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The map label for this gene is priA [H]

Identifier: 17548019

GI number: 17548019

Start: 3562909

End: 3565215

Strand: Reverse

Name: priA [H]

Synonym: RSc3302

Alternate gene names: 17548019

Gene position: 3565215-3562909 (Counterclockwise)

Preceding gene: 17548020

Following gene: 17548018

Centisome position: 95.93

GC content: 73.73

Gene sequence:

>2307_bases
ATGACCGCTACCCGTGTTGATGCCGCTCCGCCCGCCGTTGCCCAGGCAGCGCGCGCGGGCGCCATTGCGCGGGTGGTGAT
CGATACGCCGCTGGATGCCGTATTCGACTATCGCTGCGCGCAGCCGGTCGTGCCGGGCCAGCTGGTGGTGGTGCCGTTCG
GCAGCCGGCGCGTGGTGGCGCTGGTGGTGGAGAGCGCCGGCACCACCGAGGTGCCGGCAGACCGCCTGCGCGATGTCGAG
CGCGTGCTCGACTGGGTGCCGCCGCTGGATGCCGAATGGCGCGCGCTGGCCGAATTCGCCGCGGGCTACTACCACCGGGC
GCTGGGTGAAGTGACGCTGCCGGCGCTGCCGCCGTTGTGGCGTACGCCCGGCAGCTGGGACAGCCTGGCCCGCCATGCCA
CCGAGACCGTCTACGTGATGCGCGAGCCGGCGCGCGCCGCCTTGCTCGACAGCATCCCCCGGCGGGCGGCCGGGGCGATG
CGCCTGGCCGAAGCGCTGACGGGCGACGGCGAACTGTCCGCCAGCGCCGCTGTCGCGCTGCACGGGCAGGCGACGGCCAA
GCTGCGCGATTGGGCCGCGGTGGGCTGGGTCGGGGCGGAACTGCGTCCCAGGCCGTTGCTGCCGGTGCCGCGCGATCTGC
CGCCGGCGTCCGTCGCGCCGGCGCTCAATGCCGAGCAGGCTGCTGCCGTCGAGGCCATCGCGCAGGCCGGGGCGGGCGGC
GGTTTCGCGCCGTTCCTGCTGTACGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCACGCCATCGCCGATGCCCT
CGCGCGCGATCCGGCCGCGCAGGTGCTGATGCTGGTGCCCGAGATCAACCTGACGCCGCAGCTGGAGGCGCGCATTGCGG
CGCGCTTCCCCGGCGTGCCGCTGGCCGCGCTGCACAGCGGCCTGGCCGAGCAGCCGCGCGCGCTGAACTGGCTGGCGGCG
CATCGCGGCGAGGCGCGCATCGTGCTCGGCACGCGGCTGGCGATGCTGGCCTCGCTGCCGCACCTCGCGCTGATCCTCGT
CGACGAGGAGCACGACACGTCCTATAAGCAGCAAGAGGGCCTGCGCTATTCCGCGCGCGATCTGGCGCTGTGGCGGGCCA
AGCAGCGCGGCATCCCGGTCGTGCTCGGGTCGGCCACGCCGTCGCTGGAGACCTGGTGGCGCGCCGAGCAGGGCACCACC
GCGCGCCTGGTGCTGTCGCAGCGCGCGCAGGCCGATGCGGTGCTGCCGCGCGTCTCGCTGATCGACCTGACGCTGGAGCG
CCGCGCGGGCCGGGAGATCCGCGACGGGCTGTCCAGGCCGCTGCTCGATGCCGTTGCCGGGCGCCTGGCGCGCGGCGAGC
AGAGCCTGCTGTTCCTCAACCGCCGCGGCTACGCGCCGGTGCTGGCGTGCGACGCCTGCGGCTGGTTGTCGGGCTGCCCG
CGCTGCTCGGCGTATCTGGTGCTGCACAAGCCGGAGCACCGGCTGCGCTGCCACCACTGCGGCTACGAATCGCGCATCCC
GCACCATTGCCCGGACTGCGGCAACGTCGACATCGCGCCGCTGGGGCGCGGCACGCAGCGCATCGAGGAGACCCTCGGCG
CGCTGTTTCCGCAGGCGCGCGTGGCGCGCATCGACGCCGACTCCACCCGCCGCAAGGGCAGCGCCGAGGCGCTGTTCGAC
ACCGTGCACGAAGGGGCCGTCGATATCCTGATCGGCACGCAGATGGTCGCCAAGGGGCACGACTTTCAGCGCGTGACGCT
GGTGGGCGTGGTGGCGCCCGATGCGTCCCTGTTCAGCCACGACTTCCGTGCCGGCGAGCACCTGTTCGCCTCGCTGATGC
AGGTGGCCGGGCGCGCCGGCCGTGCCGGGCTGGCGGGCGAAGTGCTGATCCAGACCCGCTACCCGGATGCCCCCGCCCTG
CTGGCGCTGGTGCGGCACGATTACGCCGGCTTCGCGCGCCAGCTGCTGCGCGAGCGCAAGCAGGCCTGCCTGCCGCCGTT
CGCCTACCAGGCGCTGCTGACCGCCGAGCACCGCGAGTTGGCGCGCGCGCTGGAATTCCTCGGCGCCGCGCGCGCGGCCG
GGCAGGCCGTGGCCGAAGCCCAGAGCCTGCCGGTGCAACTGCACGATCCGGTGCCCATGACCATGGTGCGCCTGGCCAAC
CGCGAACGCGCCCAACTGCTGCTCGAAAGCGCTTCGCGCGCCGCCCTGCAGCGGCTGCTGGCCACCTGGACCGACGGGTT
TGGCGACATCGGCCGGACGGTCAAGGGCGTGCGCTGGCAGCTCGAGATCGACCCGCTGCGGATCTGA

Upstream 100 bases:

>100_bases
GGCGTCACGTTCGCGGTGGGGCCGGCGGATCGCTTTTCCGGGTGCGCTGGGCGCCGCTGCTTCGCAACAAATCCTGTGCA
ATTCACCGCCTGTCCCACGC

Downstream 100 bases:

>100_bases
TCACCACCTCCGGTGCGGGGTGCGTCATGTGCGGTTGCACCAAAAATCCGGTGCTCTGAACCAAGGGGTTCTACTAAGTT
GCACCCCGCGAGCGGCCGGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVESAGTTEVPADRLRDVE
RVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAM
RLAEALTGDGELSASAAVALHGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAA
HRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTT
ARLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQARVARIDADSTRRKGSAEALFD
TVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPAL
LALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLAN
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI

Sequences:

>Translated_768_residues
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVESAGTTEVPADRLRDVE
RVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAM
RLAEALTGDGELSASAAVALHGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAA
HRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTT
ARLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQARVARIDADSTRRKGSAEALFD
TVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPAL
LALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLAN
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI
>Mature_767_residues
TATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVESAGTTEVPADRLRDVER
VLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAMR
LAEALTGDGELSASAAVALHGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGGG
FAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAAH
RGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTTA
RLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCPR
CSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQARVARIDADSTRRKGSAEALFDT
VHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALL
ALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLANR
ERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=765, Percent_Identity=39.4771241830065, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82845; Mature: 82714

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVA
CCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
LVVESAGTTEVPADRLRDVERVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLW
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAMRLAEALTGDGELSASAAVAL
CCCCCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
HGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
CCCHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVP
CCCHHHEEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHCCCCC
LAALHSGLAEQPRALNWLAAHRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEG
HHHHHCCHHCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHCC
LRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTTARLVLSQRAQADAVLPRVSL
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHCCCHHH
IDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
HHHHHHHHCCHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCHHHCCCC
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQAR
CCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VARIDADSTRRKGSAEALFDTVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSH
EEEECCCHHCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHH
DFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALLALVRHDYAGFARQLLRERK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHC
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCC
>Mature Secondary Structure 
TATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVA
CCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
LVVESAGTTEVPADRLRDVERVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLW
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAMRLAEALTGDGELSASAAVAL
CCCCCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
HGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
CCCHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVP
CCCHHHEEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHCCCCC
LAALHSGLAEQPRALNWLAAHRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEG
HHHHHCCHHCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHCC
LRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTTARLVLSQRAQADAVLPRVSL
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHCCCHHH
IDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
HHHHHHHHCCHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCHHHCCCC
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQAR
CCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VARIDADSTRRKGSAEALFDTVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSH
EEEECCCHHCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHH
DFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALLALVRHDYAGFARQLLRERK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHC
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]