM
TRVINKYIADNEIAKKAKVETSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALADFLLETPNDAK
IICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKGK
ILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVYI
AQPPLYKIKHGKEERYIKDDVEMAAYLMRQALDTAILVRADGTEIASDALAELARQYQFSRAVIERLSRVIDADALRAIA
EGVALDLSSEAGAEASAKALKARLLEMQGNASSANGGATADAFMQYDEKHEKYRVMVVRRQHGNQRLSHIDADFVAGADY
ATLSQTAQTFQGLIGEGAKVRRGTGDKQREQGVTDFHAAITWLLGEAERGISRQRYKGLGEMNPSQLWETTMDVTQRRLL
KVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=566, Percent_Identity=24.9116607773852, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI19913406, Length=552, Percent_Identity=24.6376811594203, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI48994957, Length=834, Percent_Identity=60.0719424460432, Blast_Score=965, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=569, Percent_Identity=39.5430579964851, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=610, Percent_Identity=24.5901639344262, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=521, Percent_Identity=24.3761996161228, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=34.1935483870968, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=23.4113712374582, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17136538, Length=554, Percent_Identity=23.826714801444, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93021; Mature: 92889

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQQKPQSTPAESSSYGAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
DEALAGHCTEIHVTIHTDNSISVIDNGRGIPTGIKFDDKHEPKRSAAEIVMTELHAGGKF
HHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
DQNSYKVSGGLHGVGVSCVNGLSKWLKLTVRRDGRVHHMEFARGIPQNRLLEPAEAPDGK
CCCCEEECCCCCCCHHHHHHHHHHHHEEHHHCCCCEEHHHHHCCCCHHHCCCCCCCCCCC
TVEVSPLRVSGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIKLTDQ
EEEEEEEEEECCCCCCCCEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTVFNATGEKDGVGVEVSMQWNDGFNEQVLC
CCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEE
FTNNIPQRDGGTHLTGLRAAMTRVINKYIADNEIAKKAKVETSGDDMREGLTCVLSVKVP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCEEEEEEECC
EPKFSSQTKDKLVSSEVRLPVEEVVAKALADFLLETPNDAKIICGKIVEAARAREAARKA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
REMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCC
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRT
EEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
LLLTFFYRQMPEIIERGHVYIAQPPLYKIKHGKEERYIKDDVEMAAYLMRQALDTAILVR
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
ADGTEIASDALAELARQYQFSRAVIERLSRVIDADALRAIAEGVALDLSSEAGAEASAKA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCCHHHHHH
LKARLLEMQGNASSANGGATADAFMQYDEKHEKYRVMVVRRQHGNQRLSHIDADFVAGAD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCHHHHCCCC
YATLSQTAQTFQGLIGEGAKVRRGTGDKQREQGVTDFHAAITWLLGEAERGISRQRYKGL
HHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCC
GEMNPSQLWETTMDVTQRRLLKVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNI
CCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECC
DV
CC
>Mature Secondary Structure 
TEQQKPQSTPAESSSYGAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
DEALAGHCTEIHVTIHTDNSISVIDNGRGIPTGIKFDDKHEPKRSAAEIVMTELHAGGKF
HHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
DQNSYKVSGGLHGVGVSCVNGLSKWLKLTVRRDGRVHHMEFARGIPQNRLLEPAEAPDGK
CCCCEEECCCCCCCHHHHHHHHHHHHEEHHHCCCCEEHHHHHCCCCHHHCCCCCCCCCCC
TVEVSPLRVSGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIKLTDQ
EEEEEEEEEECCCCCCCCEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTVFNATGEKDGVGVEVSMQWNDGFNEQVLC
CCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEE
FTNNIPQRDGGTHLTGLRAAMTRVINKYIADNEIAKKAKVETSGDDMREGLTCVLSVKVP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCEEEEEEECC
EPKFSSQTKDKLVSSEVRLPVEEVVAKALADFLLETPNDAKIICGKIVEAARAREAARKA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
REMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCC
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRT
EEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
LLLTFFYRQMPEIIERGHVYIAQPPLYKIKHGKEERYIKDDVEMAAYLMRQALDTAILVR
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
ADGTEIASDALAELARQYQFSRAVIERLSRVIDADALRAIAEGVALDLSSEAGAEASAKA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCCHHHHHH
LKARLLEMQGNASSANGGATADAFMQYDEKHEKYRVMVVRRQHGNQRLSHIDADFVAGAD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCHHHHCCCC
YATLSQTAQTFQGLIGEGAKVRRGTGDKQREQGVTDFHAAITWLLGEAERGISRQRYKGL
HHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCC
GEMNPSQLWETTMDVTQRRLLKVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNI
CCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECC
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]